| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2350640 | B | Inhibition of recombinant full length human NNMT measured for 30 mins by SAHH-coupled fluorescence assay | Homo sapiens | 1 | ALA2346640 | single protein format | Scientific Literature | |
| 2. | ALA4028398 | B | Inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) using nicotinamide/SAM as substrate/co-factor measured for 20 mins by SAHH-coupled fluorescent assay | Homo sapiens | 2 | ALA4024794 | assay format | Scientific Literature | |
| 3. | ALA4028399 | B | Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) by isothermal titration calorimetric method | Homo sapiens | 2 | ALA4024794 | assay format | Scientific Literature | |
| 4. | ALA4028404 | B | Competitive inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) at 1 to 250 uM measured for 20 mins in presence of varying concentration of SAM by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4024794 | assay format | Scientific Literature | |
| 5. | ALA4028405 | B | Nonompetitive inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) at 1 to 250 uM measured for 20 mins in presence of varying concentration of nicotinamide by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4024794 | assay format | Scientific Literature | |
| 6. | ALA4028406 | B | Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in enthalpy by isothermal titration calorimetric method | Homo sapiens | 2 | ALA4024794 | assay format | Scientific Literature | |
| 7. | ALA4028407 | B | Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in entropy by isothermal titration calorimetric method | Homo sapiens | 2 | ALA4024794 | assay format | Scientific Literature | |
| 8. | ALA4188596 | B | Inhibition of human NNMT at 30 uM preincubated for 30 mins followed by nicotinamide substrate and SAM addition measured after 60 mins by fluorescence HTS assay | Homo sapiens | 1 | ALA4184280 | single protein format | Scientific Literature | |
| 9. | ALA4188597 | B | Inhibition of human NNMT at 30 uM preincubated for 30 mins followed by nicotinamide substrate and SAM addition measured after 60 mins by fluorescence assay | Homo sapiens | 22 | ALA4184280 | single protein format | Scientific Literature | |
| 10. | ALA4188599 | B | Inhibition of NNMT in human U2OS cells assessed as reduction in MNA production after 24 hrs by LC-MS/MS analysis | Homo sapiens | 2 | ALA4184280 | cell-based format | Scientific Literature | |
| 11. | ALA4232620 | B | Inhibition of SAH probe binding to NNMT (unknown origin) | Homo sapiens | 1 | ALA4229400 | single protein format | Scientific Literature | |
| 12. | ALA4232621 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate at 2 uM preincubated for 1 hr followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 1 | ALA4229400 | assay format | Scientific Literature | |
| 13. | ALA4232622 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate at 2 to 20 uM preincubated for 1 hr followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 26 | ALA4229400 | assay format | Scientific Literature | |
| 14. | ALA4232623 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate at 0.5 to 2 uM preincubated for 1 hr followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 38 | ALA4229400 | assay format | Scientific Literature | |
| 15. | ALA4232624 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate preincubated for 1 hr followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 11 | ALA4229400 | assay format | Scientific Literature | |
| 16. | ALA4232625 | B | Inhibition of NNMT in human 786-O cell lysate using d4-nicotinamide as substrate preincubated for 1 hr followed by substrate and SAM addition measured after 15 to 30 mins by LC-MS analysis | Homo sapiens | 3 | ALA4229400 | assay format | Scientific Literature | |
| 17. | ALA4232626 | B | Inhibition of NNMT C165A mutant in human 786-O cell lysate using d4-nicotinamide as substrate preincubated for 1 hr followed by substrate and SAM addition measured after 15 to 30 mins by LC-MS analysis | Homo sapiens | 2 | ALA4229400 | assay format | Scientific Literature | |
| 18. | ALA4232627 | B | Inhibition of SAH-rhodamine binding to NNMT in human 786-O cell soluble proteomic lysate assessed as ratio of observed rate constant to test compound concentration preincubated for 10 to 40 mins followed by SAH-rhodamine addition measured after 5 mins by Gel-based ABPP analysis | Homo sapiens | 2 | ALA4229400 | assay format | Scientific Literature | |
| 19. | ALA4232628 | B | Inhibition of NNMT in human 786-O cell lysate using d4-nicotinamide as substrate pretreated for 1 hr at 3 mins post 160 uM SAM addition followed by substrate addition measured after 15 to 30 mins by LC-MS analysis | Homo sapiens | 1 | ALA4229400 | assay format | Scientific Literature | |
| 20. | ALA4232629 | B | Inhibition of NNMT in human 786-O cell lysate using d4-nicotinamide as substrate pretreated for 1 hr at 3 mins post 80 uM SAM addition followed by substrate addition measured after 15 to 30 mins by LC-MS analysis | Homo sapiens | 1 | ALA4229400 | assay format | Scientific Literature |
The page will load shortly, Thanks for your patience!