# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2354285 | Functional | PubChem BioAssay. FRET-based HTS for detection of RAD52 Inhibitors Measured in Biochemical System Using Plate Reader - 7018-01_Inhibitor_Dose_CherryPick_Activity. (Class of assay: confirmatory) | 635 | ALA1201862 | biochemical format | PubChem BioAssays | ||
2. | ALA3215214 | Functional | PubChem BioAssay. Effect of RAD 52 Inhibitors on Growth of CAPAN-1 Cells Measured in Cell-Based System Using Imaging - 7018-03_Inhibitor_Dose_DryPowder_Activity_Set3. (Class of assay: confirmatory) | 34 | ALA1201862 | cell-based format | PubChem BioAssays | ||
3. | ALA3214880 | Functional | PubChem BioAssay. Effect of RAD 52 Inhibitors on Growth of CAPAN-1 Cells Measured in Cell-Based System Using Imaging - 7018-03_Inhibitor_Dose_DryPowder_Activity_Set2. (Class of assay: confirmatory) | 28 | ALA1201862 | cell-based format | PubChem BioAssays | ||
4. | ALA4417894 | Binding | Inhibition of RAD52 (unknown origin) assessed as effect on ssDNA annealing using ssDNA oligonucleotide by fluorescence-quenching assay | Homo sapiens | 17 | ALA4417629 | single protein format | Patent Bioactivity Data | |
5. | ALA4417895 | Binding | Inhibition of RAD52 (unknown origin) assessed as inhibition of DNA pairing using [32P]-ssDNA by measuring D-loop formation incubated for 15 mins by gel electrophoresis method | Homo sapiens | 17 | ALA4417629 | single protein format | Patent Bioactivity Data | |
6. | ALA4417899 | Binding | Binding affinity to RAD52 (unknown origin) by SPR assay | Homo sapiens | 2 | ALA4417629 | single protein format | Patent Bioactivity Data | |
7. | ALA4417900 | Binding | Inhibition of RAD52 (unknown origin) expressed in BCR-ABL1-positive and BRCA1-deficient mouse 32Dcl3 cells expressing GFP-RAD52 assessed as reduction in cisplatin-induced RAD52 foci formation at 2.5 uM by AlexaFluor-594 staining based inverted fluorescence microscopy relative to control | Homo sapiens | 2 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
8. | ALA4417901 | Binding | Inhibition of RAD52 (unknown origin) expressed in BCR-ABL1-positive and BRCA1-deficient mouse 32Dcl3 cells expressing GFP-RAD52 assessed as cisplatin-induced RAD52 foci formation at 2.5 uM by AlexaFluor-594 staining based inverted fluorescence microscopy (Rvb = 38.7 +/- 10%) | Homo sapiens | 2 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
9. | ALA4417902 | Binding | Inhibition of RAD52 (unknown origin) expressed in BCR-ABL1-positive and BRCA1-deficient mouse 32Dcl3 cells expressing GFP-RAD52 assessed as cisplatin-induced RAD52 foci formation by measuring fraction of cell without foci at 2.5 uM by AlexaFluor-594 staining based inverted fluorescence microscopy (Rvb = 48.4 +/- 6.2%) | Homo sapiens | 2 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
10. | ALA4417905 | ADME | Effect on RAD52 (unknown origin) expressed in BCR-ABL1-positive and BRCA1-deficient mouse 32Dcl3 cells expressing GFP-RAD52 assessed as induction of RAD52 foci formation by AlexaFluor-594 staining based inverted fluorescence microscopy | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
11. | ALA4417906 | Binding | Inhibition of RAD52 in human U2OS cells co-expressing SA-GFP assessed as reduction in single strand DNA annealing incubated for 48 hrs by GFP+ positive cells detection based flow cytometry | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
12. | ALA4417907 | Binding | Inhibition of RAD52 in human U2OS cells co-expressing SA-GFP assessed as reduction in single strand DNA annealing at 30 uM incubated for 48 hrs by GFP+ positive cells detection based flow cytometry relative to control | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
13. | ALA4417911 | Binding | Inhibition of RAD52 in human U2OS cells co-expressing SSA-GFP assessed as inhibition of repair of I-Scel-induced double stranded breaks by measuring green fluorescence at 5 uM by flow cytometry (Rvb = 7.1%) | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
14. | ALA4417912 | Binding | Inhibition of RAD52 in human U2OS cells co-expressing SSA-GFP assessed as inhibition of repair of I-Scel-induced double stranded breaks by measuring green fluorescence at 10 uM by flow cytometry (Rvb = 7.1%) | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
15. | ALA4417913 | Binding | Inhibition of RAD52 in human U2OS cells co-expressing SSA-GFP assessed as inhibition of repair of I-Scel-induced double stranded breaks by measuring green fluorescence at 15 uM by flow cytometry (Rvb = 7.1%) | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
16. | ALA4417914 | Binding | Inhibition of RAD52 in human U2OS cells co-expressing SSA-GFP assessed as inhibition of repair of I-Scel-induced double stranded breaks by measuring green fluorescence at 20 uM by flow cytometry (Rvb = 7.1%) | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
17. | ALA4417915 | Binding | Inhibition of RAD52 in human U2OS cells co-expressing SSA-GFP assessed as inhibition of repair of I-Scel-induced double stranded breaks by measuring green fluorescence at 30 uM by flow cytometry (Rvb = 7.1%) | Homo sapiens | 1 | ALA4417629 | cell-based format | Patent Bioactivity Data | |
18. | ALA4482620 | Binding | Inhibition of RAD52 in hTERT-immortalized wild-type human GM01604 fibroblasts with BRCA2 knockdown assessed as potentiation of HU and UCN01-induced cell death by Live/Dead assay | Homo sapiens | 1 | ALA4482312 | single protein format | Patent Bioactivity Data | |
19. | ALA4482621 | Binding | Inhibition of RAD52 (unknown origin) assessed as reduction in RAD52-ssDNA interaction using Cy3-dT30-Cy5 ssDNA by FRET assay | Homo sapiens | 26 | ALA4482312 | single protein format | Patent Bioactivity Data | |
20. | ALA4482622 | Binding | Inhibition of RAD52 (unknown origin) assessed as reduction in RAD52-ssDNA interaction using Cy3-dT30-Cy5 ssDNA in presence of replication protein A by FRET assay | Homo sapiens | 3 | ALA4482312 | single protein format | Patent Bioactivity Data |