#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3887059	B	Cell Free Inhibition Assay: The inhibitory concentrations (IC50, [uM]) of the beta -lactamase inhibitors against purified TEM-1, SHV-1 and AmpC beta -lactamases are assessed by determining the concentration of inhibitor at which 50% of the nitrocefin hydrolysis is inhibited by the particular enzyme. Assays are performed with beta -lactamases expressed in the pET system (Novagen, San Diego, Calif.) without signal peptides. They contain an N-terminal hexa-Histidine tag that is used for purification on Ni-NTA (Qiagen, Hilden, Germany). The compounds are prepared as 50 mM stocks in DMSO and diluted into buffer P1 (50 mM phosphate, pH 7) to a final concentration of 10% DMSO. All further dilutions are done in P2 (P1 with 10% DMSO). Enzyme and compound dilutions are pre-incubated for 10 min at 37 C. and the reaction is started with the addition of pre-warmed (37 C.) nitrocefin at a final concentration of 50 mM. The change in absorption at 490 nm is followed at 37 C. for 10 min with 30 s intervals.		42	ALA3886147	assay format	BindingDB Database
2.	ALA4027949	B	Inhibition of recombinant N-terminal His-tagged bacterial Escherichia coli TEM-1 (24 to 286 residues) expressed in Escherichia coli BL21 (DE3) cells using FC5 as substrate preincubated up to 360 mins prior to substrate addition by fluorescence-based assay	Escherichia coli	2	ALA4024786	cell-based format	Scientific Literature
3.	ALA4121762	B	Inhibition of recombinant N-terminal His-tagged Escherichia coli TEM1 (24 to 286 residues) expressed in Escherichia coli BL21(DE3) using FC5 as substrate assessed as residual activity at 200 uM	Escherichia coli	2	ALA4118127	assay format	Scientific Literature
4.	ALA4121766	B	Inhibition of recombinant N-terminal His-tagged Escherichia coli TEM1 (24 to 286 residues) expressed in Escherichia coli BL21(DE3) at 200 uM using FC5 as substrate	Escherichia coli	57	ALA4118127	assay format	Scientific Literature
5.	ALA4322458	B	Inhibition of recombinant bacterial class A serine beta-lactamase TEM-1 expressed in Escherichia coli assessed as reduction in breakdown of cephalosporin FC-5 preincubated for 10 mins followed by cephalosporin FC-5 addition by fluorescence method	Bacteria	1	ALA4321803	assay format	Scientific Literature
6.	ALA4351600	B	Inhibition of bacterial beta-lactamase TEM-1	Bacteria	1	ALA4350960	single protein format	Scientific Literature
7.	ALA4367635	B	Inhibition of bacterial beta lactamase TEM-1	Bacteria	1	ALA4364300	single protein format	Scientific Literature
8.	ALA4367666	B	Inhibition of bacterial beta lactamase TEM-1 after 5 mins	Bacteria	1	ALA4364300	single protein format	Scientific Literature
9.	ALA4374955	B	Inhibition of bacterial N-terminal His-tagged TEV protease site linked TEM-1 (24 to 286 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay	Bacteria	35	ALA4373692	assay format	Scientific Literature
10.	ALA4416286	B	Binding affinity to bacterial wild type Beta-lactamase TEM-1 assessed as change in melting temperature at 100-fold molar excess compound concentration relative to apo-enzymes by SYPRO Orange dye based differential scanning fluorimetry	Bacteria	1	ALA4414607	single protein format	Scientific Literature
11.	ALA4416292	B	Inhibition of bacterial wild type Beta-lactamase TEM-1 pre-incubated for 5 mins before addition of chromogenic beta-lactamase substrate CENTA by spectrophotometry	Bacteria	3	ALA4414607	single protein format	Scientific Literature
12.	ALA4817144	B	Inhibition of bacterial Beta-lactamase TEM-1 (24 - 286) (unknown origin) expressed in Escherichia coli Transetta (DE3)	Bacteria	22	ALA4813997	assay format	Scientific Literature