#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3887059	B	Cell Free Inhibition Assay: The inhibitory concentrations (IC50, [uM]) of the beta -lactamase inhibitors against purified TEM-1, SHV-1 and AmpC beta -lactamases are assessed by determining the concentration of inhibitor at which 50% of the nitrocefin hydrolysis is inhibited by the particular enzyme. Assays are performed with beta -lactamases expressed in the pET system (Novagen, San Diego, Calif.) without signal peptides. They contain an N-terminal hexa-Histidine tag that is used for purification on Ni-NTA (Qiagen, Hilden, Germany). The compounds are prepared as 50 mM stocks in DMSO and diluted into buffer P1 (50 mM phosphate, pH 7) to a final concentration of 10% DMSO. All further dilutions are done in P2 (P1 with 10% DMSO). Enzyme and compound dilutions are pre-incubated for 10 min at 37 C. and the reaction is started with the addition of pre-warmed (37 C.) nitrocefin at a final concentration of 50 mM. The change in absorption at 490 nm is followed at 37 C. for 10 min with 30 s intervals.		42		assay format	BindingDB Database
2.	ALA4027949	B	Inhibition of recombinant N-terminal His-tagged bacterial Escherichia coli TEM-1 (24 to 286 residues) expressed in Escherichia coli BL21 (DE3) cells using FC5 as substrate preincubated up to 360 mins prior to substrate addition by fluorescence-based assay	Escherichia coli	2		cell-based format	Scientific Literature
3.	ALA4121762	B	Inhibition of recombinant N-terminal His-tagged Escherichia coli TEM1 (24 to 286 residues) expressed in Escherichia coli BL21(DE3) using FC5 as substrate assessed as residual activity at 200 uM	Escherichia coli	2		assay format	Scientific Literature
4.	ALA4121766	B	Inhibition of recombinant N-terminal His-tagged Escherichia coli TEM1 (24 to 286 residues) expressed in Escherichia coli BL21(DE3) at 200 uM using FC5 as substrate	Escherichia coli	57		assay format	Scientific Literature
5.	ALA4322458	B	Inhibition of recombinant bacterial class A serine beta-lactamase TEM-1 expressed in Escherichia coli assessed as reduction in breakdown of cephalosporin FC-5 preincubated for 10 mins followed by cephalosporin FC-5 addition by fluorescence method	Bacteria	1		assay format	Scientific Literature
6.	ALA4351600	B	Inhibition of bacterial beta-lactamase TEM-1	Bacteria	1		single protein format	Scientific Literature
7.	ALA4367635	B	Inhibition of bacterial beta lactamase TEM-1	Bacteria	1		single protein format	Scientific Literature
8.	ALA4367666	B	Inhibition of bacterial beta lactamase TEM-1 after 5 mins	Bacteria	1		single protein format	Scientific Literature
9.	ALA4374955	B	Inhibition of bacterial N-terminal His-tagged TEV protease site linked TEM-1 (24 to 286 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay	Bacteria	35		assay format	Scientific Literature
10.	ALA4416286	B	Binding affinity to bacterial wild type Beta-lactamase TEM-1 assessed as change in melting temperature at 100-fold molar excess compound concentration relative to apo-enzymes by SYPRO Orange dye based differential scanning fluorimetry	Bacteria	1		single protein format	Scientific Literature
11.	ALA4416292	B	Inhibition of bacterial wild type Beta-lactamase TEM-1 pre-incubated for 5 mins before addition of chromogenic beta-lactamase substrate CENTA by spectrophotometry	Bacteria	3		single protein format	Scientific Literature
12.	ALA4817144	B	Inhibition of bacterial Beta-lactamase TEM-1 (24 - 286) (unknown origin) expressed in Escherichia coli Transetta (DE3)	Bacteria	22		assay format	Scientific Literature