# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3223124 | B | Inhibition of erythrocyte 20S proteasome (unknown origin) assessed as inhibition of chymotrypsin activity after 15 mins using Suc-LLVY-AMC substrate by fluorescence assay | Homo sapiens | 1 | ALA3217720 | protein complex format | Scientific Literature | |
2. | ALA3223125 | B | Inhibition of erythrocyte 20S proteasome (unknown origin) assessed as inhibition of chymotrypsin activity at 50 uM after 15 mins using Suc-LLVY-AMC substrate by fluorescence assay | Homo sapiens | 1 | ALA3217720 | protein complex format | Scientific Literature | |
3. | ALA3223126 | B | Inhibition of erythrocyte 20S proteasome (unknown origin) assessed as inhibition of chymotrypsin activity at 10 uM after 15 mins using Suc-LLVY-AMC substrate by fluorescence assay | Homo sapiens | 1 | ALA3217720 | protein complex format | Scientific Literature | |
4. | ALA3223127 | B | Inhibition of 20S proteasome in human MelJuSo UbG76V-YFP cells assessed as accumulation of K48-linked poly-ubiquitinated proteins at 0.1 uM after 6 hrs by immunoblot method | Homo sapiens | 1 | ALA3217720 | cell-based format | Scientific Literature | |
5. | ALA3223128 | B | Inhibition of 20S proteasome in human MelJuSo UbG76V-YFP cells assessed as accumulation of UbG76V-YFP protein at 0.1 uM after 6 hrs by immunoblot method | Homo sapiens | 1 | ALA3217720 | cell-based format | Scientific Literature | |
6. | ALA3222879 | B | Inhibition of human 20S proteasome using Suc-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 90 mins by fluorescence assay | Homo sapiens | 3 | ALA3217678 | protein complex format | Scientific Literature | |
7. | ALA3222883 | B | Inhibition of human 20S proteasome using Suc-LLVY-AMC as substrate assessed as decrease in enzyme velocity | Homo sapiens | 2 | ALA3217678 | protein complex format | Scientific Literature | |
8. | ALA3222884 | B | Inhibition of 20S proteasome in human HeLa cells expressing GFP-tagged ubiquitin G76V mutant at 23 uM after 16 hrs by fluorescence assay | Homo sapiens | 2 | ALA3217678 | cell-based format | Scientific Literature | |
9. | ALA3222885 | B | Inhibition of 20S proteasome in human HeLa cells expressing GFP-tagged ubiquitin G76V mutant at 26 nM after 16 hrs by fluorescence assay | Homo sapiens | 1 | ALA3217678 | cell-based format | Scientific Literature | |
10. | ALA3240083 | B | Inhibition of chymotrypsin-like activity of human 20S proteasome using Suc-LLVY-AMC as substrate | Homo sapiens | 17 | ALA3232719 | protein complex format | Scientific Literature | |
11. | ALA3240084 | B | Inhibition of caspase-like activity of human 20S proteasome using Ac-nLPnLD-AMC as substrate | Homo sapiens | 13 | ALA3232719 | protein complex format | Scientific Literature | |
12. | ALA3240085 | B | Inhibition of trypsin-like activity of human 20S proteasome using Ac-RLR-AMC as substrate | Homo sapiens | 13 | ALA3232719 | protein complex format | Scientific Literature | |
13. | ALA3242187 | B | Reversible inhibition of chymotrypsin-like activity of human 20S proteasome using Suc-LLVY-AMC as substrate measured after drug wash-out | Homo sapiens | 2 | ALA3232719 | protein complex format | Scientific Literature | |
14. | ALA3242188 | B | Irreversible inhibition of chymotrypsin-like activity of human 20S proteasome using Suc-LLVY-AMC as substrate measured after drug wash-out | Homo sapiens | 2 | ALA3232719 | protein complex format | Scientific Literature | |
15. | ALA3266056 | B | Inhibition of chymotrypsin-like enzyme activity of purified human 20S proteasome using Suc-Leu-Leu-Val-Tyr-AMC as substrate at 1 ug/ml by fluorescence method | Homo sapiens | 33 | ALA3259806 | protein complex format | Scientific Literature | |
16. | ALA3266057 | B | Inhibition of chymotrypsin-like enzyme activity of purified human 20S proteasome using Suc-Leu-Leu-Val-Tyr-AMC as substrate by fluorescence method | Homo sapiens | 35 | ALA3259806 | protein complex format | Scientific Literature | |
17. | ALA3266564 | B | Inhibition of chymotrypsin-like activity of 26S proteasome in intact human Karpas 1106p cells assessed as substrate hydrolysis using Suc-LLVY-(D)-aminoluciferin as substrate after 3 hrs by cell-based Proteasome-Glo assay | Homo sapiens | 2 | ALA3259678 | cell-based format | Scientific Literature | |
18. | ALA3295791 | B | Inhibition of chymotrypsin-like of human 20S proteasome using chromophoric Suc-LLVY-AMC as substrate after 60 mins by fluorescence assay | Homo sapiens | 5 | ALA3286207 | protein complex format | Scientific Literature | |
19. | ALA4334249 | B | Inhibition of human 26S proteasome using suc-LLVY-AMC as substrate up to 40 mins by fluorescence assay | Homo sapiens | 2 | ALA4330170 | protein complex format | Scientific Literature | |
20. | ALA4370612 | B | Inhibition of 26S proteosome in human Hep3B cells grown under in hypoxic condition for 4 hrs assessed as downregulation of HIF1alpha level at 10 uM added together with cycloheximide and measured after up 45 mins post dose by Western blot analysis | Homo sapiens | 1 | ALA4368925 | cell-based format | Scientific Literature |