#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA616894	F	Percent of maximum stimulation of phosphatidylinositol turnover at cloned 5-hydroxytryptamine 2A receptor in 3T3 cells		3	ALA1127290	cell-based format	Scientific Literature
2.	ALA616896	F	The maximum responses of [3H]- inositol monophosphate accumulation in cells expressing the 5-hydroxytryptamine 2A receptor		3	ALA1127290	assay format	Scientific Literature
3.	ALA737877	F	Ability to antagonize head twitches in mice, observed after administration of (+/-)- DOI, was used to evaluate 5-HT2A receptor antagonistic property; Not determined	Mus musculus	1	ALA1137076	organism-based format	Scientific Literature
4.	ALA738482	F	The ability to antagonize head twitches in mice, observed after administration of (+/-)- DOI, was used to evaluate 5-HT2A receptor antagonistic property; Antagonist	Mus musculus	4	ALA1137076	organism-based format	Scientific Literature
5.	ALA738483	F	The ability to antagonize head twitches in mice, observed after administration of (+/-)- DOI, was used to evaluate 5-HT2A receptor antagonistic property; Not determined	Mus musculus	3	ALA1137076	organism-based format	Scientific Literature
6.	ALA738484	F	The ability to antagonize head twitches in mice, observed after administration of (+/-)- DOI, was used to evaluate 5-HT2A receptor antagonistic property; Not determined	Mus musculus	1	ALA1137076	organism-based format	Scientific Literature
7.	ALA738485	F	The ability to antagonize head twitches in mice, observed after administration of(+/-)- DOI, was used to evaluate 5-HT2A receptor antagonistic property; Not determined	Mus musculus	3	ALA1137076	organism-based format	Scientific Literature
8.	ALA2114839	F	PubChem BioAssay. Luminescence-based cell-based high throughput dose response assay for agonists of the mouse 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A). (Class of assay: confirmatory)	Mus musculus	237	ALA1201862	cell-based format	PubChem BioAssays
9.	ALA3887407	B	Radioligand Binding Assay: The detailed experimental protocols for the radioligand and functional receptor assays are available on the NIMH PDSP website at http://pdsp.med.unc.edu/UNC-CH %20Protocol %20Book.pdf. A. Serotonin receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 and 5-HT7. Assay Buffer: Standard Binding Buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, pH 7.4) Membrane Fraction Source: Transiently or stably transfected cell lines (e.g., HEK293, COS, CHO, NIH3T3). Protocol adapted from Roth et al. (1986), J. Pharmacol. Exp. Ther., 238(2): 480-485; and Roth et al. (1994), J. Pharmacol. Exp. Ther., 268(3): 1403-1410. Adrenergic Receptors: alpha1A, alpha1B, alpha2A, alpha2B, alpha2C, beta1, beta2, beta3 Assay Buffers: For alpha1 receptors, alpha1 Binding Buffer (20 mM Tris-HCl, 145 mM NaCl, pH 7.4); for alpha2 receptors, alpha2 Binding Buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.7); for beta receptors.		16	ALA3886311	assay format	BindingDB Database
10.	ALA4605031	F	Agonist activity at mouse 5-HT2A receptor expressed in Flp-In T-REx 293 cells assessed as induction of Gq-mediated calcium flux incubated for 1 hr by Flou4-AM dye based FLIPR assay	Mus musculus	3	ALA4602692	cell-based format	Scientific Literature
11.	ALA4605032	F	Agonist activity at mouse 5-HT2A receptor expressed in Flp-In T-REx 293 cells assessed as induction of Gq-mediated calcium flux incubated for 1 hr by Flou4-AM dye based FLIPR assay relative to serotonin	Mus musculus	2	ALA4602692	cell-based format	Scientific Literature