#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705046	Binding	Binding Assay: Binding assay using alpha 7 nAChR.	Homo sapiens	6	ALA3639286	single protein format	BindingDB Database
2.	ALA3706059	Binding	Binding Assay: The [3H]-methyllycaconitine binding assay is a modification of the method described by Davies et al. in Neuropharmacol. 1999, 38, 679-690.Rat brain tissue (hippocampus or whole brain) is homogenized in homogenization buffer (10% w/v, 0.32 M sucrose, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 0.01% (w/v) NaN3, pH 7.4, 4 C.) at 600 rpm in a glass homogenizer. The homogenate is centrifuged (1000xg, 4 C., 10 min) and the supernatant is removed. The pellet is resuspended (20% w/v) and the suspension is centrifuged (1000xg, 4 C., 10 min). The two supernatants are combined and centrifuged (15 000xg, 4 C., 30 min). The pellet obtained in this way is referred to as the P2 fraction.The P2 pellet is washed with binding buffer (50 mM Tris-HCl, 1 mM MgCl2, 120 mM NaCl, 5 mM KCl, 2 mM CaCl2, pH 7.4), and centrifuged (15 000x g, 4 C., 30 min), twice.The P2 membranes are resuspended in binding buffer and incubated in a volume of 250 ul (amount of membrane protein 0.1-0.5 mg).	Homo sapiens	21	ALA3639090	tissue-based format	BindingDB Database
3.	ALA3751564	Binding	Agonist activity at human alpha7 nAChR expressed in xenopus oocytes assessed as induction of PAM-sensitive desensitization at 30 uM by two-electrode voltage clamp electrophysiology relative to acetylcholine	Homo sapiens	22	ALA3745606	cell-based format	Scientific Literature
4.	ALA3751565	Binding	Agonist activity at human alpha7 nAChR expressed in xenopus oocytes assessed as potentiation of PNU-120596-induced PAM-sensitive desensitization at 30 uM by two-electrode voltage clamp electrophysiology relative to acetylcholine	Homo sapiens	22	ALA3745606	cell-based format	Scientific Literature
5.	ALA3751566	Binding	Partial agonist activity at human alpha7 nAChR expressed in xenopus oocytes assessed as potentiation of PNU-120596-induced PAM-sensitive desensitization at 30 uM by two-electrode voltage clamp electrophysiology relative to acetylcholine	Homo sapiens	6	ALA3745606	cell-based format	Scientific Literature
6.	ALA3751567	Binding	Partial agonist activity at human alpha7 nAChR expressed in xenopus oocytes assessed as induction of PAM-sensitive desensitization at 30 uM by two-electrode voltage clamp electrophysiology relative to acetylcholine	Homo sapiens	6	ALA3745606	cell-based format	Scientific Literature
7.	ALA3751571	Binding	Partial agonist activity at human alpha7 nAChR expressed in xenopus oocytes assessed as induction of PAM-sensitive desensitization by measuring ratio of peak current to net charge at 30 uM by two-electrode voltage clamp electrophysiology relative to acetylcholine	Homo sapiens	6	ALA3745606	cell-based format	Scientific Literature
8.	ALA3761531	Binding	Displacement of [125I] alpha bungarotoxin from human alpha7 nACh receptor incubated for 20 secs	Homo sapiens	1	ALA3758078	single protein format	Scientific Literature
9.	ALA3761536	Binding	Agonist activity at human alpha7 nACh receptor expressed in rat GH4C1 cells assessed as increase in current amplitudes after 2 to 3 days by patch clamp assay	Homo sapiens	1	ALA3758078	cell-based format	Scientific Literature
10.	ALA3761540	Binding	Agonist activity at human alpha7 nACh receptor expressed in rat GH4C1 cells assessed as increase in current amplitudes at 0.1 to 100 uM after 2 to 3 days by patch clamp assay	Homo sapiens	1	ALA3758078	cell-based format	Scientific Literature
11.	ALA3766467	Binding	Agonist activity at human alpha7 receptor expressed in mouse Neuro2a cells assessed as induction of intracellular calcium level preincubated with PNU-120596 for 20 mins followed by compound addition measured every 2 sec for 3 mins by fluorescence microplate reader analysis	Homo sapiens	4	ALA3763033	cell-based format	Scientific Literature
12.	ALA3767210	Binding	Antagonist activity at human alpha7 receptor expressed in mouse Neuro2a cells assessed as inhibition of acetylcholine iodide-induced intracellular calcium level at 300 uM preincubated with PNU-120596 for 20 mins followed by acetylcholine iodide addition along with compound treatment measured every 2 sec for 3 mins by fluorescence microplate reader analysis	Homo sapiens	1	ALA3763033	cell-based format	Scientific Literature
13.	ALA3767211	Binding	Agonist activity at human alpha7 receptor expressed in mouse Neuro2a cells assessed as induction of intracellular calcium level measured every 2 sec for 3 mins by fluorescence microplate reader analysis in presence of 2 uM alpha7 receptor antagonist alpha-cobratoxin	Homo sapiens	3	ALA3763033	cell-based format	Scientific Literature
14.	ALA3767212	Binding	Antagonist activity at human alpha7 receptor expressed in mouse Neuro2a cells assessed as inhibition of acetylcholine iodide-induced intracellular calcium level at 50 uM preincubated with PNU-120596 for 20 mins followed by acetylcholine iodide addition along with compound treatment measured every 2 sec for 3 mins by fluorescence microplate reader analysis	Homo sapiens	1	ALA3763033	cell-based format	Scientific Literature
15.	ALA3788097	Binding	Agonist activity at human alpha7 nAChR expressed in xenopus oocytes preincubated for 10 mins followed by acetylcholine addition by patch clamp electrophysiological assay	Homo sapiens	48	ALA3785087	cell-based format	Scientific Literature
16.	ALA3788098	Binding	Binding affinity to human alpha7 nAChR expressed in xenopus oocytes preincubated for 10 mins followed by acetylcholine addition by patch clamp electrophysiological assay relative to acetylcholine	Homo sapiens	48	ALA3785087	cell-based format	Scientific Literature
17.	ALA3788099	Binding	Displacement of [3H]-MLA from human alpha7 nAChR expressed in xenopus oocytes in presence of alpha7 nAChR positive allosteric modulator PNU-120596	Homo sapiens	1	ALA3785087	cell-based format	Scientific Literature
18.	ALA3788335	Binding	Displacement of [3H]-MLA from human alpha7 nAChR expressed in xenopus oocytes	Homo sapiens	1	ALA3785087	cell-based format	Scientific Literature
19.	ALA3810515	Binding	Agonist activity at alpha7 nAChR (unknown origin)	Homo sapiens	5	ALA3808359	single protein format	Scientific Literature
20.	ALA824052	Functional	Effective concentration that causes inhibition of alpha-7 nAChR, was determined. Values are expressed as EC50 +/- SEM.		5	ALA1134160	assay format	Scientific Literature