| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3049461 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as Stern-Volmer quenching constant at 47 degC by fluorescence spectroscopy | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 2. | ALA3049462 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as Stern-Volmer quenching constant at 37 degC by fluorescence spectroscopy | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 3. | ALA3049463 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as Stern-Volmer quenching constant at 27 degC by fluorescence spectroscopy | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 4. | ALA3049464 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) at 20 to 200 uM at 27 to 47 degC after 30 min by fluorescence quenching assay | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 5. | ALA3049465 | B | Inhibition of C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as reduction in initial velocity at the step of threonine activation preincubated for 10 min with 4 uM enzyme before threonine addition measured after 30 min by HPLC analysis | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 6. | ALA3048941 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as decrease in beta sheet content of the protein at protein to compound ratio of 1:5 by CD spectroscopy (Rvb = 25.6%) | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 7. | ALA3049235 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as decrease in beta sheet content of the protein at protein to compound ratio of 1:1 by CD spectroscopy (Rvb = 25.6%) | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 8. | ALA3049236 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as increase in alpha helicity of the protein at protein to compound ratio of 1:5 by CD spectroscopy (Rvb = 18.3%) | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 9. | ALA3049237 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as increase in alpha helicity of the protein at protein to compound ratio of 1:1 by CD spectroscopy (Rvb = 18.3%) | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 10. | ALA3049238 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as static fluorescence quenching by FRET analysis | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 11. | ALA3049239 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as positive entropy and enthalpy changes by themodynamic analysis | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 12. | ALA3049240 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as equilibrium binding constant by fluorescence spectroscopy | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 13. | ALA3049241 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as quenching rate constant at 47 degC by fluorescence spectroscopy | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 14. | ALA3049242 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as quenching rate constant at 37 degC by fluorescence spectroscopy | Phytophthora sojae | 1 | assay format | Scientific Literature | ||
| 15. | ALA3049243 | B | Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as quenching rate constant at 27 degC by fluorescence spectroscopy | Phytophthora sojae | 1 | assay format | Scientific Literature |
The page will load shortly, Thanks for your patience!