#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA866099	B	Inhibition of Cryptosporidium hominis DHFR	Cryptosporidium hominis	7		single protein format	Scientific Literature
2.	ALA3056823	B	Ratio of trimethorpin IC50 to compound IC50 for Cryptosporidium parvum recombinant DHFR	Cryptosporidium parvum	4		single protein format	Scientific Literature
3.	ALA3056825	B	Inhibition of Cryptosporidium parvum recombinant DHFR using dihydrofolate as substrate incubated for 5 min prior to substrate addition by spectrophotometric assay	Cryptosporidium parvum	12		single protein format	Scientific Literature
4.	ALA3136074	B	Stabilization of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase assessed as resistance to enzyme sensitivity to trypsin proteolysis at 100 uM by SDS-PAGE mthod	Cryptosporidium hominis	1		single protein format	Scientific Literature
5.	ALA3136075	B	Destabilization of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase assessed as increase in enzyme sensitivity to trypsin proteolysis at 750 uM by SDS-PAGE mthod	Cryptosporidium hominis	1		single protein format	Scientific Literature
6.	ALA3136076	B	Destabilization of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase assessed as increase in enzyme sensitivity to trypsin proteolysis at 2000 uM by SDS-PAGE mthod	Cryptosporidium hominis	1		single protein format	Scientific Literature
7.	ALA3136078	B	Inhibition of thymidylate synthase activity of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase by steady state kinetic assay	Cryptosporidium hominis	1		single protein format	Scientific Literature
8.	ALA3136081	B	Noncompetitive inhibition of DHFR activity of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase by Lineweaver-Burk plot	Cryptosporidium hominis	2		single protein format	Scientific Literature
9.	ALA3136082	B	Noncompetitive inhibition of DHFR activity of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase assessed as effect on enzyme Km using NADPH substrate by steady state kinetic assay	Cryptosporidium hominis	2		single protein format	Scientific Literature
10.	ALA3136083	B	Noncompetitive inhibition of DHFR activity of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase assessed as reduction in enzyme Vmax using NADPH substrate by steady state kinetic assay	Cryptosporidium hominis	2		single protein format	Scientific Literature
11.	ALA3136084	B	Noncompetitive mixed type inhibition of DHFR activity of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase assessed as increase in enzyme Km using H2folate substrate by steady state kinetic assay	Cryptosporidium hominis	2		single protein format	Scientific Literature
12.	ALA3136203	B	Noncompetitive mixed type inhibition of DHFR activity of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase assessed as reduction in enzyme Vmax using H2folate substrate by steady state kinetic assay	Cryptosporidium hominis	2		single protein format	Scientific Literature
13.	ALA3136204	B	Inhibition of DHFR activity of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase by steady state kinetic assay	Cryptosporidium hominis	2		single protein format	Scientific Literature
14.	ALA3364301	B	Inhibition of thymidylate synthase activity of Cryptosporidium hominis bifunctional dihydrofolate reductase-thymidylate synthase assessed as reduction in conversion of dUMP and CH2H4F to products dTMP and H2F	Cryptosporidium hominis	1		single protein format	Scientific Literature
15.	ALA3364304	B	Inhibition of DHFR activity of Cryptosporidium hominis bifunctional dihydrofolate reductase-thymidylate synthase assessed as reduction in NADPH oxidation to NAD	Cryptosporidium hominis	1		single protein format	Scientific Literature
16.	ALA3705793	B	Spectrophotometric Enzyme Assay: Compounds were evaluated in spectrophotometric enzyme assays using ChDHFR-TS and hDHFR. Inhibition constants (IC50) were measured (see Table 4). The lead compound, X, has an inhibition constant of 38 nM and modest selectivity (8-fold). All of the biphenyl compounds are more potent than the initial lead compound X and exhibit greater selectivity for the pathogenic enzyme. The most potent racemic compound, D(rac), a 5′-biphenyl derivative, is also the most selective of the racemic compounds (944-fold). The single R enantiomer of this 5′-biphenyl analog is the most potent (1.1 nM) and most selective (1273-fold) of all known compounds tested against the Cryptosporidium DHFR enzyme.	Cryptosporidium hominis	11		single protein format	BindingDB Database