# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA668916 | B | In vitro inhibition against hog kidney diamine oxidase | Sus scrofa | 1 | single protein format | Scientific Literature | ||
2. | ALA668917 | B | Tested for ability to competitively inhibit hog kidney diamine oxidase (DAO) in a spectrometric assay | Sus scrofa | 5 | single protein format | Scientific Literature | ||
3. | ALA668918 | B | Inhibitory activity against Swine kidney diamine oxidase (SKDAO) | Sus scrofa | 4 | single protein format | Scientific Literature | ||
4. | ALA668919 | B | Inhibitory activity against Swine kidney diamine oxidase (SKDAO); No inhibition, corresponding to value >10e-4 | Sus scrofa | 2 | single protein format | Scientific Literature | ||
5. | ALA827081 | B | Km for Diamine oxidase of porcine kidney | Sus scrofa | 2 | single protein format | Scientific Literature | ||
6. | ALA828103 | B | Inhibition of putrescine binding to Diamine oxidase of porcine kidney | Sus scrofa | 1 | single protein format | Scientific Literature | ||
7. | ALA828122 | B | Inhibition of putrescine binding to against Diamine oxidase of porcine kidney | Sus scrofa | 21 | single protein format | Scientific Literature | ||
8. | ALA828620 | B | Vmax for Diamine oxidase of porcine kidney | Sus scrofa | 2 | single protein format | Scientific Literature | ||
9. | ALA874951 | F | Inhibition of putrescine binding to Diamine oxidase of porcine kidney | Sus scrofa | 1 | assay format | Scientific Literature | ||
10. | ALA3242684 | B | Mixed type inhibition of recombinant human DAO expressed in drosophila S2 cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
11. | ALA3888274 | B | DAO Inhibition Assay: Recombinant human DAO (2.4 μg/mL) was used as source of DAO enzyme activities. The assay was performed as described in the method for human SSAO/VAP-1 (Example 5) except the substrate used was 200 μM putrescine, and the control wells contained 10 μM aminoguanidine instead of Mofegiline: Briefly, in a standard 96 well plate assay 50 μL of purified human SSAO/VAP-1 (0.25 μg/mL) in 0.1 M NaPO4 buffer (pH 7.4) was added into each well. Test compounds were dissolved in DMSO and tested in a Concentration Response Curve (CRC) with 4-9 data points, typically in the micromolar or nanomolar range after incubation with human SSAO/VAP-1 for 30 min at 37 °C. After 30 min incubation, 50 μL of the reaction mixture containing 600 μM benzylamine (Sigma Aldrich), 120 μM Amplex Red (Sigma Aldrich) and 1.5 U/mL horseradish peroxidase (Sigma Aldrich) prepared in 0.1 M NaPO4 buffer (pH 7.4) were added to the corresponding well. The fluorescence unit (RFU) was read every 2.5 min for 30 min at 37 °C. excitation 565 nm and emission 590 (Optima; BMG labtech). The slope of the kinetics for each well was calculated using MARS data analysis software (BMG labtech) and this value was used to deduce the IC50 value (Dotmatics). | 24 | single protein format | BindingDB Database | |||
12. | ALA4052339 | B | Inhibition of recombinant human DAO assessed as reduction of H2O2 production from oxidative deamination of DAP at 10 uM preincubated for 2 hrs followed by substrate addition measured every 2 mins for 1 hr by amplex red reagent-based fluorescence assay | Homo sapiens | 3 | single protein format | Scientific Literature |