#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1212436	F	Antagonist activity at human alpha4 nAChR in human IMR32 cells	Homo sapiens	1	ALA1208721	cell-based format	Scientific Literature
2.	ALA3376389	B	Inhibition of alpha4 nAChR (unknown origin)	Homo sapiens	1	ALA3352721	single protein format	Scientific Literature
3.	ALA3707774	B	Radioligand Binding: The binding of [3H]MLA was measured using a modification of the methods of Davies et al., Neuropharmacol. 38: 679 (1999). [3H]MLA (Specific Activity=25-35 Ci/mmol) was obtained from Tocris. The binding of [3H]MLA was determined using a 2 h incubation at 21 C. Incubations were conducted in 48-well micro-titre plates and contained about 200 μg of protein per well in a final incubation volume of 300 uL. The incubation buffer was PBS and the final concentration of [3H]MLA was 5 nM. The binding reaction was terminated by filtration of the protein containing bound ligand onto glass fiber filters (GF/B, Brandel) using a Brandel Tissue Harvester at room temperature. Filters were soaked in de-ionized water containing 0.33% polyethyleneimine to reduce non-specific binding. Each filter was washed with PBS (3x1 mL) at room temperature. Non-specific binding was determined by inclusion of 50 uM non-radioactive MLA in selected wells.	Homo sapiens	79	ALA3638474	single protein format	BindingDB Database
4.	ALA4887021	B	N (muscle-type) (h) CEREP ligand profiling	Homo sapiens	1	ALA4507298	single protein format	Donated Chemical Probes - SGC Frankfurt
5.	ALA5291770	B	Binding affinity towards human CHRNA4 in an in vitro assay with cellular components measured by scintillation proximity assay		1,170	ALA5291721	single protein format	Scientific Literature