# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1212436 | F | Antagonist activity at human alpha4 nAChR in human IMR32 cells | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
2. | ALA3376389 | B | Inhibition of alpha4 nAChR (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
3. | ALA3707774 | B | Radioligand Binding: The binding of [3H]MLA was measured using a modification of the methods of Davies et al., Neuropharmacol. 38: 679 (1999). [3H]MLA (Specific Activity=25-35 Ci/mmol) was obtained from Tocris. The binding of [3H]MLA was determined using a 2 h incubation at 21 C. Incubations were conducted in 48-well micro-titre plates and contained about 200 μg of protein per well in a final incubation volume of 300 uL. The incubation buffer was PBS and the final concentration of [3H]MLA was 5 nM. The binding reaction was terminated by filtration of the protein containing bound ligand onto glass fiber filters (GF/B, Brandel) using a Brandel Tissue Harvester at room temperature. Filters were soaked in de-ionized water containing 0.33% polyethyleneimine to reduce non-specific binding. Each filter was washed with PBS (3x1 mL) at room temperature. Non-specific binding was determined by inclusion of 50 uM non-radioactive MLA in selected wells. | Homo sapiens | 79 | single protein format | BindingDB Database | ||
4. | ALA4887021 | B | N (muscle-type) (h) CEREP ligand profiling | Homo sapiens | 1 | single protein format | Donated Chemical Probes - SGC Frankfurt | ||
5. | ALA5291770 | B | Binding affinity towards human CHRNA4 in an in vitro assay with cellular components measured by scintillation proximity assay | 1,170 | single protein format | Scientific Literature |