#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705635	Binding	Enzyme Inhibition Assay: The enzyme inhibition assays used monitored the ability of a test compound to bind and prevent the hydrolysis of a fluorogenic substrate in a concentration-dependent mariner. Specifically, the enzyme activity of recombinant human GCase (rhGCase; Cerezyme, Genzyme Corp.) was measured using the 4-methylumbelliferyl-β-D-glucopyranoside (4-MU-β-D-Glc) fluorogenic substrate in the absence or in the presence of varying amounts of each test compound.	Homo sapiens	7	ALA3639164	single protein format	BindingDB Database
2.	ALA3705812	Binding	Enzyme Assay: To 2.5 μl of supernatant (in 96-well plates) was added 17.5 μl reaction buffer (citrate phosphate buffer, pH 4.5, no Triton X-100), and 50 μl of 4-methyl umbelliferone (4-MU)-labeled substrate, β-glucopyranoside, or a labeled negative controls (α-glucopyranoside or α-galacatopyranoside). Plates were incubated at 37° for 1 hour, followed by the addition of 70 μl stop buffer (0.4 M glycine-NaOH, pH 10.6). Activity of GCase was determined by measuring the emission at 460 nm by exciting at 355 nm using a 1 second read time per well (Victor2 multilabel counter-Wallac) Enzyme activity was normalized to the amount in μl of lysate added, and enzyme activity per μl of lysate was estimated.		20	ALA3639145	assay format	BindingDB Database
3.	ALA3705881	Binding	Enzyme Activity Assay: Activity of GBA was measured at 37° C. with 4-methylumbelliferyl β-D-glucopyranoside as substrate as reported previously. To determine the IC50 value, the inhibitors were preincubated for 30 min with the enzyme before addition of the substrate mixture. The incubation mixture contained 3 mM fluorogenic substrate, 0.2% (w/v) sodium taurocholate and 0.1% (v/v) Triton X-100 in 150 mM McIlvaine buffer, pH 5.2. After stopping the incubation with excess NaOH-glycine (pH 10.3), we measured fluorescence with a fluorimeter LS 30 (Perkin Elmer) using λex 366 nm and λem 445 nm. Activity of lactase was quantified by measuring liberated glucose from lactase38. In vivo activity of GBA in cells was measured using FDG as substrate and FACS32.	Homo sapiens	4	ALA3638447	organism-based format	BindingDB Database
4.	ALA3737684	Binding	Inhibition of human beta-glucosidase isolated from human fibroblasts incubated for 30 mins using 4-methylumbelliferone -conugated beta-D-galactoside as substrate by fluorescence analysis	Homo sapiens	16	ALA3734673	single protein format	Scientific Literature
5.	ALA3788464	Binding	Pharmacological chaperone activity at beta-glucocerebrosidase N370S mutant in Gaucher disease patient-derived lymphoblasts with GCase N370S mutant assessed as increase in enzyme activity at 2.5 uM after 3 days by fluorimetric analysis relative to control	Homo sapiens	2	ALA3784998	single protein format	Scientific Literature
6.	ALA3788465	Binding	Pharmacological chaperone activity at beta-glucocerebrosidase L444P mutant in Gaucher disease patient-derived lymphoblasts expressing with GCase L444P mutant assessed as increase in enzyme activity at 5 uM after 3 days by fluorimetric analysis relative to control	Homo sapiens	2	ALA3784998	single protein format	Scientific Literature
7.	ALA3788467	Binding	Pharmacological chaperone activity at beta-glucocerebrosidase N370S mutant in Gaucher disease patient-derived lymphoblasts with GCase N370S mutant assessed as increase in enzyme activity at 10 uM after 3 days by fluorimetric analysis relative to control	Homo sapiens	1	ALA3784998	single protein format	Scientific Literature
8.	ALA3788471	Binding	Inhibition of human recombinant imiglucerase using 4-MU-beta-D-Glu as substrate at pH 5.2 after 30 mins by fluorometric analysis	Homo sapiens	14	ALA3784998	single protein format	Scientific Literature
9.	ALA3788472	Binding	Inhibition of human recombinant imiglucerase using 4-MU-beta-D-Glu as substrate at pH 7.0 after 30 mins by fluorometric analysis	Homo sapiens	14	ALA3784998	single protein format	Scientific Literature
10.	ALA3788473	Binding	Competitive inhibition of human recombinant imiglucerase using 4-MU-beta-D-Glu as substrate at pH 5.2 after 30 mins by fluorometry based Lineweaver-Burk plot analysis	Homo sapiens	14	ALA3784998	single protein format	Scientific Literature
11.	ALA3788474	Binding	Pharmacological chaperone activity at beta-glucocerebrosidase N370S mutant in Gaucher disease patient-derived lymphoblasts with GCase N370S mutant assessed as increase in enzyme activity at 3.125 to 100 uM after 3 days by fluorimetric analysis relative to control	Homo sapiens	14	ALA3784998	single protein format	Scientific Literature
12.	ALA3790451	Binding	Pharmacological chaperone activity at beta-glucocerebrosidase N370S mutant in Gaucher disease patient-derived lymphoblasts with GCase N370S mutant assessed as increase in enzyme activity at 12.5 uM after 3 days by fluorimetric analysis relative to control	Homo sapiens	2	ALA3784998	single protein format	Scientific Literature
13.	ALA3790452	Binding	Pharmacological chaperone activity at beta-glucocerebrosidase N370S mutant in Gaucher disease patient-derived lymphoblasts with GCase N370S mutant assessed as increase in enzyme activity at 50 uM after 3 days by fluorimetric analysis relative to control	Homo sapiens	2	ALA3784998	single protein format	Scientific Literature
14.	ALA642871	Binding	Competitive Inhibition constant on almonds beta Glucosidase at pH 5.0	Prunus dulcis	1	ALA1150516	single protein format	Scientific Literature
15.	ALA651771	Binding	Compound was evaluated for its binding affinity towards E-coli Beta-galactosidase	Escherichia coli	1	ALA1125856	single protein format	Scientific Literature
16.	ALA650036	Binding	Inhibitory activity against Agrobacterium sp. Beta-glucosidase employing fluorescence spectrometric method	Agrobacterium	5	ALA1134071	single protein format	Scientific Literature
17.	ALA650037	Binding	Tested for competitive inhibitory activity against beta-Glucosidase	Agrobacterium	6	ALA1128872	single protein format	Scientific Literature
18.	ALA650038	Binding	Tested for inhibitory activity against Beta-glucosidase from Agrobacterium species	Agrobacterium	7	ALA1134733	single protein format	Scientific Literature
19.	ALA650051	Binding	Inhibitory activity against Beta-glucosidase of almonds at 1 mM; No inhibition	Prunus dulcis	1	ALA1133967	single protein format	Scientific Literature
20.	ALA871892	Binding	Inhibitory activity against Beta-glucosidase of Caldocellum saccharolyticum at 1 mM; No inhibition	Caldicellulosiruptor saccharolyticus	9	ALA1133967	single protein format	Scientific Literature