#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA766951	B	The compound was tested in vitro for the inhibitory activity against Protein tyrosine phosphatase PEST (SH-PTP1) (human PTPases.)		2		single protein format	Scientific Literature
2.	ALA2161836	B	Inhibition of PTP-PEST expressed in Escherichia coli BL21 using pNPP substrate		12		assay format	Scientific Literature
3.	ALA1738078	F	PUBCHEM_BIOASSAY: SAR Selectivity Analysis of small molecule inhibitors of PEST using pCAP in a fluorescence assay. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1779, AID1784, AID2135]	Homo sapiens	81		assay format	PubChem BioAssays
4.	ALA2401195	B	Inhibition of recombinant PTP-PEST (unknown origin) using pNPP as substrate by spectrophotometric analysis	Homo sapiens	2		single protein format	Scientific Literature
5.	ALA3888478	B	Enzyme Kinetic Assay: PTP activity was assayed using p-nitrophenyl phosphate (pNPP) as a substrate in 3,3-dimethylglutarate buffer (50 mM 3,3-dimethylglutarate, pH 7.0, 1 mM EDTA, 150 mM NaCl, 2 mM DTT, 0.1 mg/mL BSA) at 25° C. The assays were performed in 96-well plates. Normally, to determine the IC50 values, the reaction was initiated by the addition of enzyme (final concentration at 10 nM) to a reaction mixture (0.2 mL) containing 2 mM (Km for the substrate) pNPP with various concentrations of inhibitors. The reaction rate was measured using a SpectraMax Plus 384 Microplate Spectrophotometer (Molecular Devices). To determine the mode of inhibition, the reactions were initiated by the addition of PTP-MEG2 to the reaction mixtures (0.2 mL) containing various concentrations of pNPP with different concentrations of the inhibitor 7.		1		single protein format	BindingDB Database
6.	ALA4253922	B	Inhibition of PTP-PEST (unknown origin) using p-nitrophenyl phosphate as substrate preincubated for 10 mins followed by substrate addition measured every minute for 10 mins by UV-VIS spectrophotometric analysis	Homo sapiens	8		single protein format	Scientific Literature
7.	ALA4265153	B	Inhibition of recombinant human PTP-PEST using p-nitrophenyl phosphate as substrate preincubated for 10 mins followed by substrate addition and measured every minute for 10 mins by UV-VIS spectrophotometric analysis	Homo sapiens	4		single protein format	Scientific Literature
8.	ALA4417876	B	Inhibition of PTPN12 (unknown origin) assessed as change in enzyme activity at 150 uM using tyrosine phosphopeptide-2 substrate incubated for 15 mins relative to control	Homo sapiens	1		single protein format	Patent Bioactivity Data
9.	ALA4625402	A	Inhibition of PTP-PEST (unknown origin)	Homo sapiens	1		single protein format	Scientific Literature
10.	ALA4625407	A	Competitive inhibition of PTP-PEST (unknown origin) assessed as inhibition constant using varying level of pNPP as substrate by Lineweaver-Burk plot analysis	Homo sapiens	1		single protein format	Scientific Literature
11.	ALA4625419	A	Reversible inhibition of PTP-PEST (unknown origin) assessed as enzyme recovery activity by dilution assay relative to control	Homo sapiens	1		single protein format	Scientific Literature
12.	ALA4626308	A	Inhibition of PTP-PEST (unknown origin) expressed in Escherichia coli BL21 using p-nitrophenyl phosphate as substrate measured after 30 mins by UV-vis spectrophotometric method	Homo sapiens	3		assay format	Scientific Literature
13.	ALA4712048	B	Inhibition of PTPN12 (unknown origin) pre-incubated for 20 mins followed by fluorescence substrate addition and measured after 120 mins by DiFMUP assay	Homo sapiens	1		single protein format	Scientific Literature
14.	ALA4719015	B	Inhibition of His-tagged PTP-PEST (unknown origin) expressed in Escherichia coli BL21 cells using para-nitrophenyl phosphate as substrate incubated for 5 to 10 mins by microplate reader based spectrophotometric analysis	Homo sapiens	9		cell-based format	Scientific Literature
15.	ALA5217154	B	Inhibition of PEST(unknown origin)	Homo sapiens	3		single protein format	Scientific Literature