# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2384495 | B | Inhibition of mouse EL expressed in HEK293 cells using PED-A1 as substrate by spectrophotometry | Mus musculus | 3 | ALA2380223 | cell-based format | Scientific Literature | |
2. | ALA3095694 | B | Inhibition of mouse endothelial lipase using PLAi as substrate measured for 30 mins by cell-based fluorescence assay | Mus musculus | 4 | ALA3091422 | cell-based format | Scientific Literature | |
3. | ALA3887785 | B | Endothelial Lipase Assay: To assay for cell surface lipase activity, cells expressing human endothelial lipase (EL) or LPL were plated in CellBIND® 384-well plates (Corning, Lowell, Mass.) in 25 μL serum free medium at a density of 2000 cells/well. After 18-24 hours incubation at 37° C., the medium was removed and replaced with 15 μL assay buffer [Hank's Buffered Saline Solution with 25 mM HEPES pH 7.2] and 15 μL PLA1 substrate for a final concentration of 10 μM using a Multidrop reagent dispenser. Fluorescence signal was monitored for 30 min at 37° C. on a Safire II plate reader in kinetic mode (60 cycles, kinetic interval: 30 seconds) with an excitation wavelength of 490 nm and an emission wavelength of 515 nm. | 27 | ALA3886450 | cell-based format | BindingDB Database | ||
4. | ALA4199699 | B | Inhibition of recombinant mouse endothelial lipase expressed in HEK293F cells using HDL as substrate pretreated for 10 mins followed by substrate addition and measured after 30 mins by LC/MS/MS analysis | Mus musculus | 1 | ALA4196117 | cell-based format | Scientific Literature | |
5. | ALA4199701 | B | Competitive inhibition of recombinant mouse endothelial lipase expressed in HEK293F cells using varying levels of HDL as substrate pretreated for 10 mins followed by substrate addition and measured after 30 mins by LC/MS/MS analysis | Mus musculus | 1 | ALA4196117 | cell-based format | Scientific Literature | |
6. | ALA4232362 | B | Inhibition of endothelial lipase in mouse cells after 30 mins by fluorescence assay | Mus musculus | 1 | ALA4229386 | cell-based format | Scientific Literature | |
7. | ALA4325774 | B | Inhibition of endothelial lipase in hepatic lipase knock-out mouse plasma | Mus musculus | 7 | ALA4321883 | cell-free format | Scientific Literature | |
8. | ALA4390141 | B | Inhibition of mouse endothelial lipase expressed in HEK293F cells using D31-POPC-HDL as substrate incubated for 2 hrs in presence of mouse plasma by LC/MS analysis | Mus musculus | 8 | ALA4387752 | cell-based format | Scientific Literature | |
9. | ALA4390152 | B | Inhibition of endothelial lipase in hepatic lipase knock-out mouse plasma using D31-POPC-HDL as substrate incubated for 2 hrs by LC/MS analysis | Mus musculus | 1 | ALA4387752 | cell-free format | Scientific Literature | |
10. | ALA4390153 | B | Inhibition of hepatic lipase in endothelial lipase knock-out mouse plasma using D31-POPC-HDL as substrate incubated for 2 hrs by LC/MS analysis | Mus musculus | 1 | ALA4387752 | cell-free format | Scientific Literature |