| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3405048 | B | Inhibition of human Orai1/STIM1 expressed in HEK293 cells assessed as inhibition of channel-mediated calcium current by electrophysiology assay in presence of 10 mM EGTA | Homo sapiens | 3 | ALA3399948 | cell-based format | Scientific Literature | |
| 2. | ALA3706248 | B | Inhibition Assay: Inhibition of CRAC channels was determined following thapsigargin (Sigma, Cat # T9033) induced endoplasmic calcium release in Jurkat cells. (see Yasurio Yonetoky et. al Bio. & Med. Chem. 14 (2006) 4750-4760). Cells were centrifuged and resuspended in equal volumes f Ca2+ and Mg2+ free Hanks buffer and Fluo-8 NW dye (ABD Bioquest, Inc., Sunnyvale, Calif.) loading solution at 2x105 cells/100 ul/well in 96-well black plate. Plate is incubated at 37 C./5% CO2 for 30 mM followed by further 15 mM incubation at room temperature. Test compounds (DMSO stocks diluted in Ca2+ and Mg2+ free Hanks buffer) at desired concentrations were added to the wells and incubated for 15 mM. Thapsigargin (1 uM final concentration) was added to the wells and incubated for 15 min to inhibit the Sarco-endoplasmic reticulum Ca2+ ATPase pump thereby depleting endoplasmic calcium and raising cytosolic calcium concentrations. Store-operated calcium entry was initiated by adding extracellular Ca2+. | 10 | ALA3639112 | cell-based format | BindingDB Database | ||
| 3. | ALA3870005 | B | Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction of SERCA inhibition-induced ER release preincubated for 15 mins followed by CPA addition by PBX-based FLIPR assay | Homo sapiens | 5 | ALA3868625 | cell-based format | Scientific Literature | |
| 4. | ALA3870006 | B | Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction of SERCA inhibition-induced ER release at 100 uM preincubated for 15 mins followed by CPA addition by PBX-based FLIPR assay | Homo sapiens | 16 | ALA3868625 | cell-based format | Scientific Literature | |
| 5. | ALA3870007 | B | Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction in BAPTA-induced Ca2+ depletion-stimulated SOCE activity preincubated for 15 mins followed by BAPTA addition in presence of extracellular Ca2+ by PBX-based FLIPR assay | Homo sapiens | 11 | ALA3868625 | cell-based format | Scientific Literature | |
| 6. | ALA3870008 | B | Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction in BAPTA-induced Ca2+ depletion-stimulated SOCE activity at 100 uM preincubated for 15 mins followed by BAPTA addition in presence of extracellular Ca2+ by PBX-based FLIPR assay relative to control | Homo sapiens | 11 | ALA3868625 | cell-based format | Scientific Literature | |
| 7. | ALA3870009 | B | Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction in BAPTA-induced Ca2+ depletion-stimulated SOCE activity at 30 uM preincubated for 15 mins followed by BAPTA addition in presence of extracellular Ca2+ by PBX-based FLIPR assay relative to control | Homo sapiens | 1 | ALA3868625 | cell-based format | Scientific Literature | |
| 8. | ALA3870014 | B | Inhibition of Orai1 (unknown origin) by patch clamp assay | Homo sapiens | 1 | ALA3868625 | assay format | Scientific Literature | |
| 9. | ALA4123114 | B | Inhibition of BAPTA-induced Orai1-medited store operated calcium entry in human MDA-MB-231 cells pretreated for 15 mins followed by BAPTA addition by PBX dye based FLIPR assay | Homo sapiens | 27 | ALA4118190 | cell-based format | Scientific Literature | |
| 10. | ALA4123116 | B | Inhibition of BAPTA-induced Orai1-medited store operated calcium entry in human MDA-MB-231 cells at 30 uM pretreated for 15 mins followed by BAPTA addition by PBX dye based FLIPR assay relative to control | Homo sapiens | 4 | ALA4118190 | cell-based format | Scientific Literature | |
| 11. | ALA4123117 | B | Inhibition of BAPTA-induced Orai1-medited store operated calcium entry in human MDA-MB-231 cells at 100 uM pretreated for 15 mins followed by BAPTA addition by PBX dye based FLIPR assay relative to control | Homo sapiens | 44 | ALA4118190 | cell-based format | Scientific Literature | |
| 12. | ALA4123118 | B | Inhibition of BAPTA-induced Orai1-medited store operated calcium entry in human MDA-MB-231 cells at 10 uM pretreated for 15 mins followed by BAPTA addition by PBX dye based FLIPR assay relative to control | Homo sapiens | 3 | ALA4118190 | cell-based format | Scientific Literature | |
| 13. | ALA4123131 | B | Inhibition of BAPTA-induced Orai1-medited store operated calcium entry in human MDA-MB-231 cells at 300 uM pretreated for 15 mins followed by BAPTA addition by PBX dye based FLIPR assay relative to control | Homo sapiens | 2 | ALA4118190 | cell-based format | Scientific Literature | |
| 14. | ALA4477446 | B | Antagonist activity at human ORAI1 expressed in HEK293T cells assessed as inhibition of capsaicin-induced inwardly rectifying current at 10 uM and -120 mV by whole cell patch-clamp method | Homo sapiens | 1 | ALA4477174 | cell-based format | Scientific Literature | |
| 15. | ALA4477449 | B | Antagonist activity at human ORAI1 expressed in HEK293T cells assessed as inhibition of capsaicin-induced inwardly rectifying current at -120 mV by whole cell patch-clamp method | Homo sapiens | 1 | ALA4477174 | cell-based format | Scientific Literature | |
| 16. | ALA4477450 | B | Antagonist activity at human ORAI1 expressed in HEK293T cells assessed as inhibition of capsaicin induced inwardly rectifying current at 30 uM and -120 mV by whole cell patch-clamp method | Homo sapiens | 1 | ALA4477174 | cell-based format | Scientific Literature | |
| 17. | ALA4477451 | B | Antagonist activity at human ORAI1 expressed in HEK293T cells assessed as inhibition of capsaicin-induced inwardly rectifying current at 90 uM and -120 mV by whole cell patch-clamp method relative to control | Homo sapiens | 1 | ALA4477174 | cell-based format | Scientific Literature | |
| 18. | ALA4477452 | B | Antagonist activity at human ORAI1 expressed in HEK293T cells assessed as capsaicin-induced inwardly rectifying current at 90 uM and -120 mV by whole cell patch-clamp method relative to control | Homo sapiens | 5 | ALA4477174 | cell-based format | Scientific Literature | |
| 19. | ALA4844240 | B | Inhibition of Orai1 in human Jurkat cells assessed as CaCl2-induced increase in calcium level at 30 uM incubated for 30 mins by Fluo-4 dye based fluorescence microscopy | Homo sapiens | 3 | ALA4842361 | cell-based format | Scientific Literature |
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