# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3791613 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced p38 phosphorylation preincubated for 30 mins followed by LPS addition measured after 20 mins by Western blot analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
2. | ALA3791614 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as inhibition of LPS-induced TNF-alpha production at 10 uM incubated for 24 hrs by ELISA relative to control | Mus musculus | 4 | single protein format | Scientific Literature | ||
3. | ALA3791616 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as inhibition of LPS-induced TNF-alpha production incubated for 24 hrs by ELISA | Mus musculus | 4 | single protein format | Scientific Literature | ||
4. | ALA3791618 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as inhibition of LPS-induced IL-6 production incubated for 24 hrs by ELISA | Mus musculus | 4 | single protein format | Scientific Literature | ||
5. | ALA3791619 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced TNF-alpha mRNA expression at 10 uM preincubated for 30 mins followed by LPS addition measured after 6 hrs by qRT-PCR method | Mus musculus | 1 | assay format | Scientific Literature | ||
6. | ALA3791620 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced IL-1beta mRNA expression at 10 uM preincubated for 30 mins followed by LPS addition measured after 6 hrs by qRT-PCR method | Mus musculus | 1 | assay format | Scientific Literature | ||
7. | ALA3791621 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced IL-6 mRNA expression at 10 uM preincubated for 30 mins followed by LPS addition measured after 6 hrs by qRT-PCR method | Mus musculus | 1 | assay format | Scientific Literature | ||
8. | ALA3791622 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced COX2 mRNA expression at 10 uM preincubated for 30 mins followed by LPS addition measured after 6 hrs by qRT-PCR method | Mus musculus | 1 | assay format | Scientific Literature | ||
9. | ALA3791623 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced PGES mRNA expression at 10 uM preincubated for 30 mins followed by LPS addition measured after 6 hrs by qRT-PCR method | Mus musculus | 1 | assay format | Scientific Literature | ||
10. | ALA3791624 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced JNK phosphorylation preincubated for 30 mins followed by LPS addition measured after 20 mins by Western blot analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
11. | ALA3791625 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as decrease in LPS-induced ERK phosphorylation preincubated for 30 mins followed by LPS addition measured after 20 mins by Western blot analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
12. | ALA3791626 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as inhibition of LPS-induced NF-kappaB p65 subunit nuclear translocation at 10 uM preincubated for 30 mins followed by LPS addition measured after 1 hr by DAPI staining based fluorescence microscopic analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
13. | ALA3791627 | B | Inhibition of MD2/TLR4 complex formation in mouse RAW264.7 cells assessed as inhibition of LPS-induced NF-kappaB transcriptional activity preincubated for 30 mins followed by LPS addition measured after 6 hrs by luciferase reporter gene assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
14. | ALA3791628 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as reversal of LPS-induced IkB-alpha degradation preincubated for 30 mins followed by LPS addition measured after 1 hr by DAPI staining based fluorescence microscopic analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
15. | ALA3791629 | B | Inhibition of MD2/TLR4 complex formation in C57BL/6 mouse primary peritoneal macrophages assessed as reversal of LPS-induced IkB-alpha degradation at 10 uM preincubated for 30 mins followed by LPS addition measured after 1 hr by DAPI staining based fluorescence microscopic analysis | Mus musculus | 2 | single protein format | Scientific Literature | ||
16. | ALA3789077 | B | Inhibition of LPS-induced MD2/TLR4 complex formation in mouse macrophages at 10 uM preincubated followed by LPS addition by immunoprecipitation assay | Mus musculus | 1 | single protein format | Scientific Literature | ||
17. | ALA4133222 | B | Inhibition of FITC-labeled LPS binding to MD2 in ICR mouse primary peritoneal macrophages after 30 mins by flow cytometric analysis | Mus musculus | 3 | single protein format | Scientific Literature | ||
18. | ALA4133224 | B | Inhibition of LPS-induced MD2/TLR4 complex formation in ICR mouse primary peritoneal macrophages at 10 uM by Western blot analysis | Mus musculus | 3 | single protein format | Scientific Literature | ||
19. | ALA4143120 | B | Inhibition of FITC-LPS binding to MD2 in ICR mouse peritoneal macrophages preincubated with cells followed by FITC-LPS addition after 30 mins by flow cytometric analysis | Mus musculus | 1 | assay format | Scientific Literature | ||
20. | ALA4197378 | B | Inhibition of MD2 in C57BL/6 mouse primary peritoneal macrophages assessed as reduction in LPS-induced TLR4 activation-mediated ERK phosphorylation at 2.5 uM preincubated for 30 mins followed by LPS addition measured after 20 mins by Western blot analysis | Mus musculus | 2 | single protein format | Scientific Literature |