#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1285499	B	Inhibition of coxsackievirus B3 3C protease by fluorescence plate reader analysis	Coxsackievirus B3	9	ALA1275368	assay format	Scientific Literature
2.	ALA1285500	B	Inhibition of coxsackievirus B3 3C protease at 50 uM by fluorescence plate reader analysis	Coxsackievirus B3	12	ALA1275368	assay format	Scientific Literature
3.	ALA2166013	B	Inhibition of Coxsackievirus B3 protease 3c using NMA-EALFQGPPVK-DNP as substrate incubated for 5 mins prior to substrate addition measured after 1 hr by FRET assay	Coxsackievirus B3	14	ALA2163242	assay format	Scientific Literature
4.	ALA2166014	B	Inhibition of Coxsackievirus B3 protease 3c using NMA-EALFQGPPVK-DNP as substrate at 10 uM incubated for 5 mins prior to substrate addition measured after 1 hr by FRET assay	Coxsackievirus B3	1	ALA2163242	assay format	Scientific Literature
5.	ALA2399649	B	Inhibition of Coxsackievirus B3 Nancy 3Cpro	Coxsackievirus B3 (strain Nancy)	1	ALA2396618	single protein format	Scientific Literature
6.	ALA3239463	B	Inhibition of His-6-tagged Coxsackievirus B3 3C protease expressed in Escherichia coli BL21(DE3) using Asp-Glu-EDANS-(5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid)-MSAIFQGPISK-DABCYL (4-((4-(dimethylamino)phenyl)azo)benzoic acid) as peptide substrate at 100 uM by fluorescence-based absorbance assay	Coxsackievirus B3	13	ALA3232942	assay format	Scientific Literature
7.	ALA3239464	B	Inhibition of His-6-tagged Coxsackievirus B3 3C protease expressed in Escherichia coli BL21(DE3) using Asp-Glu-EDANS-(5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid)-MSAIFQGPISK-DABCYL (4-((4-(dimethylamino)phenyl)azo)benzoic acid) as peptide substrate by fluorescence-based absorbance assay	Coxsackievirus B3	10	ALA3232942	assay format	Scientific Literature
8.	ALA3856822	B	Inhibition of recombinant Coxsackievirus B3 3C protease expressed in Escherichia coli BL21 (DE3) preincubated for 5 mins followed by addition of NMA-EALFQGPPVK-DNP-rrr-NH2 as substrate measured up to 2 hrs by FRET-based enzyme assay	Coxsackievirus B3	59	ALA3856195	assay format	Scientific Literature
9.	ALA3856823	B	Inhibition of recombinant Coxsackievirus B3 C-terminal 6His-tagged 3C protease expressed in Escherichia coli BL21 preincubated for 20 mins followed by addition of fluorogenic peptide as substrate by Cheng-Prusoff equation analysis	Coxsackievirus B3	1	ALA3856195	assay format	Scientific Literature
10.	ALA3856824	B	Inhibition of recombinant Coxsackievirus B3 3C protease expressed in Escherichia coli BL21 (DE3) preincubated for 5 mins followed by addition of NMA-EALFQGPPVK-DNP-rrr-NH2 as substrate measured every 10 mins for 2 hrs by FRET-based enzyme assay	Coxsackievirus B3	2	ALA3856195	assay format	Scientific Literature
11.	ALA3856828	B	Non-competitive inhibition of recombinant Coxsackievirus B3 3C protease expressed in Escherichia coli BL21 (DE3) preincubated with protein followed by addition of NMA-EALFQGPPVK-DNP-rrr-NH2 as substrate measured every 5 mins for 2 hrs by Michaelis-Menten plot analysis	Coxsackievirus B3	1	ALA3856195	assay format	Scientific Literature
12.	ALA3856829	B	Competitive inhibition of recombinant Coxsackievirus B3 3C protease expressed in Escherichia coli BL21 (DE3) preincubated with protein followed by addition of NMA-EALFQGPPVK-DNP-rrr-NH2 as substrate measured every 5 mins for 2 hrs by Dixon plot analysis	Coxsackievirus B3	1	ALA3856195	assay format	Scientific Literature
13.	ALA3856830	B	Mixed-type inhibition of recombinant Coxsackievirus B3 3C protease expressed in Escherichia coli BL21 (DE3) preincubated with protein followed by addition of NMA-EALFQGPPVK-DNP-rrr-NH2 as substrate measured every 5 mins for 2 hrs by Lineweaver-Burk plot analysis	Coxsackievirus B3	1	ALA3856195	assay format	Scientific Literature
14.	ALA4028424	B	Inhibition of Coxsackievirus B3 C-terminal His6-tagged protease 3C expressed in Escherichia coli BL21(DE3) at 650 uM using N-Dabcyl-KTLEALFQGPPVYE-(Edans)-NH2 as substrate after 30 mins by fluorescence assay relative to control	Coxsackievirus B3	9	ALA4024796	assay format	Scientific Literature
15.	ALA4028426	B	Inhibition of Coxsackievirus B3 C-terminal His6-tagged protease 3C expressed in Escherichia coli BL21(DE3) at 500 uM using N-Dabcyl-KTLEALFQGPPVYE-(Edans)-NH2 as substrate preincubated up to 60 mins followed by substrate addition by fluorescence assay	Coxsackievirus B3	1	ALA4024796	assay format	Scientific Literature
16.	ALA4028430	B	Inhibition of Coxsackievirus B3 C-terminal His6-tagged protease 3C expressed in Escherichia coli BL21(DE3) at 100 uM using N-Dabcyl-KTLEALFQGPPVYE-(Edans)-NH2 as substrate preincubated up to 60 mins followed by substrate addition by fluorescence assay	Coxsackievirus B3	1	ALA4024796	assay format	Scientific Literature
17.	ALA4028431	B	Inhibition of Coxsackievirus B3 C-terminal His6-tagged protease 3C expressed in Escherichia coli BL21(DE3) using N-Dabcyl-KTLEALFQGPPVYE-(Edans)-NH2 as substrate after 30 mins by fluorescence assay	Coxsackievirus B3	2	ALA4024796	assay format	Scientific Literature
18.	ALA4028432	B	Irreversible inhibition of Coxsackievirus B3 C-terminal His6-tagged protease 3C expressed in Escherichia coli BL21(DE3) assessed as ratio of Kinact to Ki using N-Dabcyl-KTLEALFQGPPVYE-(Edans)-NH2 as substrate after 30 mins by fluorescence assay	Coxsackievirus B3	3	ALA4024796	assay format	Scientific Literature
19.	ALA4028434	B	Irreversible inhibition of Coxsackievirus B3 C-terminal His6-tagged protease 3C expressed in Escherichia coli BL21(DE3) after 60 mins by LC-MS analysis	Coxsackievirus B3	1	ALA4024796	assay format	Scientific Literature
20.	ALA4028435	B	Irreversible inhibition of Coxsackievirus B3 C-terminal His6-tagged protease 3C expressed in Escherichia coli BL21(DE3) assessed as protein-compound adduct formation after 60 mins by LC-MS analysis	Coxsackievirus B3	1	ALA4024796	assay format	Scientific Literature