# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2404522 | B | Activity at mouse SPHK1 transfected in mouse A20/2J cell lysates assessed as compound phosphorylation at 10 uM after 30 mins by autoradiography in presence of [gamma-32P]ATP | Mus musculus | 2 | cell-based format | Scientific Literature | ||
2. | ALA2404703 | B | Inhibition of mouse SPHK1 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 2 to 4 uM incubated for 5 mins prior to substrate addition measured after 5 mins | Mus musculus | 2 | cell-based format | Scientific Literature | ||
3. | ALA2404705 | B | Inhibition of mouse SPHK1 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 8 uM incubated for 5 mins prior to substrate addition measured after 5 mins | Mus musculus | 1 | cell-based format | Scientific Literature | ||
4. | ALA2412213 | B | Inhibition of mouse SphK1 | Mus musculus | 2 | single protein format | Scientific Literature | ||
5. | ALA3816951 | B | Inhibition of SphK1 in mouse erythrocytes using sphingosine as substrate by luminescence assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
6. | ALA4051841 | B | Inhibition of recombinant mouse SPHK1 using sphingosine as substrate in presence ATP | Mus musculus | 1 | single protein format | Scientific Literature | ||
7. | ALA4357631 | B | Inhibition of recombinant mouse SphK1 expressed in baculovirus infected Sf9 insect cells using D-erythro-sphingosine as substrate measured after 30 mins in presence of [gamma32P]-ATP by liquid scintillation counting method | Mus musculus | 1 | cell-based format | Scientific Literature | ||
8. | ALA5167618 | B | Inhibition of recombinant mouse SphK1 transfected in HEK293T cells using D-erythro-sphingosine measured after 20 mins in presence of gamma-[p32]-ATP by autoradiography and liquid scintillation counting method | Mus musculus | 1 | cell-based format | Scientific Literature | ||
9. | ALA5226994 | B | Inhibition of recombinant mouse SphK1 expressed in baculovirus infected Sf9 cells using D-erythro-sphingosine and [gamma-32P]ATP as substrate incubated for 30 mins by liquid scintillation counting analysis | Mus musculus | 2 | cell-based format | Scientific Literature |