Inhibition of bovine kidney intestinal alkaline phosphatase using p-NPP as substrate treated 10 mins before substrate addition measured after 30 mins by spectrophotometric assay
Inhibition of bovine kidney non-specific alkaline phosphatase using CDP-star chemiluminescent substrate assessed as change in luminescence by spectrophotometric method
Inhibition of bovine kidney non-specific alkaline phosphatase using CDP-star chemiluminescent substrate assessed as change in luminescence at 0.2 mM by spectrophotometric method
Prodrug conversion in glycine buffer assessed as bovine intestinal mucosal alkaline phosphatase-mediated triptolide and formaldehyde formation at pH 9.8
Drug level in glycine buffer treated with Sodium (((5bS,6aS,7aR,8R,8aS,9aS,9bS,10aS,10bS)-8a-Isopropyl-10b-methyl-3-oxo-1,2,3,5,5b,6,6a,8,8a,9a,9b,10b-dodecahydrotris-(oxireno)[2',3':4b,5;'',3'':6,7;2''',3''':8a,9]phenanthro[1,2-c]furan-8-yl)oxy)methyl Phosphate at pH 9.8 in presence of bovine intestinal mucosal alkaline phosphatase
Competitive inhibition of bovine tissue non-specific alkaline phosphatase at 0.5 to 2 uM preincubated for 3 to 5 mins followed by CDP-star substrate addition measured after 15 mins by double-reciprocal plot analysis
Inhibition of bovine tissue non-specific alkaline phosphatase at 0.2 mM preincubated for 3 to 5 mins followed by CDP-star substrate addition measured after 15 mins by luminescence assay
Inhibition of bovine tissue non-specific alkaline phosphatase preincubated for 3 to 5 mins followed by CDP-star substrate addition measured after 15 mins by luminescence assay
Inhibition of bovine TNAP using CDP-star as substrate preincubated for 5 to 7 mins followed by substrate addition measured after 15 mins by spectrophotometric method
Inhibition of bovine TNAP at 0.2 mM using CDP-star as substrate preincubated for 5 to 7 mins followed by substrate addition measured after 15 mins by spectrophotometric method