# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2411907 | B | Inhibition of USP9x in human Z138 cells after 1 hr by immunoblotting analysis | Homo sapiens | 1 | ALA2406999 | cell-based format | Scientific Literature | |
2. | ALA3118415 | B | Inhibition of Usp9x in human Z138 cells using HA-Ub vinyl-sulfone as substrate at 1.25 to 5 uM after 4 hrs by immunoblotting analysis | Homo sapiens | 2 | ALA3112519 | cell-based format | Scientific Literature | |
3. | ALA3118416 | B | Inhibition of human recombinant N-terminal His6-Smt3-fusion-tagged Usp9x catalytic domain expressed in Escherichia coli using Ub-AMC as substrate incubated for 30 mins prior to substrate addition by fluorescence assay | Homo sapiens | 2 | ALA3112519 | assay format | Scientific Literature | |
4. | ALA4257308 | B | Inhibition of N-terminal His6-Smt3-fusion tagged human Usp9x catalytic domain (1553 to 1960 residues) expressed in Escherichia coli pre- incubated for 30 mins before Ub-AMC substrate addition by fluorescence based assay | Homo sapiens | 10 | ALA4257227 | assay format | Patent Bioactivity Data | |
5. | ALA4257309 | B | Inhibition of Usp9x in human Z138 cells at 1.25 to 5 uM incubated for 4 hrs by SDS-PAGE and immunoblotting | Homo sapiens | 3 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
6. | ALA4257310 | B | Inhibition of Usp9x in human Z138 cells assessed as reduction in Mcl-1 protein level at 1.25 to 5 uM incubated for 4 hrs by SDS-PAGE and immunoblotting | Homo sapiens | 3 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
7. | ALA4257312 | B | Inhibition of Usp9x in human MM1.S cells at 5 uM incubated for 5 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
8. | ALA4257313 | B | Inhibition of Usp9x in human MM1.S cells at 5 uM incubated for 60 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
9. | ALA4257314 | B | Inhibition of Usp9x in primary tumor cells from patient with plasma cell leukemia at 5 uM incubated for 5 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
10. | ALA4257315 | B | Inhibition of Usp9x in primary tumor cells from patient with plasma cell leukemia at 5 uM incubated for 60 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
11. | ALA4257316 | B | Inhibition of Usp9x in human MM1.S cells assessed as reduction in MCL-1 protein levels at 5 uM incubated for 5 to 240 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
12. | ALA4257317 | B | Inhibition of Usp9x in human MM1.S cells assessed as reduction in caspase activity by measuring cleaved PARP protein levels at 5 uM incubated for 5 to 240 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
13. | ALA4257318 | B | Inhibition of Usp9x in in primary tumor cells from patient with plasma cell leukemia assessed as reduction in MCL-1 protein levels at 5 uM incubated for 30 to 240 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
14. | ALA4257340 | B | Inhibition of Usp9x in human A375 cells | Homo sapiens | 1 | ALA4257227 | cell-based format | Patent Bioactivity Data | |
15. | ALA4257393 | B | Inhibition of USP9x in Vemurafenib sensitive human A375 cells | Homo sapiens | 1 | ALA4257227 | assay format | Patent Bioactivity Data | |
16. | ALA4257394 | B | Inhibition of USP9x in Vemurafenib resistant human A375 cells | Homo sapiens | 1 | ALA4257227 | assay format | Patent Bioactivity Data | |
17. | ALA4257397 | B | Inhibition of USP9x in Vemurafenib sensitive human A375 cells assessed as STAT3 phosphorylation | Homo sapiens | 1 | ALA4257227 | assay format | Patent Bioactivity Data | |
18. | ALA4257398 | B | Inhibition of USP9x in Vemurafenib resistant human A375 cells assessed as STAT3 phosphorylation | Homo sapiens | 1 | ALA4257227 | assay format | Patent Bioactivity Data | |
19. | ALA4372582 | B | Inhibition of USP9x in HEK293T cell lysates assessed as enzyme recovery at 250 uM using HA-tagged vinyl-sulfone as substrate preincubated for 40 mins followed by 10-fold dilution and subsequent substrate addition measured up to 2 hrs by Western blot analysis | Homo sapiens | 1 | ALA4371052 | subcellular format | Scientific Literature | |
20. | ALA4372592 | B | Inhibition of USP9x in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | ALA4371052 | subcellular format | Scientific Literature |