# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5251112 | B | Inhibition of human USP9X (1551 to 1970 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
2. | ALA5251141 | B | Inhibition of human USP9X using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
3. | ALA5251152 | B | Inhibition of USP9X (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
4. | ALA2411907 | B | Inhibition of USP9x in human Z138 cells after 1 hr by immunoblotting analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
5. | ALA3118415 | B | Inhibition of Usp9x in human Z138 cells using HA-Ub vinyl-sulfone as substrate at 1.25 to 5 uM after 4 hrs by immunoblotting analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
6. | ALA3118416 | B | Inhibition of human recombinant N-terminal His6-Smt3-fusion-tagged Usp9x catalytic domain expressed in Escherichia coli using Ub-AMC as substrate incubated for 30 mins prior to substrate addition by fluorescence assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
7. | ALA4257308 | B | Inhibition of N-terminal His6-Smt3-fusion tagged human Usp9x catalytic domain (1553 to 1960 residues) expressed in Escherichia coli pre- incubated for 30 mins before Ub-AMC substrate addition by fluorescence based assay | Homo sapiens | 10 | assay format | Patent Bioactivity Data | ||
8. | ALA4257309 | B | Inhibition of Usp9x in human Z138 cells at 1.25 to 5 uM incubated for 4 hrs by SDS-PAGE and immunoblotting | Homo sapiens | 3 | cell-based format | Patent Bioactivity Data | ||
9. | ALA4257310 | B | Inhibition of Usp9x in human Z138 cells assessed as reduction in Mcl-1 protein level at 1.25 to 5 uM incubated for 4 hrs by SDS-PAGE and immunoblotting | Homo sapiens | 3 | cell-based format | Patent Bioactivity Data | ||
10. | ALA4257312 | B | Inhibition of Usp9x in human MM1.S cells at 5 uM incubated for 5 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
11. | ALA4257313 | B | Inhibition of Usp9x in human MM1.S cells at 5 uM incubated for 60 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
12. | ALA4257314 | B | Inhibition of Usp9x in primary tumor cells from patient with plasma cell leukemia at 5 uM incubated for 5 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
13. | ALA4257315 | B | Inhibition of Usp9x in primary tumor cells from patient with plasma cell leukemia at 5 uM incubated for 60 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
14. | ALA4257316 | B | Inhibition of Usp9x in human MM1.S cells assessed as reduction in MCL-1 protein levels at 5 uM incubated for 5 to 240 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
15. | ALA4257317 | B | Inhibition of Usp9x in human MM1.S cells assessed as reduction in caspase activity by measuring cleaved PARP protein levels at 5 uM incubated for 5 to 240 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
16. | ALA4257318 | B | Inhibition of Usp9x in in primary tumor cells from patient with plasma cell leukemia assessed as reduction in MCL-1 protein levels at 5 uM incubated for 30 to 240 mins by by SDS-PAGE and immunoblotting | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
17. | ALA4257340 | B | Inhibition of Usp9x in human A375 cells | Homo sapiens | 1 | cell-based format | Patent Bioactivity Data | ||
18. | ALA4257393 | B | Inhibition of USP9x in Vemurafenib sensitive human A375 cells | Homo sapiens | 1 | assay format | Patent Bioactivity Data | ||
19. | ALA4257394 | B | Inhibition of USP9x in Vemurafenib resistant human A375 cells | Homo sapiens | 1 | assay format | Patent Bioactivity Data | ||
20. | ALA4257397 | B | Inhibition of USP9x in Vemurafenib sensitive human A375 cells assessed as STAT3 phosphorylation | Homo sapiens | 1 | assay format | Patent Bioactivity Data |