# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2422850 | Binding | Inhibition of Bacillus subtilis SU570 GFP-fused FtsZ assessed as perturbation of Z-ring formation at 12 uM after 60 mins by fluorescence microscopic analysis | Bacillus subtilis | 1 | ALA2417459 | single protein format | Scientific Literature | |
2. | ALA2422851 | Binding | Inhibition of Bacillus subtilis FtsZ GTPase activity assessed as production of inorganic phosphate measured up to 60 mins by malachite green-phosphomolybdate colorimetric assay | Bacillus subtilis | 1 | ALA2417459 | single protein format | Scientific Literature | |
3. | ALA2423036 | Binding | Displacement of [3H]-GTP from Bacillus subtilis FtsZ assessed as equilibrium association constant by scintillation counting assay | Bacillus subtilis | 1 | ALA2417459 | single protein format | Scientific Literature | |
4. | ALA2423037 | Binding | Displacement of mant-GTP from Bacillus subtilis FtsZ by fluorescence anisotropy analysis | Bacillus subtilis | 1 | ALA2417459 | single protein format | Scientific Literature | |
5. | ALA2423038 | Binding | Displacement of mant-GTP from Bacillus subtilis FtsZ assessed as equilibrium association constant by fluorescence anisotropy analysis | Bacillus subtilis | 1 | ALA2417459 | single protein format | Scientific Literature | |
6. | ALA4121904 | Binding | Inhibition of FtsZ in penicillin-susceptible Bacillus subtilis ATCC 9372 assessed as increase in length of bacteria at 8 ug/ml after 5 hrs by phase-contrast light microscopic method | Bacillus subtilis | 1 | ALA4118132 | single protein format | Scientific Literature | |
7. | ALA4121906 | Binding | Inhibition of FtsZ in penicillin-susceptible Bacillus subtilis ATCC 9372 assessed as increase in length of bacteria at 0.125 ug/ml after 5 hrs by phase-contrast light microscopic method | Bacillus subtilis | 1 | ALA4118132 | single protein format | Scientific Literature | |
8. | ALA4121908 | Binding | Inhibition of penicillin-susceptible Bacillus subtilis ATCC 9372 N-terminal His6-tagged FtsZ expressed in Escherichia coli BL21 (DE3) cells by fluorescence spectrophotometer-based light scattering assay | Bacillus subtilis | 1 | ALA4118132 | cell-based format | Scientific Literature | |
9. | ALA4121909 | Binding | Inhibition of penicillin-susceptible Bacillus subtilis ATCC 9372 N-terminal His6-tagged FtsZ expressed in Escherichia coli BL21 (DE3) cells at 10 ug/ml by fluorescence spectrophotometer-based light scattering assay | Bacillus subtilis | 1 | ALA4118132 | cell-based format | Scientific Literature | |
10. | ALA4188576 | Binding | Inhibition of FtsZ in Bacillus subtilis ATCC 9372 assessed as filamentation at 1 ug/ml | Bacillus subtilis | 1 | ALA4184277 | single protein format | Scientific Literature | |
11. | ALA4188577 | Binding | Inhibition of FtsZ in Bacillus subtilis ATCC 9372 assessed as filamentation | Bacillus subtilis | 5 | ALA4184277 | single protein format | Scientific Literature | |
12. | ALA4428180 | Binding | Inhibition of Bacillus subtilis FtsZ assessed as reduction in GTPase activity by malachite green assay | Bacillus subtilis | 1 | ALA4428024 | single protein format | Scientific Literature | |
13. | ALA4838263 | Binding | Induction of Bacillus subtilis FtsZ polymerization by fluorescence spectrophotometry | Bacillus subtilis | 1 | ALA4837210 | single protein format | Scientific Literature | |
14. | ALA4838264 | Binding | Induction of Bacillus subtilis FtsZ polymerization assessed as formation of bundling of FtsZ protofilaments at 10 ug/ml after 20 mins by transmission electron microscopic analysis | Bacillus subtilis | 1 | ALA4837210 | single protein format | Scientific Literature | |
15. | ALA4838271 | Binding | Inhibition of FtsZ in Bacillus subtilis assessed as disruption of Z ring formation | Bacillus subtilis | 1 | ALA4837210 | single protein format | Scientific Literature | |
16. | ALA5034076 | Binding | Binding affinity to Bacillus subtilis FtsZ polymer assessed as dissociation constant by fluorescence anisotropy analysis | Bacillus subtilis | 18 | ALA5033991 | single protein format | Scientific Literature | |
17. | ALA5034086 | Binding | Inhibition of GFP-tagged FtsZ in Bacillus subtilis SU570 assessed as induction of irregular shaped z-ring formation at 0.2 uM incubated for 1.5 hrs | Bacillus subtilis | 1 | ALA5033991 | single protein format | Scientific Literature | |
18. | ALA5034087 | Binding | Inhibition of GFP-tagged FtsZ in Bacillus subtilis SU570 assessed as delocalization into punctate foci at 0.2 uM incubated for 1.5 hrs | Bacillus subtilis | 1 | ALA5033991 | single protein format | Scientific Literature | |
19. | ALA5034088 | Binding | Competitive binding affinity to Bacillus subtilis FtsZ at 10 uM in presence of PC190723 by fluorescence anisotropy analysis | Bacillus subtilis | 5 | ALA5033991 | single protein format | Scientific Literature | |
20. | ALA5034089 | Binding | Induction of Bacillus subtilis FtsZ polymerization at 0.1 mM measured after 5 to 10 mins by fluorescence anisotropy analysis | Bacillus subtilis | 1 | ALA5033991 | single protein format | Scientific Literature |