| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2429073 | B | Inhibition of UHRF1 (unknown origin) after 30 mins by AlphaScreen assay | Homo sapiens | 56 | single protein format | Scientific Literature | ||
| 2. | ALA3136679 | B | Inhibition of UHRF1 tandem tudor domain (121 to 286) (unknown origin) expressed in Escherichia coli BL21 Rosetta DE3 using H3K9Me3 as substrate incubated for 30 mins at room temperature followed by incubation under dark condition for 30 mins by AlphaScreen assay | Homo sapiens | 25 | assay format | Scientific Literature | ||
| 3. | ALA3214895 | B | PubChem BioAssay. Counterscreen for inhibitors of 5-meCpG-binding domain protein 2 (MBD2): TRFRET-based biochemical high throughput dose response assay to identify inhibitors of binding of ubiquitin-like with PHD and ring finger domains 1 (UHRF1) to methylated oligonucleotide. (Class of assay: confirmatory) | 46 | single protein format | PubChem BioAssays | |||
| 4. | ALA3784457 | B | Inhibition of UHRF1 in human MCF7 cells assessed as decrease in methylation at RAR beta exon at 15 uM after 72 hrs by methylation specific-PCR method | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 5. | ALA3784458 | B | Inhibition of UHRF1 in human MCF7 cells assessed as DNA global demethylation by measuring 5-methylcytosine level at 15 uM after 72 hrs by HPLC-MS/MS analysis (Rvb = 1.7 +/- 0.36%) | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 6. | ALA3784459 | B | Inhibition of UHRF1/DNMT1 protein interaction in human U251 cells at 15 uM after 4 hrs by DAPI staining based P-LISA | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA3784460 | B | Inhibition of UHRF1/DNMT1 protein interaction in human U251 cells assessed as reduction in 5-methylcytosine level at 15 uM after 72 hrs by ELISA relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3784461 | B | Inhibition of UHRF1 in human MCF7 cells assessed as reduction in global 5-methylcytosine level at 15 uM after 72 hrs by HPLC-MS/MS analysis relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 9. | ALA3859080 | B | Binding affinity to UHRF1 (unknown origin) at 200 uM by DSF assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 10. | ALA4194971 | B | Binding affinity to 6His-tagged and human UHRF1 tandem TUDOR domain (126 to 285 residues) expressed in Escherichia coli BL21(DE3)-T1R by ITC method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA4377337 | B | Binding affinity to UHRF1 (unknown origin) assessed as induction of thermal shifts at 200 uM measured for 25 mins by SYPRO orange dye thermal shift assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 12. | ALA4842138 | B | Binding affinity to UHRF1 TTD (unknown origin) assessed as increase in thermal stability by measuring change in melting temperature by SYPRO orange dye based fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA4842139 | B | Binding affinity to UHRF1 TTD (unknown origin) assessed as dissociation constant by ITC analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA4842140 | B | Binding affinity to UHRF1 TTD (unknown origin) assessed as change in enthalpy by ITC analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4842141 | B | Binding affinity to UHRF1 Y188A/Y191A double mutant (unknown origin) assessed as dissociation constant by ITC analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA4842142 | B | Binding affinity to UHRF1 TTD variant loop (unknown origin) assessed as dissociation constant by ITC analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA4842143 | B | Displacement of FAM-labeled H3K9me3 from UHRF1 TTD(unknown origin) by fluorescence polarization assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA4842144 | B | Displacement of FAM-labeled LIG1K126me3 from UHRF1 TTD(unknown origin) by fluorescence polarization assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 19. | ALA4842146 | B | Binding affinity to UHRF1 TTD (unknown origin) assessed as compound-protein complex stoichiometry ratio of 1:1 by ITC analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 20. | ALA4842147 | B | Binding affinity to UHRF1 M224A mutant (unknown origin) assessed as dissociation constant by ITC analysis | Homo sapiens | 1 | single protein format | Scientific Literature |
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