| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2409989 | B | Activation of human recombinant AMPK alpha1beta2gamma1 | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
| 2. | ALA3807381 | B | Binding affinity to human recombinant N-terminal AVI-tagged phosphorylated AMPK alpha1beta2gamma1 expressed in Escherichia coli BL21 cells after 1 to 10 mins by biolayer interferometry | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA3854702 | B | Activation of recombinant human AMPK alpha1/beta2/gamma1 using Cy5-labelled SAMS as substrate in presence of ATP by TR-FRET assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 4. | ALA3854797 | B | Activation of recombinant human AMPK alpha1/beta2/gamma1 assessed as protection from Thr172 residue dephosphorylation up to 40 uM preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 5. | ALA3859873 | B | Activation of human recombinant AMPK alpha1/beta2/gamma1 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3859874 | B | Activation of human recombinant AMPK alpha1/beta2/gamma1 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4023988 | B | Allosteric activation of human AMPK alpha1/beta2/gamma1 by TR-FRET assay | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
| 8. | ALA4026637 | B | Agonist activity at human recombinant phosphorylated AMPK complex 4 alpha1/beta2/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4026648 | B | Agonist activity at human recombinant phosphorylated AMPK complex 4 alpha1/beta2/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate at 2 nM to 50 uM preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4134773 | B | Activation of human recombinant AMPKalpha1beta2gamma1 expressed in Escherichia coli at 20 uM incubated for 1 hr using serine/threonine kinase substrate | Homo sapiens | 7 | assay format | Scientific Literature | ||
| 11. | ALA4134781 | B | Activation of human recombinant AMPKalpha1beta2gamma1 expressed in Escherichia coli at 50 nM incubated for 1 hr using serine/threonine kinase substrate | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA4134789 | B | Activation of human recombinant AMPKalpha1beta2gamma1 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA4144384 | B | Activation of CaMKK2-phosphorylated AMPK4 alpha1/beta2/gamma1 (unknown origin) using fluorescein-labeled SAMS peptide as substrate pretreated for 30 mins in presence of AMP followed by substrate addition measured after 60 mins by fluorescence assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 14. | ALA4178059 | B | Inhibition of human AMPKalpha1 (11 to 559 residues)/beta2 (1 to 272 residues)/gamma1 (1 to 331 residues) assessed as residual activity at 500 nM in presence of 33P-ATP by filter binding assay relative to control | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 15. | ALA4234638 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha1beta2gamma1 expressed in Escherichia coli BL21 cells at 0.5 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 16. | ALA4234642 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha1beta2gamma1 expressed in Escherichia coli BL21 cells using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 17. | ALA4887233 | B | AMPK (A1/B2/G1) Invitrogen Lab (UK) kinase screen (BI) | Homo sapiens | 1 | protein complex format | Donated Chemical Probes - SGC Frankfurt |
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