# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1012659 | B | Inhibition of beta tubulin nucleotide site assessed as inhibition of displacement of cold GDP by [alpha32P]GTP at 20 uM by rapid filtration technique | 3 | ALA1139351 | single protein format | Scientific Literature | ||
2. | ALA1012660 | B | Inhibition of beta tubulin nucleotide site assessed as inhibition of displacement of cold GDP by [alpha32P]GTP at 40 uM by rapid filtration technique | 1 | ALA1139351 | single protein format | Scientific Literature | ||
3. | ALA1012661 | B | Inhibition of beta tubulin nucleotide site assessed as displacement of cold GDP by [alpha32P]GTP at 100 uM by rapid filtration technique | 1 | ALA1139351 | single protein format | Scientific Literature | ||
4. | ALA1012662 | B | Inhibition of beta tubulin nucleotide site assessed as inhibition of displacement of cold GDP by [alpha32P]GTP by rapid filtration technique relative to phomopsin A | 1 | ALA1139351 | single protein format | Scientific Literature | ||
5. | ALA3803736 | B | Binding affinity to TUBB in HEK293 proteomes assessed as spectral count ratio at 20 uM incubated for 16 hrs by TAMRA biotin-azide dye based LC-MS/MS analysis relative to control | Homo sapiens | 1 | ALA3797061 | single protein format | Scientific Literature | |
6. | ALA3993286 | B | Competitive binding affinity to beta-tubulin in human K562 cells assessed as disappearance of EBI-protein adducts at 20 to 40 uM preincubated for 2 hrs followed by EBI addition measured after 1.5 hrs by Western blot analysis | Homo sapiens | 1 | ALA3992517 | cell-based format | Scientific Literature | |
7. | ALA3993287 | B | Competitive binding affinity to beta-tubulin in human K562 cells assessed as disappearance of EBI-protein adducts at 7 to 14 uM preincubated for 2 hrs followed by EBI addition measured after 1.5 hrs by Western blot analysis | Homo sapiens | 1 | ALA3992517 | cell-based format | Scientific Literature | |
8. | ALA3993306 | B | Competitive binding affinity to beta-tubulin in human K562 cells assessed as disappearance of EBI-protein adducts at 5 uM preincubated for 2 hrs followed by EBI addition measured after 1.5 hrs by Western blot analysis | Homo sapiens | 1 | ALA3992517 | cell-based format | Scientific Literature | |
9. | ALA4002728 | B | Inhibition of tubulin beta polymerization in human MCF7 cells after 18 to 24 hrs by spectrophotometric method relative to control | Homo sapiens | 7 | ALA4002544 | cell-based format | Scientific Literature | |
10. | ALA4002731 | B | Inhibition of tubulin beta polymerization in human HepG2 cells after 18 to 24 hrs by spectrophotometric method relative to control | Homo sapiens | 10 | ALA4002544 | cell-based format | Scientific Literature | |
11. | ALA4123910 | B | Inhibition of tubulin beta polymerization in human MCF7 cells after 18 to 24 hrs by ELISA based spectrophotometric analysis | Homo sapiens | 4 | ALA4118208 | cell-based format | Scientific Literature | |
12. | ALA4123911 | B | Potency index, ratio of colchicine IC50 to compound IC50 for tubulin beta polymerization in human MCF7 cells | Homo sapiens | 1 | ALA4118208 | cell-based format | Scientific Literature | |
13. | ALA4259780 | B | Inhibition of tubulin beta polymerization in human Caco2 cells after 48 hrs by ELISA | Homo sapiens | 2 | ALA4257538 | cell-based format | Scientific Literature | |
14. | ALA4259781 | B | Inhibition of tubulin beta polymerization in human MCF7 cells after 48 hrs by ELISA | Homo sapiens | 2 | ALA4257538 | cell-based format | Scientific Literature | |
15. | ALA4270854 | B | Binding affinity to tubulin beta chain in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis | Homo sapiens | 1 | ALA4270485 | cell-based format | Scientific Literature |