| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2390479 | B | Activation of recombinant AMPK alpha2beta1gamma1 (unknown origin) at 20 ug/mL after 2 hrs by scintillation proximity assay relative to AMP | Homo sapiens | 15 | protein complex format | Scientific Literature | ||
| 2. | ALA2390480 | B | Activation of recombinant AMPK alpha2beta1gamma1 (unknown origin) after 2 hrs by scintillation proximity assay | Homo sapiens | 15 | protein complex format | Scientific Literature | ||
| 3. | ALA2406105 | B | Inhibition of AMPK A2/B1/G1 (unknown origin) at 10 uM relative to control | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 4. | ALA2409990 | B | Activation of human recombinant AMPK alpha2beta1gamma1 | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 5. | ALA3854701 | B | Activation of recombinant human AMPK alpha2/beta1/gamma1 assessed as protection from Thr172 residue dephosphorylation up to 40 uM preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 6. | ALA3854725 | B | Activation of recombinant human AMPK alpha2/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 7. | ALA3854726 | B | Activation of recombinant human AMPK alpha2/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay relative to AMP | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 8. | ALA3859863 | B | Activation of full length human recombinant AMPK alpha2/beta1/gamma1 expressed in baculovirus infected sf21 cells using 5'-FAM-SAMS peptide substrate after 45 mins by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA3859864 | B | Activation of full length human recombinant AMPK alpha2/beta1/gamma1 expressed in baculovirus infected sf21 cells using 5'-FAM-SAMS peptide substrate after 45 mins by fluorescence assay relative to AMP | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA3859871 | B | Activation of human recombinant AMPK alpha2/beta1/gamma1 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA3859872 | B | Activation of human recombinant AMPK alpha2/beta1/gamma1 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA3876046 | B | Inhibition of human recombinant full length N-terminal GST/N-terminal His-tagged AMPK alpha2/beta1/gamma1 expressed in baculovirus expression system | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA3888127 | B | In Vitro AMPK Activation Assay: The in vitro AMPK activation assay is performed in a volume of 30 μl in a 384-well plate. Enzyme reactions were assembled in the microtiter plate by adding 15 μl of 2× enzyme in assay buffer (20 mM HEPES, pH 7.3, 5 mM MgCl2, 3 mM DTT, 0.01% Brij 35 and CamK Kinase, to activate AMPK) to wells which contained either DMSO or compound. The reaction was initiated with the addition of 15 μl 2× substrate mixture containing 200 μM ATP, and 3.0 μM fluorescently labeled SAMS (5-FAM-HMRSAMSGLHLVKRR-COOH) in assay buffer. After 45-minute incubation at 25° C., the reaction was stopped by the addition of 70 μl stop buffer (100 mM HEPES, pH 7.3, 40 mM EDTA, 0.015% Brij 35). Phosphorylated 5-FAM SAMS product is assessed using a Caliper EZ Reader LabChip microfluidics reader. Product conversion is determined by calculating the peak heights of the substrate and product and reporting the product/(product+substrate) peak ratio. | Homo sapiens | 25 | protein complex format | BindingDB Database | ||
| 14. | ALA4007928 | B | Inhibition of human AMPK alpha2/beta1/gamma1 at 0.1 uM relative to control | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 15. | ALA4023987 | B | Allosteric activation of human AMPK alpha2/beta1/gamma1 by TR-FRET assay | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
| 16. | ALA4026617 | B | Agonist activity at human recombinant phosphorylated AMPK complex 7 alpha2/beta1/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate at 50 uM preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins relative to AMP | Homo sapiens | 18 | cell-based format | Scientific Literature | ||
| 17. | ALA4026619 | B | Agonist activity at human recombinant phosphorylated AMPK complex 7 alpha2/beta1/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 30 | cell-based format | Scientific Literature | ||
| 18. | ALA4026620 | B | Agonist activity at human recombinant phosphorylated AMPK complex 7 alpha2/beta1/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate at 0.002 to 50 uM preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins relative to AMP | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 19. | ALA4026633 | B | Agonist activity at human recombinant phosphorylated AMPK complex 7 alpha2/beta1/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate at 0.0008 to 16 uM preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins relative to AMP | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 20. | ALA4057264 | B | Inhibition of AMPK-alpha2/beta1/gamma1 (unknown origin) at 0.45 uM relative to control | Homo sapiens | 1 | protein complex format | Scientific Literature |
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