| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2409988 | B | Activation of human recombinant AMPK alpha2beta2gamma1 | Homo sapiens | 4 | ALA2406937 | protein complex format | Scientific Literature | |
| 2. | ALA3854703 | B | Activation of recombinant human AMPK alpha2/beta2/gamma1 using Cy5-labelled SAMS as substrate in presence of ATP by TR-FRET assay | Homo sapiens | 1 | ALA3853317 | protein complex format | Scientific Literature | |
| 3. | ALA3854798 | B | Activation of recombinant human AMPK alpha2/beta2/gamma1 assessed as protection from Thr172 residue dephosphorylation up to 40 uM preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay | Homo sapiens | 1 | ALA3853317 | protein complex format | Scientific Literature | |
| 4. | ALA3859854 | B | Activation of full length human His6-tagged AMPK alpha2/beta2/gamma1 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL using FL-Peptide 7 substrate incubated for 2 hrs by fluorescence assay | Homo sapiens | 1 | ALA3856285 | assay format | Scientific Literature | |
| 5. | ALA3859859 | B | Activation of full length human His6-tagged AMPK alpha2/beta2/gamma1 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL preincubated for 10 mins followed by FL-Peptide 7 substrate addition measured after 2 hrs by fluorescence assay | Homo sapiens | 2 | ALA3856285 | assay format | Scientific Literature | |
| 6. | ALA3859865 | B | Activation of full length human His6-tagged AMPK alpha2/beta2/gamma1 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL using FL-Peptide 7 substrate incubated for 2 hrs by fluorescence assay relative to AMP | Homo sapiens | 1 | ALA3856285 | assay format | Scientific Literature | |
| 7. | ALA3859867 | B | Activation of full length human His6-tagged AMPK alpha2/beta2/gamma1 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL preincubated for 10 mins followed by FL-Peptide 7 substrate addition measured after 2 hrs by fluorescence assay relative to AMP | Homo sapiens | 2 | ALA3856285 | assay format | Scientific Literature | |
| 8. | ALA3859875 | B | Activation of human recombinant AMPK alpha2/beta2/gamma1 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay | Homo sapiens | 1 | ALA3856285 | cell-based format | Scientific Literature | |
| 9. | ALA3859876 | B | Activation of human recombinant AMPK alpha2/beta2/gamma1 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay relative to control | Homo sapiens | 1 | ALA3856285 | cell-based format | Scientific Literature | |
| 10. | ALA4023989 | B | Allosteric activation of human AMPK alpha2/beta2/gamma1 by TR-FRET assay | Homo sapiens | 2 | ALA4020835 | protein complex format | Scientific Literature | |
| 11. | ALA4026642 | B | Agonist activity at human recombinant phosphorylated AMPK complex 10 alpha2/beta2/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | ALA4024751 | cell-based format | Scientific Literature | |
| 12. | ALA4026653 | B | Agonist activity at human recombinant phosphorylated AMPK complex 10 alpha2/beta2/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate at 2 nM to 50 uM preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | ALA4024751 | cell-based format | Scientific Literature | |
| 13. | ALA4134777 | B | Activation of human recombinant AMPKalpha2beta2gamma1 expressed in Escherichia coli at 20 uM incubated for 1 hr using serine/threonine kinase substrate | Homo sapiens | 7 | ALA4130473 | assay format | Scientific Literature | |
| 14. | ALA4134785 | B | Activation of human recombinant AMPKalpha2beta2gamma1 expressed in Escherichia coli at 50 nM incubated for 1 hr using serine/threonine kinase substrate | Homo sapiens | 1 | ALA4130473 | assay format | Scientific Literature | |
| 15. | ALA4134793 | B | Activation of human recombinant AMPKalpha2beta2gamma1 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate | Homo sapiens | 1 | ALA4130473 | assay format | Scientific Literature | |
| 16. | ALA4144370 | B | Activation of CaMKK2-phosphorylated AMPK10 alpha2/beta2/gamma1 (unknown origin) assessed as ratio of 5-(6-chloro-5-(1-methyl-1H-indol-5-yl)-1H-benzo[d]imidazol-2-yloxy)-2-methylbenzoic acid EC50 to test compound EC50 using fluorescein-labeled SAMS peptide as substrate pretreated for 30 mins in presence of AMP followed by substrate addition measured after 60 mins by fluorescence assay | Homo sapiens | 6 | ALA4138270 | protein complex format | Scientific Literature | |
| 17. | ALA4144375 | B | Activation of CaMKK2-phosphorylated AMPK10 alpha2/beta2/gamma1 (unknown origin) assessed as ratio of 5-(6-chloro-5-(1-methyl-1H-indol-5-yl)-1H-benzo[d]imidazol-2-yloxy)-2-methylbenzoic acid maximum activation to test compound maximum activation at 50 uM using fluorescein-labeled SAMS peptide as substrate pretreated for 30 mins in presence of AMP followed by substrate addition measured after 60 mins by fluorescence assay relative to AMP | Homo sapiens | 6 | ALA4138270 | protein complex format | Scientific Literature | |
| 18. | ALA4234637 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta2gamma1 expressed in Escherichia coli BL21 cells at 1 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | ALA4229467 | cell-based format | Scientific Literature | |
| 19. | ALA4234641 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta2gamma1 expressed in Escherichia coli BL21 cells using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay | Homo sapiens | 6 | ALA4229467 | cell-based format | Scientific Literature | |
| 20. | ALA4603802 | B | Inhibition of tracer 236 binding to recombinant human C-terminal His-tagged AMPK (A2/B2/G1) expressed in baculovirus infected Sf9 cells at 0.6 uM incubated for 1 hr by Lanthascreen assay relative to control | Homo sapiens | 1 | ALA4602634 | cell-based format | Scientific Literature |
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