| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2409987 | B | Activation of human recombinant AMPK alpha2beta2gamma3 | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
| 2. | ALA3854704 | B | Activation of recombinant human AMPK alpha2/beta2/gamma3 by using Cy5-labelled SAMS as substrate in presence of ATP by TR-FRET assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 3. | ALA3854799 | B | Activation of recombinant human AMPK alpha2/beta2/gamma3 assessed as protection from Thr172 residue dephosphorylation up to 40 uM preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 4. | ALA3859877 | B | Activation of human recombinant AMPK alpha2/beta2/gamma3 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA3859878 | B | Activation of human recombinant AMPK alpha2/beta2/gamma3 expressed in African green monkey COS7 cells assessed as increase in biotinylated AAC (1 to 120 residues) peptide phosphorylation at Ser-79 residue measured after 60 mins by Alphascreen assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4023990 | B | Allosteric activation of human AMPK alpha2/beta2/gamma3 by TR-FRET assay | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
| 7. | ALA4026644 | B | Agonist activity at human recombinant phosphorylated AMPK complex 12 alpha2/beta2/gamma3 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4026655 | B | Agonist activity at human recombinant phosphorylated AMPK complex 12 alpha2/beta2/gamma3 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate at 2 nM to 50 uM preincubated for 30 mins in presence of AMPK activator followed by substrate addition measured after 60 mins | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4144391 | B | Activation of CaMKK2-phosphorylated AMPK12 alpha2/beta2/gamma3 (unknown origin) using fluorescein-labeled SAMS peptide as substrate pretreated for 30 mins in presence of AMP followed by substrate addition measured after 60 mins by fluorescence assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 10. | ALA4234621 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta2gamma3 expressed in Escherichia coli BL21 cells using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 11. | ALA4234636 | B | Activation of full-length recombinant N-terminal His-tagged human AMPKalpha2beta2gamma3 expressed in Escherichia coli BL21 cells at 1 nM using Cy-5 SAMS peptide as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins in presence of PP2a by TR-FRET assay relative to AMP control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 12. | ALA4887239 | B | AMPK (A2/B2/G3) Invitrogen Lab (UK) kinase screen (BI) | Homo sapiens | 1 | protein complex format | Donated Chemical Probes - SGC Frankfurt |
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