| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2034661 | B | Inhibition of human tertrameric CK2alphabeta holoenzyme using casein as substrate after 1 to 2 hrs by chemiluminescence assay in presence of 2 uM ATP | Homo sapiens | 23 | protein complex format | Scientific Literature | ||
| 2. | ALA1961268 | B | Inhibition of human CSNK2A1/2B expressed in Escherichia coli BL21(DE3) using RRRDDDSDDD as substrate at 10 uM after 15 mins by scintillation counting | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 3. | ALA1961269 | B | Inhibition of human CSNK2A1/2B expressed in Escherichia coli BL21(DE3) using RRRDDDSDDD as substrate after 15 mins by scintillation counting | Homo sapiens | 20 | assay format | Scientific Literature | ||
| 4. | ALA1961270 | B | Competitive inhibition of human CSNK2A1/2B by non-radiometric capillary electrophoresis method in presence of cosubstrate ATP | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 5. | ALA1961271 | B | Competitive inhibition of human CSNK2A1/2B by Lineweaver-Burk plot analysis in presence of cosubstrate ATP | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 6. | ALA2014908 | B | Inhibition of CK2a1/b at 10 uM | 1 | protein complex format | Scientific Literature | |||
| 7. | ALA2020773 | B | Inhibition of human recombinant CK2 (alphaalpha-betabeta) holoenzyme using RRRDDDSDDD as substrate and 15 uM ATP by radiometric assay | Homo sapiens | 40 | protein complex format | Scientific Literature | ||
| 8. | ALA1114863 | B | Inhibition of CK2 alpha1/beta at 5 uM | 1 | protein complex format | Scientific Literature | |||
| 9. | ALA1105945 | B | Inhibition of CK2a1/b at 5 uM | 1 | protein complex format | Scientific Literature | |||
| 10. | ALA2444012 | B | Inhibition of CK2alpha/beta (unknown origin) | Homo sapiens | 4 | protein complex format | Scientific Literature | ||
| 11. | ALA3888220 | B | In Vitro Cell-Free Assay: In a final reaction volume of 50 μl, CK2 ααββ (4 ng, 8.5 mU) was incubated with various concentrations of test compounds in DMSO (1 ul, 2% by volume), 20 mM MOPS pH 7.2, 10 mM EGTA, 0.15 M NaCl, 10 mM DTT, 0.002% Brij-35, 200 μM RRRDDDSDDD (SEQ ID NO.: 4), 10 mM MgAcetate, ATP 15 uM and 0.33% (by volume) (hr-3311ATP: Stock 1 mCi/100 μl; 3000 Ci/mmol (Perkin Elmer)). Reactions were maintained for 40 min at 23° C. The reactions were quenched with 100 ul of 0.75% Phosphoric acid, then transferred to and filtered through a Phosphocellulose filter plate (Millipore, MSPH-N6B-50). After washing each well 4 times with 0.75% Phosphoric acid, scintillation fluid (20 uL) was added to each well and the residual radioactivity was measured using a Wallac luminescence counter. | Homo sapiens | 1,167 | protein complex format | BindingDB Database | ||
| 12. | ALA4011915 | B | Inhibition of CK2alpha1/beta (unknown origin) at 0.1 uM pretreated for 30 mins followed by substrate addition measured after 5 hrs in presence of 1 mM ATP relative to control | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 13. | ALA4035524 | B | Binding affinity to CK2alpha/beta (unknown origin) expressed in Escherichia coli BL21(DE3) by ITC | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA4035525 | B | Inhibition of CK2alpha/beta (unknown origin) expressed in Escherichia coli BL21(DE3) using RRRADDSDDDD as substrate after 40 mins by ADP-Glo assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA4224408 | B | Inhibition of CK2alpha (unknown origin) (2 to 329 residues) expressed in Escherichia coli BL21 (DE3) interaction with CK2beta (unknown origin) using fluorescein tagged RLYGFKIHPMAYQLQ probe after 10 mins by fluorescence polarization assay | Homo sapiens | 65 | assay format | Scientific Literature | ||
| 16. | ALA4224410 | B | Inhibition of [35S]methionine-labelled human recombinant His6-tagged CK2alpha/MBP-tagged CK2beta (unknown origin) interaction incubated for 1 hr by scintillation counting method | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 17. | ALA4233102 | B | Binding affinity to recombinant human CK2alpha/beta expressed in Escherichia coli by surface plasmon resonance analysis | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 18. | ALA4254194 | B | Inhibition of human CK2-alpha/beta expressed in Escherichia coli BL21(DE3) at 10 uM using RRRDDDSDDD peptide as substrate after 15 mins by capillary electrophoresis method relative to control | Homo sapiens | 31 | assay format | Scientific Literature | ||
| 19. | ALA4254195 | B | Inhibition of human CK2-alpha/beta expressed in Escherichia coli BL21(DE3) using RRRDDDSDDD peptide as substrate after 15 mins by capillary electrophoresis method | Homo sapiens | 29 | assay format | Scientific Literature | ||
| 20. | ALA4254198 | B | ATP-competitive inhibition of human CK2-alpha/beta expressed in Escherichia coli BL21(DE3) using RRRDDDSDDD peptide as substrate after 15 mins in presence of varying levels of ATP by capillary electrophoresis method | Homo sapiens | 1 | assay format | Scientific Literature |
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