#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2020637	B	Inhibition of beta-casein/TCF4 pathway expressed in HCT116 cells containing delta45S mutant after 24 hrs by luciferase reporter gene assay		20		cell-based format	Scientific Literature
2.	ALA1919781	B	Inhibition of GST-tagged T cell factor4/beta casein interaction assessed as reduction in alkaline phosphatase level		1		cell-based format	Scientific Literature
3.	ALA2328061	B	Inhibition of human beta-casein/Tcf4 interaction after 1 hr by fluorescence polarization assay	Homo sapiens	7		protein complex format	Scientific Literature
4.	ALA2328064	B	Inhibition of human beta-casein/Tcf4 interaction after 1 hr by alphascreen assay	Homo sapiens	7		protein complex format	Scientific Literature
5.	ALA2353389	B	Inhibition of transcription activity of beta-casein/TCF4 (unknown origin) expressed in HEK293 cells after 24 hrs by luciferase reporter gene assay	Homo sapiens	1		cell-based format	Scientific Literature
6.	ALA3881537	B	Inhibition of TCF-4 ( 1 to 56 residues) (unknown origin) binding to beta-catenin/armadillo (unknown origin) at 50 uM by ITC assay	Homo sapiens	39		protein complex format	Patent Bioactivity Data
7.	ALA3881538	B	Inhibition of TCF-4 ( 1 to 56 residues) (unknown origin) binding to beta-catenin/armadillo (unknown origin) by ITC assay	Homo sapiens	1		protein complex format	Patent Bioactivity Data
8.	ALA3881539	B	Inhibition of TCF-4 ( 1 to 56 residues) (unknown origin) binding to beta-catenin/armadillo (unknown origin) assessed as dissociation constant by ITC assay	Homo sapiens	1		protein complex format	Patent Bioactivity Data
9.	ALA3881540	B	Inhibition of TCF-4 ( 1 to 56 residues) (unknown origin) binding to beta-catenin/armadillo (unknown origin) at 50 uM by ITC assay relative to control	Homo sapiens	1		protein complex format	Patent Bioactivity Data
10.	ALA3881541	B	Antagonist activity against TCF-4 ( 1 to 56 residues) (unknown origin) binding to beta-catenin armadillo repeat units (unknown origin) assessed as reduction in TCF4-beta-catenin binding at 50 uM at 20 degC by WaterLOGSY NMR screening method	Homo sapiens	1		protein complex format	Patent Bioactivity Data
11.	ALA3883063	B	Inhibition of TCF-4/beta catenin in human Tcf33.13 cells expressing Tcf-4/SV40 binding sites driving firefly luciferase reporter gene after 24 hrs by luminometer	Homo sapiens	264		cell-based format	Patent Bioactivity Data
12.	ALA3883064	B	Inhibition of TCF-4/beta catenin in human Tcf22C11 cells expressing Tcf-4/SV40 binding sites driving firefly luciferase reporter gene after 24 hrs by luminometer	Homo sapiens	264		cell-based format	Patent Bioactivity Data
13.	ALA2438919	B	Inhibition of human TCF4/ flag epitope-tagged beta-catenin (unknown origin) interaction transfected in human HCT116 cells after 24 hrs by beta-galactosidase/luciferase reporter gene assay	Homo sapiens	1		cell-based format	Scientific Literature
14.	ALA3267625	B	Inhibition of beta-catenin/T cell factor (unknown origin)	Homo sapiens	1		protein complex format	Scientific Literature
15.	ALA3888145	B	AlphaScreen Assay: Experiments were performed in white opaque 384-well plates from PerkinElmer (Waltham, Mass.), and the samples were read on a Synergy 2 plate reader (Biotek, Winooski, Vt.) with a sensitivity setting of 200 using AlphaScreen protocol with excitation at 680 nm and emission at 570 nm. All dilutions were made in 1× assay buffer containing 25 mM HEPES (pH 7.4), 100 mMNaCl and 0.1% BSA to minimize nonspecific interactions. For inhibitor competition experiment, 5 nM of C-terminal biotinylated Tcf4 45-mer, 20 nM of N-terminal His6-tagged β-catenin and inhibitors at different concentration were incubated in 20 μL of assay buffer for 2 h. Donor and acceptor beads were added to a final concentration of 10 μg/mL in 25 μL of assay buffer. The mixture was incubated at room temperature for 1 h. For each inhibitor competition assay, the negative controls (equivalent to 0% inhibition) refer to 5.0 nM of biotinylated Tcf4 45-mer, 20 nM of β-catenin, 10 μg/mL donor and acce		3		protein complex format	BindingDB Database
16.	ALA3888148	B	Fluorescence Polarization (FP) Assay: Experiments were performed in 96-well Microfluor 2 black plates (Waltham, Mass.), and the samples were read by a Synergy 2 plate reader (Biotek, Winooski, Vt.). The fluorescence polarization (FP) was measured at room temperature with an excitation wavelength at 485 nm and an emission wavelength at 535 nm. The FP saturation experiment was performed in an assay buffer of 137 mM of NaCl, 2.7 mM of KCl, 10 mM of Na2HPO4, 2 mM of KH2PO4, 100 ug/mL of bovine gamma globulin, and 0.01% Triton-X 100. The final reaction volume is 100 uL. The competitive FP assays used 2.5 nM of Tcf4 fluorescence tracer, 10 nM of β-catenin, and different concentrations of the tested inhibitors in 100 uL of assay buffer. Each assay plate was covered black and gently mixed on an orbital shaker for 3 h to reach equilibrium before polarization values were read. For each inhibitor competition assay, the negative controls (equivalent to 0% inhibition) refer to 2.5 nM of Tcf4 fluorescence tracer and 10 nM of β-catenin, but no tested peptide inhibitor was presented. The positive controls (equivalent to 100% inhibition) refer to only 2.5 nM of Tcf4 fluorescence tracer in a final volume of 100 uL.		4		protein complex format	BindingDB Database