| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3111725 | B | Inhibition of WHSC1 (unknown origin)-mediated incorporation of methyl group from [3H]-SAM into peptide substrate up to 50 uM after 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA3108684 | single protein format | Scientific Literature | |
| 2. | ALA3214944 | B | PubChem BioAssay. Development of Small Molecule Probes of the Histone Methyltransferase, NSD2 Measured in Biochemical System Using Plate Reader - 7053-01_Inhibitor_Dose_CherryPick_Activity. (Class of assay: confirmatory) | 578 | ALA1201862 | biochemical format | PubChem BioAssays | ||
| 3. | ALA3592412 | B | Inhibition of NSD2 (unknown origin) at 1 uM after 1 hr by filter-based assay | Homo sapiens | 1 | ALA3588844 | single protein format | Scientific Literature | |
| 4. | ALA3784090 | B | Inhibition of human NSD2 at 10 and 50 uM | Homo sapiens | 1 | ALA3779843 | single protein format | Scientific Literature | |
| 5. | ALA3803386 | B | Inhibition of WHSC1 (unknown origin) at 10 uM relative to control | Homo sapiens | 2 | ALA3797202 | single protein format | Scientific Literature | |
| 6. | ALA3821915 | B | Inhibition of NSD2 (unknown origin) at 1 to 50 uM by TCA precipitation assay | Homo sapiens | 1 | ALA3817823 | single protein format | Scientific Literature | |
| 7. | ALA3859063 | B | Inhibition of NSD2 (unknown origin) at 50 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA3856251 | single protein format | Scientific Literature | |
| 8. | ALA4266989 | B | Inhibition of full-length NSD2 (unknown origin) using HeLa oligonucleosome as substrate preincubated for 15 mins followed by substrate addition measured after 45 mins in presence of SAM by MTaseGlo assay | Homo sapiens | 13 | ALA4265895 | single protein format | Scientific Literature | |
| 9. | ALA4306967 | B | Inhibition of N-terminal GST-tagged human NSD2 catalytic domain (941 to 1240 amino acids) expressed in Escherichia coli assessed as enzyme remaining activity at 50 uM pre-incubated for 15 mins followed by AdoMet addition and measured after 1 hr by luminescence method relative to control | Homo sapiens | 1 | ALA4304774 | assay format | Scientific Literature | |
| 10. | ALA4346639 | B | Inhibition of NSD2 (unknown origin) at 10 uM using [3H-SAM] and nucleosome as substrate incubated for 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA4342528 | single protein format | Scientific Literature | |
| 11. | ALA4377328 | B | Inhibition of human NSD2 up to 50 uM using chicken nucleosome as substrate and in presence of [3H]-SAM incubated for 1 hr by filter-based assay | Homo sapiens | 1 | ALA4376809 | single protein format | Scientific Literature | |
| 12. | ALA4379083 | B | Binding affinity to NSD2 (unknown origin) at protein to compound concentration ratio of 1:20 by differential scanning fluorimetry | Homo sapiens | 1 | ALA4376834 | single protein format | Scientific Literature | |
| 13. | ALA4398078 | B | Inhibition of NSD2 (unknown origin) up to 50 uM preincubated for 15 mins followed by substrate addition and measured after 60 mins by AlphaLisa assay | Homo sapiens | 1 | ALA4396888 | single protein format | Scientific Literature | |
| 14. | ALA4480239 | B | Inhibition of NSD2 (unknown origin) using biotin-labeled histone substrate assessed as residual activity at 50 uM in presence of [3H-Me]SAM by scintillation proximity assay relative to control | Homo sapiens | 24 | ALA4477270 | single protein format | Scientific Literature | |
| 15. | ALA4628258 | B | Inhibition of NSD2 (unknown origin) preincubated for 15 mins followed by addition of [3H]-SAM as substrate and peptide solution after 240 mins cold SAM was added measured by radioisotope assay | Homo sapiens | 1 | ALA4627299 | single protein format | Scientific Literature | |
| 16. | ALA4736934 | B | Binding affinity to NSD2 PWWP1 domain (unknown origin) assessed as change in aggregation temperature at 400 uM by heating at 25 to 85 degC by differential static light scattering method | Homo sapiens | 1 | ALA4732170 | single protein format | Scientific Literature | |
| 17. | ALA4736935 | B | Binding affinity to NSD2 PWWP1 domain (unknown origin) assessed as dissociation constant by surface plasmon resonance analysis | Homo sapiens | 19 | ALA4732170 | single protein format | Scientific Literature | |
| 18. | ALA4736936 | B | Binding affinity to NSD2 PWWP1 domain (unknown origin) assessed as dissociation constant by isothermal titration calorimetric analysis | Homo sapiens | 1 | ALA4732170 | single protein format | Scientific Literature | |
| 19. | ALA4736943 | B | Binding affinity to NSD2 PWWP1 domain (unknown origin) assessed as change in aggregation temperature at 500 uM by heating at 25 to 85 degC by differential static light scattering method | Homo sapiens | 18 | ALA4732170 | single protein format | Scientific Literature | |
| 20. | ALA4736944 | B | Displacement of Halo-tagged Histone H3.3 from Nanoluc-tagged NSD2- PWWP1 (unknown origin) transfected in human U2OS cells preincubated for one day followed by Nano-GloR substrate addition at the next day and measured withing 10 mins by NanoBRET assay | Homo sapiens | 1 | ALA4732170 | cell-based format | Scientific Literature |
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