| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3110591 | B | Displacement of 19-mer HIF-1alpha peptide from VHL (unknown origin) by isothermal titration calorimetry analysis | Homo sapiens | 8 | single protein format | Scientific Literature | ||
| 2. | ALA3110592 | B | Binding affinity to VHL (unknown origin) at 3 mM using 30 uM of protein by NMR-WaterLOGSY analysis | Homo sapiens | 12 | single protein format | Scientific Literature | ||
| 3. | ALA3110593 | B | Binding affinity to VHL (unknown origin) at 1 mM using 40 uM of protein by NMR-CPMG analysis | Homo sapiens | 12 | single protein format | Scientific Literature | ||
| 4. | ALA3110594 | B | Binding affinity to VHL (unknown origin) at 1 mM using 10 uM of protein by NMR-STD analysis | Homo sapiens | 12 | single protein format | Scientific Literature | ||
| 5. | ALA3118409 | B | Inhibition of 6xHis-tagged VHL (unknown origin) expressed in Escherichia coli BL21 cells by fluorescence polarization assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3370433 | B | Binding affinity to N-terminal His6-tagged pVHL (54 to 213 amino acids) (unknown origin) expressed in Escherichia coli BL21 (DE3) by isothermal titration calorimetry | Homo sapiens | 17 | assay format | Scientific Literature | ||
| 7. | ALA3705901 | B | Inhibition Assay: Hydroxylated HIF bonds specifically to the von Hippel-Lindau protein-elongin B-elongin C complex (VBC complex). This interaction occurs only if HIF is hydroxylated on a conserved prolyl radical. It is the basis for the biochemical determination of HIF prolyl hydroxylase activity. The test is carried out as described [Oehme F., Jonghaus W., Narouz-Ott L., Huetter J., Flamme I., Anal. Biochem. 330 (1), 74-80 (2004)]:A clear 96-well microtiter plate coated with NeutrAvidin HBC (Pierce) is incubated with blocker casein for 30 minutes. The plate is then washed three times with 200 ul each time of wash buffer (50 mM Tris, pH 7.5, 100 mM NaCl, 10% (v/v) blocker casein, 0.05% (v/v) Tween 20) per well. The peptide biotin-DLDLEMLAPYIPMDDDFQL (Eurogentec, 4102 Seraing, Belgium) is added in a concentration of 400 nM in 100 ul wash buffer. This peptide serves as a substrate for the prolyl hydroxylation and is bonded to the microtiter plate. | 10 | single protein format | BindingDB Database | |||
| 8. | ALA4026935 | B | Binding affinity to VHL (unknown origin) assessed as reduction of HIF1alpha derived peptide binding to VHL complex by competitive binding fluorescence polarization assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 9. | ALA4349408 | B | Binding affinity to VHL in human THP-1 cells | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4396993 | B | Inhibition of VHL (unknown origin) by fluorescence polarization assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 11. | ALA4396995 | B | Binding affinity to VHL (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 12. | ALA4620018 | B | Inhibition of VHL (unknown origin) by fluorescence polarization assay | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 13. | ALA4724695 | B | Inhibition of NanoLuc-tagged VHL in HEK293 cells incubated for 2 hrs by NanoBRET live cell assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4724700 | B | Inhibition of VHL (unknown origin) assessed as phosphorylated peptide substrate incubated for 60 mins by TR-FRET based biochemical assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4730314 | B | Displacement of FAM-labeled HIF-1alpha peptide from VCB (unknown origin) by fluorescence polarization assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 16. | ALA5029482 | B | Displacement of 5-FAM-labelled DEALA-HypYIPMDDDFQLRSF peptide from VHL (unknown origin) by fluorescence polarization assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 17. | ALA5036171 | B | Binding affinity to N-terminal His6-tagged VHL (54 to 213 residues) (unknown origin) expressed in Escherichia coli assessed as displacement of HIF-1alpha by fluorescence polarization assay | Homo sapiens | 9 | assay format | Scientific Literature | ||
| 18. | ALA5036179 | B | Binding affinity to N-terminal His6-tagged VHL (54 to 213 residues) (unknown origin) expressed in Escherichia coli assessed as displacement of HIF-1alpha by binary binding competitive fluorescence polarization assay | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 19. | ALA5054773 | B | Binding affinity to VHL (unknown origin) assessed as displacement of HIF-1alpha by binary binding competitive fluorescence polarization assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 20. | ALA5153514 | B | Binding affinity to VHL in human A2780 cells assessed as change in melting temperature at 10 uM after 2 hrs by CESTA | Homo sapiens | 1 | cell-based format | Scientific Literature |
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