#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3364950	Binding	Induction of GK translocation from nucleus to cytoplasm of mouse hepatocytes preincubated for 20 mins followed by glucose challenge measured after 40 mins by Hoechst 33342 staining-based assay	Mus musculus	40	ALA3352455	cell-based format	Scientific Literature
2.	ALA3119630	Binding	Induction of GK translocation from nucleus to cytoplasm of mouse hepatocytes preincubated for 20 mins followed by glucose challenge measured after 40 mins by Hoechst 33342 staining-based assay	Mus musculus	30	ALA3112561	cell-based format	Scientific Literature
3.	ALA3888820	Binding	Activation Assay: The final assay volume was 200 uL and the final buffer conditions used in this assay were as follows: 25 mM HEPES, 5 mM glucose, 1 mM ATP, 2 mM MgCl2, 1 mM NAD, 1 mM DTT, 8.5 U/mL G6PDH, 100 nM glucokinase, and 25 mM KCl. The buffer pH was 7.1. The mixture A was first made containing KCl, MgCl2, DTT and Glucose in HEPES buffer. The mixture B was made containing NAD and ATP. The test compound in DMSO solution, the mixture A, the mixture B and G6PDH were first mixed in a 96-well plate. Glucokinase was then added to initiate the reaction, and the absorbance at 340 nm was monitored every 5 mins. The activity of glucokinase was represented by the initial generation rate of glucose-6-phosphate, which was calculated by drawing the slope values from the absorbance changing curve at 340 nm vs. the time points. Compounds of this invention have an EC50 value as measured using the assay described herein of less than 50 μM.		4	ALA3886848	single protein format	BindingDB Database
4.	ALA4272650	Binding	Activation of glucokinase in mouse MIN6 cells assessed as increase in insulin secretion at 10 uM after 45 mins by ELISA relative to RO0281675	Mus musculus	14	ALA4270548	cell-based format	Scientific Literature
5.	ALA5048929	Binding	Activation of recombinant mouse full length liver glucokinase expressed in Escherichia coli incubated for 10 mins in presence of 12 mM glucose by plate reader analysis	Mus musculus	2	ALA5046279	tissue-based format	Scientific Literature
6.	ALA5048930	Binding	Activation of recombinant mouse full length liver glucokinase expressed in Escherichia coli assessed as maximal activation incubated for 10 mins in presence of 12 mM glucose by plate reader analysis relative to control	Mus musculus	2	ALA5046279	tissue-based format	Scientific Literature