| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3111898 | B | Inhibition of PRMT5/MEP50 complex (unknown origin)-mediated incorporation of methyl group from [3H]-SAM into peptide substrate up to 50 uM by scintillation proximity assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 2. | ALA3578929 | B | Inhibition of human full length PRMT5-MEP50 complex expressed in Sf9 cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 3. | ALA3578930 | B | Inhibition of human full length PRMT5-MEP50 complex expressed in Sf9 cells assessed as inhibition of methylation of peptide substrate at 750 uM | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA3578936 | B | Binding affinity to human full length PRMT5-MEP50 complex by Surface plasmon resonance method | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 5. | ALA3578938 | B | Noncompetitive inhibition of human full length PRMT5-MEP50 complex expressed in Sf9 cells in presence of increasing concentration of histone peptide substrate | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3578947 | B | Inhibition of human full length PRMT5-MEP50 complex expressed in Sf9 cells using SmD3 peptide AGRGRGKAAILKAQVAARGRGRGMGRGN as a substrate | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA3578949 | B | Inhibition of human full length PRMT5-MEP50 complex expressed in Sf9 cells using full length histone H4 substrate | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3592407 | B | Inhibition of PRMT5-MEP50 complex (unknown origin) assessed as incorporation of tritium labeled methyl group from [3H]-SAM to substrate at 1 uM after 1 hr by scintillation proximity assay relative to control | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 9. | ALA3880377 | B | Inhibition of recombinant human fetal brain FLAG-tagged PRMT5/full length human testis 6His-tagged MEP50 expressed in baculovirus infected insect Sf9 cells using human histone H4 (1 to 15 residues) as substrate preincubated for 30 mins followed by [3H]-SAM addition | Homo sapiens | 562 | cell-based format | Patent Bioactivity Data | ||
| 10. | ALA4005626 | B | Inhibition of PRMT5/MEP50 complex (unknown origin) at 10 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 11. | ALA4005655 | B | Inhibition of PRMT5/MEP50 complex (unknown origin) at 1 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 12. | ALA4013545 | B | Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA assay | Homo sapiens | 37 | cell-based format | Scientific Literature | ||
| 13. | ALA4013546 | B | Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues)/human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using histone H4 as substrate in presence of [3H]-SAM after 60 mins by chemiluminescent assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 14. | ALA4013576 | B | Binding affinity to PRMT5/MEP50 (unknown origin) by surface plasmon resonance assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 15. | ALA4013581 | B | Competitive inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using peptide substrate preincubated for 15 mins followed by substrate/[3H]-SAM addition measured after 60 mins under varying concentration of SAM | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4013582 | B | Non-competitive inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using peptide substrate preincubated for 15 mins followed by substrate/[3H]-SAM addition measured after 60 mins under varying concentration of peptide | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4013583 | B | Competitive inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using peptide substrate preincubated for 15 mins followed by substrate/[3H]-SAM addition measured after 60 mins under varying concentration of peptide | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA4013584 | B | Uncompetitive inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using peptide substrate preincubated for 15 mins followed by substrate/[3H]-SAM addition measured after 60 mins under varying concentration of SAM | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA4121324 | B | Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells at 100 uM using substrate pretreated for 15 mins followed by substrate and [3H]-SAM addition measured after 60 mins by scintillation proximity assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 20. | ALA4121325 | B | Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using substrate pretreated for 15 mins followed by substrate and [3H]-SAM addition measured after 60 mins by scintillation proximity assay | Homo sapiens | 7 | cell-based format | Scientific Literature |
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