# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3110656 | B | Inhibition of recombinant human LSD1/CoREST complex expressed in Escherichia coli using methylated H3-K4 peptide as substrate by peroxidase-coupled assay | Homo sapiens | 1 | ALA3108747 | assay format | Scientific Literature | |
2. | ALA3411111 | B | Inhibition of human recombinant KDM1A/CoREST complex expressed in Escherichia coli using mono-methylated H3-K4 peptide containing 21 amino acids as substrate preincubated for 15 mins followed by substrate addition measured for 12 mins by fluorescence assay | Homo sapiens | 28 | ALA3407462 | assay format | Scientific Literature | |
3. | ALA3411112 | B | Inhibition of human recombinant KDM1A/CoREST complex expressed in Escherichia coli using mono-methylated H3-K4 peptide containing 21 amino acids as substrate up to 100 uM preincubated for 15 mins followed by substrate addition measured for 12 mins by fluorescence assay | Homo sapiens | 2 | ALA3407462 | assay format | Scientific Literature | |
4. | ALA3626334 | B | Inhibition of recombinant LSD1/CoREST (unknown origin) assessed as residual activity at 10 uM incubated for 30 mins to 4 hrs by fluorescence based assay relative to control | Homo sapiens | 33 | ALA3621154 | protein complex format | Scientific Literature | |
5. | ALA3626335 | B | Inhibition of recombinant LSD1/CoREST (unknown origin) assessed as residual activity for 30 mins to 4 hrs by fluorescence based assay relative to control | Homo sapiens | 3 | ALA3621154 | protein complex format | Scientific Literature | |
6. | ALA3737230 | B | Inhibition of human recombinant LSD1/CoREST protein expressed in Escherichia coli using monomethylated H3-K4 peptide (21 amino acids) as substrate preincubated for 15 mins followed by substrate addition measured for 12 mins by HRP-coupled Ultrared detection system-based fluorescence analysis | Homo sapiens | 15 | ALA3734732 | assay format | Scientific Literature | |
7. | ALA3737231 | B | Potency index, ratio of trans-benzyl 1-(4-(2-aminocyclopropyl)phenylamino)-1-oxo-3-phenylpropan-2-ylcarbamate hydrochloride IC50 to compound IC50 for human recombinant LSD1/CoREST protein expressed in Escherichia coli | Homo sapiens | 7 | ALA3734732 | assay format | Scientific Literature | |
8. | ALA3737241 | B | Inhibition of human recombinant LSD1/CoREST protein expressed in Escherichia coli using monomethylated H3-K4 peptide as substrate by peroxidase-coupled assay | Homo sapiens | 1 | ALA3734732 | assay format | Scientific Literature | |
9. | ALA3737242 | B | Competitive inhibition of human recombinant LSD1/CoREST protein expressed in Escherichia coli using monomethylated H3-K4 peptide as substrate | Homo sapiens | 1 | ALA3734732 | assay format | Scientific Literature | |
10. | ALA3782872 | B | Inhibition of human recombinant KDM1A/CoREST expressed in Escherichia coli using mono-methylated H3-K4 peptide as substrate assessed as H2O2 release preincubated for 15 mins followed by substrate addition measured for 12 mins by fluorescence-based microplate reader analysis | Homo sapiens | 14 | ALA3779820 | assay format | Scientific Literature | |
11. | ALA3877045 | B | Inhibition of recombinant human His-tagged LSD1 (171 to 836 residues)/GST-tagged CoREST (308 to 440 residues) complex using H3K4 peptide substrate by spectrophotometric method | Homo sapiens | 1 | ALA3875223 | protein complex format | Scientific Literature | |
12. | ALA4008765 | B | Inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using biotinylated H3K4me1 peptide as substrate preincubated for 10 mins with enzyme followed by substrate addition measured after 1 hr by TR-FRET HTS assay | Homo sapiens | 4 | ALA4007415 | assay format | Scientific Literature | |
13. | ALA4008766 | B | Inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using biotinylated H3K4me1 peptide as substrate preincubated for 20 mins measured after 1 hr by TR-FRET assay | Homo sapiens | 59 | ALA4007415 | assay format | Scientific Literature | |
14. | ALA4008767 | B | Inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using H3K4me1 peptide as substrate preincubated for 15 mins with enzyme followed by substrate addition measured after 12 mins by Amplex Ultra Red reagent based HRP enzyme coupled assay | Homo sapiens | 3 | ALA4007415 | assay format | Scientific Literature | |
15. | ALA4008768 | B | Irreversible inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using H3K4me1 peptide as substrate preincubated for 15 mins with enzyme followed by substrate addition by jump dilution based HRP enzyme coupled assay | Homo sapiens | 1 | ALA4007415 | assay format | Scientific Literature | |
16. | ALA4008769 | B | Reversible inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using H3K4me1 peptide as substrate preincubated for 15 mins with enzyme followed by substrate addition by jump dilution based HRP enzyme coupled assay | Homo sapiens | 2 | ALA4007415 | assay format | Scientific Literature | |
17. | ALA4008770 | B | Non-competitive inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using H3K4me1 peptide as substrate preincubated for 15 mins followed by substrate addition measured for 12 mins by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4007415 | assay format | Scientific Literature | |
18. | ALA4008771 | B | Competitive inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using H3K4me1 peptide as substrate preincubated for 15 mins followed by substrate addition measured for 12 mins by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4007415 | assay format | Scientific Literature | |
19. | ALA4008775 | B | Inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli assessed as Vmax for substrate using H3K4me1 as substrate preincubated for 15 mins followed by substrate addition measured for 12 mins by Lineweaver-Burk plot analysis | Homo sapiens | 2 | ALA4007415 | assay format | Scientific Literature | |
20. | ALA4008776 | B | Inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli assessed as Km for substrate using H3K4me1 peptide as substrate preincubated for 15 mins followed by substrate addition measured for 12 mins by Lineweaver-Burk plot analysis | Homo sapiens | 2 | ALA4007415 | assay format | Scientific Literature |