#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3266690	B	Inhibition of Escherichia coli topoisomerase 1-mediated pHOT-1 DNA relaxation treated with pHOT-1 substrate for 15 mins prior to enzyme addition for 30 mins by agarose gel electrophoresis	Escherichia coli	2		single protein format	Scientific Literature
2.	ALA3266691	B	Inhibition of Escherichia coli topoisomerase 1-mediated pHOT-1 DNA relaxation at >90 uM treated with pHOT-1 substrate for 15 mins prior to enzyme addition for 30 mins by agarose gel electrophoresis	Escherichia coli	1		single protein format	Scientific Literature
3.	ALA3266693	B	Inhibition of Escherichia coli topoisomerase 1 assessed as single stranded breaks using pHOT-1 DNA as substrate treated with substrate for 15 mins prior to enzyme addition for 30 mins by agarose gel electrophoresis	Escherichia coli	1		single protein format	Scientific Literature
4.	ALA4380659	B	Inhibition of Escherichia coli Top1 assessed as decrease in relaxation of supercoiled pBR322 plasmid DNA at 100 uM using supercoiled pBR322 plasmid DNA as substrate incubated for 15 mins by agarose gel electrophoresis analysis	Escherichia coli	4		single protein format	Scientific Literature
5.	ALA4380713	B	Poison activity at Escherichia coli Top1 assessed as increase in nickel band intensity at 12.5 to 100 uM using supercoiled pUC19 plasmid DNA as substrate incubated for 30 mins by agarose gel electrophoresis analysis	Escherichia coli	1		single protein format	Scientific Literature
6.	ALA4380714	B	Poison activity at Escherichia coli Top1 assessed as increase in nickel band intensity at 100 uM using supercoiled pUC19 plasmid DNA as substrate incubated for 30 mins by agarose gel electrophoresis analysis	Escherichia coli	1		single protein format	Scientific Literature
7.	ALA4386608	B	Inhibition of Escherichia coli topoisomerase 1 mediated DNA relaxation at 10 uM using plasmid pMT-puro DNA as substrate after 30 mins by ethidium bromide staining-based agarose gel electrophoresis method	Escherichia coli	7		single protein format	Scientific Literature
8.	ALA4386609	B	Inhibition of Escherichia coli topoisomerase 1 mediated DNA relaxation at 1 uM using plasmid pMT-puro DNA as substrate after 30 mins by ethidium bromide staining-based agarose gel electrophoresis method	Escherichia coli	1		single protein format	Scientific Literature
9.	ALA4386610	B	Inhibition of Escherichia coli topoisomerase 1 mediated DNA relaxation using plasmid pMT-puro DNA as substrate after 30 mins by ethidium bromide staining-based agarose gel electrophoresis method	Escherichia coli	1		single protein format	Scientific Literature