# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3293898 | B | Binding affinity to human recombinant CXCL12 by surface plasmon resonance assay | Homo sapiens | 3 | ALA3286317 | single protein format | Scientific Literature | |
2. | ALA3374590 | B | Binding affinity to CXCL12 in human THP1 cells assessed as inhibition of CXCL12-induced cell migration at 25 uM incubated for 2 hrs by flow cytometry based chemotaxis assay | Homo sapiens | 2 | ALA3351333 | cell-based format | Scientific Literature | |
3. | ALA3820795 | B | Binding affinity to His6-SUMO fusion tagged wild type human [U-15N]CXCL12 expressed in Escherichia coli assessed as induction of chemical shift perturbations to residues at I4/I6 binding site by 2D 1H-15N HMQC NMR spectroscopy | Homo sapiens | 7 | ALA3817737 | assay format | Scientific Literature | |
4. | ALA3820796 | B | Binding affinity to His6-SUMO fusion tagged wild type human [U-15N]CXCL12 expressed in Escherichia coli by 2D 1H-15N HMQC NMR spectroscopy | Homo sapiens | 5 | ALA3817737 | assay format | Scientific Literature | |
5. | ALA3820797 | B | Binding affinity to human [U-15N]CXCL12 L36C/A65C dimer mutant expressed in Escherichia coli by 2D 1H-15N HMQC NMR spectroscopy | Homo sapiens | 1 | ALA3817737 | assay format | Scientific Literature | |
6. | ALA3820798 | B | Binding affinity to human [U-15N]CXCL12 L36C/A65C dimer mutant expressed in Escherichia coli assessed as induction of chemical shift perturbations at Y7/K24/K27/I28/N30/I38/V39/A40/Q48 residues by 2D 1H-15N SOFAST HMQC NMR spectroscopy | Homo sapiens | 1 | ALA3817737 | assay format | Scientific Literature | |
7. | ALA3820799 | B | Binding affinity to human [U-15N]CXCL12 L36C/A65C dimer mutant expressed in Escherichia coli assessed as induction of chemical shift perturbations at C11/T31/Q37/V49 residues by 2D 1H-15N SOFAST HMQC NMR spectroscopy | Homo sapiens | 1 | ALA3817737 | assay format | Scientific Literature | |
8. | ALA3820800 | B | Binding affinity to human [U-15N]CXCL12 L36C/A65C dimer mutant expressed in Escherichia coli assessed as induction of chemical shift perturbations at W57 residues by 2D 1H-15N SOFAST HMQC NMR spectroscopy | Homo sapiens | 2 | ALA3817737 | assay format | Scientific Literature | |
9. | ALA3820801 | B | Binding affinity to human [U-15N]CXCL12 L36C/A65C dimer mutant expressed in Escherichia coli by 2D 1H-15N SOFAST HMQC NMR spectroscopy | Homo sapiens | 1 | ALA3817737 | assay format | Scientific Literature | |
10. | ALA3820802 | B | Binding affinity to human [U-15N]CXCL12 L36C/A65C dimer mutant expressed in Escherichia coli assessed as induction of chemical shift perturbations at Y61 residues by 2D 1H-15N SOFAST HMQC NMR spectroscopy | Homo sapiens | 1 | ALA3817737 | assay format | Scientific Literature | |
11. | ALA3820803 | B | Binding affinity to human [U-15N]CXCL12 L36C/A65C dimer mutant expressed in Escherichia coli assessed as induction of chemical shift perturbations at sY12 binding site by 2D 1H-15N SOFAST HMQC NMR spectroscopy | Homo sapiens | 1 | ALA3817737 | assay format | Scientific Literature | |
12. | ALA4151994 | B | Binding affinity to Texas Red-labelled CXCL12 (unknown origin) assessed as inhibition of CXCL12-Texas Red binding to EGFP-labeled human CXCR4 expressed in HEK293 cell membranes pre-incubated for 1 hr by FRET-based binding assay | Homo sapiens | 47 | ALA4145692 | cell-based format | Scientific Literature | |
13. | ALA4151995 | B | Binding affinity to Texas Red-labelled CXCL12 (unknown origin) assessed as inhibition of CXCL12-Texas Red binding to EGFP-labeled human CXCR4 expressed in HEK293 cell membranes at 30 uM pre-incubated for 1 hr by FRET-based binding assay | Homo sapiens | 4 | ALA4145692 | cell-based format | Scientific Literature | |
14. | ALA4151996 | B | Binding affinity to Texas Red-labelled CXCL12 (unknown origin) assessed as inhibition of CXCL12-Texas Red binding to EGFP-labeled human CXCR4 expressed in HEK293 cell membranes at 10 uM pre-incubated for 1 hr by FRET-based binding assay | Homo sapiens | 2 | ALA4145692 | cell-based format | Scientific Literature | |
15. | ALA4151998 | B | Binding affinity to Texas Red-labelled CXCL12 (unknown origin) assessed as inhibition of CXCL12-Texas Red binding to EGFP-labeled human CXCR4 expressed in HEK293 cell membranes at 1 uM pre-incubated for 1 hr by FRET-based binding assay | Homo sapiens | 1 | ALA4145692 | cell-based format | Scientific Literature | |
16. | ALA4151999 | B | Binding affinity to Texas Red-labelled CXCL12 (unknown origin) assessed as inhibition of CXCL12-Texas Red binding to EGFP-labeled human CXCR4 expressed in HEK293 cell membranes at 5 uM pre-incubated for 1 hr by FRET-based binding assay | Homo sapiens | 2 | ALA4145692 | cell-based format | Scientific Literature | |
17. | ALA4152000 | B | Binding affinity to Texas Red-labelled CXCL12 (unknown origin) assessed as inhibition of CXCL12-Texas Red binding to EGFP-labeled human CXCR4 expressed in HEK293 cell membranes at 0.3 uM pre-incubated for 1 hr by FRET-based binding assay | Homo sapiens | 1 | ALA4145692 | cell-based format | Scientific Literature | |
18. | ALA4152010 | B | Binding affinity to CXCL12 (unknown origin) assessed as changes in tryptophan fluorescence intensity | Homo sapiens | 2 | ALA4145692 | single protein format | Scientific Literature | |
19. | ALA4152011 | B | Binding affinity to Texas Red-labelled CXCL12 (unknown origin) assessed as inhibition of CXCL12-Texas Red binding to EGFP-labeled human CXCR4 expressed in HEK293 cell membranes at 300 uM pre-incubated for 1 hr by FRET-based binding assay | Homo sapiens | 1 | ALA4145692 | cell-based format | Scientific Literature | |
20. | ALA4152012 | B | Binding affinity to CXCL12 (unknown origin) assessed as inhibition of CXCL12 -induced increase in intracellular calcium level in EGFP-tagged CXCR4 expressing HEK cells at 10 uM | Homo sapiens | 1 | ALA4145692 | cell-based format | Scientific Literature |