| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3267622 | B | Displacement of FAM-DEALAHyp-YIPD from 6xHis-tagged VHL (1 to 213)/elongins-B/elonginC (unknown origin) expressed in Escherichia coli BL21 after 1 min by fluorescence polarization assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 2. | ALA3267623 | B | Displacement of FAM-DEALAHyp-YIPD from VHL/elongins-B/elonginC (unknown origin) by isothermal titration calorimetric analysis | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 3. | ALA3267624 | B | Displacement of FAM-DEALAHyp-YIPD from VHL/elongins-B/elonginC (unknown origin) by fluorescence polarization assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 4. | ALA4219528 | B | Binding affinity to N-terminal His6-tagged recombinant human VHL (54 to 213 residues)/elongin C (17 to 112 residues)/elongin B (1 to 104 residues) expressed in Escherichia coli BL21 (DE3) assessed as compound binary complex formation by isothermal titration calorimetry-based assay | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 5. | ALA4219880 | B | Inhibition of VBC in human HeLa cells expressing HRE-luciferase reporter gene assessed as reduction in HIF transcriptional activity at 1 to 250 uM after 24 hrs by luciferase reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4219881 | B | Displacement of FAM-labeled HIF-1alpha peptide from VBC (unknown origin) by fluorescence polarization assay | Homo sapiens | 21 | protein complex format | Scientific Literature | ||
| 7. | ALA4219882 | B | Binding affinity to VHL (unknown origin)/elongin C (unknown origin)/elongin B (unknown origin) by isothermal titration calorimetry-based assay | Homo sapiens | 9 | protein complex format | Scientific Literature | ||
| 8. | ALA4219883 | B | Inhibition of VBC in human HeLa cells assessed as HIF-1alpha stabilization by measuring HIF-1alpha protein levels at 50 uM after 2 hrs by Western blot analysis | Homo sapiens | 21 | cell-based format | Scientific Literature | ||
| 9. | ALA4219888 | B | Binding affinity to VBC (unknown origin) assessed as association rate constant at 50 uM after 60 secs at 10 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 10. | ALA4219889 | B | Binding affinity to VBC (unknown origin) assessed as dissociation rate constant at 50 uM after 160 secs at 10 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 11. | ALA4219890 | B | Binding affinity to VBC (unknown origin) at 50 uM at 10 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 12. | ALA4219891 | B | Binding affinity to VBC (unknown origin) assessed as dissociation half life at 50 uM at 10 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 13. | ALA4219894 | B | Binding affinity to VBC (unknown origin) assessed as association rate constant at 50 uM after 60 secs at 20 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 14. | ALA4219895 | B | Binding affinity to VBC (unknown origin) assessed as dissociation rate constant at 50 uM after 160 secs at 20 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 15. | ALA4219896 | B | Binding affinity to VBC (unknown origin) at 50 uM at 20 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 16. | ALA4219897 | B | Binding affinity to VBC (unknown origin) assessed as dissociation half life at 50 uM at 20 degC by surface plasmon resonance assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
| 17. | ALA4223598 | B | Binding affinity to VBC complex (unknown origin) assessed as reduction of FAM-labelled HIF1alpha peptide binding by fluorescence polarization assay | Homo sapiens | 4 | protein complex format | Scientific Literature | ||
| 18. | ALA4223599 | B | Binding affinity to VBC complex (unknown origin) by ITC assay | Homo sapiens | 4 | protein complex format | Scientific Literature | ||
| 19. | ALA4234785 | B | Binding affinity to N-terminal His6 tagged VHL (54 to 213 residues)/ELoC (17 to 112 residues)/EloB (1 to 120 residues) (unknown origin) complex expressed in Escherichia coli BL21(DE3) assessed as dissociation constant by ITC method | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 20. | ALA4234787 | B | Binding affinity to N-terminal His6 tagged VHL (54 to 213 residues)/ELoC (17 to 112 residues)/EloB (1 to 120 residues) (unknown origin) complex expressed in Escherichia coli BL21(DE3) assessed as change in melting temperature at 1 mM by Sypro orange dye based differential scanning fluorimetric analysis | Homo sapiens | 3 | assay format | Scientific Literature |
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