# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1767526 | B | Inhibition of recombinant full length mouse PARP-2 after 10 mins using [3H]NAD+ by solution-phase assay | Mus musculus | 24 | ALA1765088 | single protein format | Scientific Literature | |
2. | ALA1767529 | B | Inhibition of recombinant full length mouse PARP-2 by broken nuclei assay | Mus musculus | 2 | ALA1765088 | single protein format | Scientific Literature | |
3. | ALA2415548 | B | Inhibition of mouse PARP-2 | Mus musculus | 12 | ALA2413011 | single protein format | Scientific Literature | |
4. | ALA3225200 | B | Inhibition of mouse recombinant PARP2 assessed as radioactivity incorporated from [adenine-2,8-3H]NAD into proteins by liquid scintillation spectrometry | Mus musculus | 3 | ALA3217609 | single protein format | Scientific Literature | |
5. | ALA3707658 | B | Biochemical Assay: The assay is based on the use of a probe of formula (IP) that binds to the NAD binding pocket and takes advantage of the significant change in the polarization signal observed upon binding of the probe to PARP-1, -2 and -3.The probe of formula (IP) was tested for its ability to bind FL PARP-1, -2 and -3 in a titration experiment. The assay performances were then evaluated (Zâ factor) as well as the displacement of the probe by its scaffold and known commercially available PARP inhibitors. In all of the experiments, the polarization signal was measured using a Saphire2 plate reader (Tecan). Data analysis was performed using Dynafit software. In particular, titration data were fitted to the following equilibria: Enzyme+probe <==> Complex Enzyme-probe, while displacement data were fitted to the following equilibria: Enzyme+probe <==> Complex Enzyme-probe, Enzyme+Compound <==> Complex Enzyme-Compound, whereby binding of probe and compound on the enzyme are mutually exclusive. | Mus musculus | 1 | ALA3639114 | single protein format | BindingDB Database | |
6. | ALA4481022 | B | Inhibition of mouse recombinant PARP2 by chemiluminescence assay | Mus musculus | 1 | ALA4480392 | single protein format | Scientific Literature | |
7. | ALA5122261 | B | Inhibition of mouse recombinant PARP2 | Mus musculus | 1 | ALA5120795 | single protein format | Scientific Literature | |
8. | ALA5253404 | B | Inhibition of mouse recombinant PARP-2 | Mus musculus | 1 | ALA5252527 | single protein format | Scientific Literature | |
9. | ALA5253420 | B | Inhibition of mouse recombinant PARP-2 incubated for 60 mins | Mus musculus | 1 | ALA5252527 | single protein format | Scientific Literature |