#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2015473	B	Inhibition of PDHK2 at 10 uM		1		single protein format	Scientific Literature
2.	ALA1116750	B	Inhibition of PDHK2 at 5 uM		1		single protein format	Scientific Literature
3.	ALA3378294	B	Inhibition of recombinant PDHK2 (unknown origin) expressed in Escherichia coli incubated for 1 hr by ELISA	Homo sapiens	1		assay format	Scientific Literature
4.	ALA3367926	B	Inhibition of human PDK2 by Off-chip mobility shift assay	Homo sapiens	1		single protein format	Scientific Literature
5.	ALA3705869	B	Inhibition Assay: The inhibitory action of PDHK activity was indirectly evaluated by performing a kinase reaction in the presence of a test compound and measuring the residual PDH activity. For expression of hPDHK2 activity, Escherichia coli strain BL21(DE3) cells (Novagen) were transformed with the pET17b vector containing modified hPDHK2 cDNA. Escherichia coli were grown to an optical density 0.6 (600 nmol/L) at 30C. Protein expression was induced by the addition of 500 umol/L isopropyl-beta-thiogalactopyranoside. Escherichia coli were cultured at 30 C. for 5 hr and harvested by centrifugation. Resuspension of the Escherichia coli paste was disrupted by a microfluidizer. FLAG-Tagged protein was separated using FLAG affinity gel (Sigma). The gel was washed with 20 mmol/L N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid-sodium hydroxide (HEPES-NaOH), 500 mmol/L sodium chloride, 1% ethylene glycol, and 0.1% Pluronic F-68 (pH 8.0), and the binding protein was eluted with 20 mmol/L HEPES.		653		cell-based format	BindingDB Database
6.	ALA3705739	B	Inhbition Assay: For expression of hPDHK2 activity, Escherichia coli strain BL21(DE3) cells (Novagen) were transformed with the pET17b vector containing modified hPDHK2 cDNA. The Escherichia coli were grown to an optical density 0.6 (600 nmol/L) at 30 C. Protein expression was induced by the addition of 500 umol/L isopropyl-beta -thiogalactopyranoside. The Escherichia coli were cultured at 30 C. for 5 hr and harvested by centrifugation. Resuspension of the Escherichia coli paste was disrupted by a microfluidizer. FLAG-Tagged protein was purified using FLAG affinity gel. The gel was washed with 20 mmol/L HEPES-NaOH, 500 mmol/L sodium chloride, 1% ethylene glycol, and 0.1% Pluronic F-68 (pH 8.0), and the binding protein was eluted with 20 mmol/L HEPES-NaOH, 100 ug/mL FLAG peptide, 500 mmol/L sodium chloride, 1% ethylene glycol, and 0.1% Pluronic F-68 (pH 8.0). The eluted fractions containing FLAG-Tagged protein were pooled, dialyzed against 20 mmol/L HEPES-NaOH, 150 mmol/L sodium chloride.		6		cell-based format	BindingDB Database
7.	ALA3998677	B	Inhibition of N-terminal His6-SUMO tagged recombinant human PDK2 using [32P]-gamma-ATP assessed as decrease in incorporation of radioactivity into E1 protein incubated for 2 mins	Homo sapiens	26		single protein format	Scientific Literature
8.	ALA3998682	B	Inhibition of N-terminal His6-SUMO tagged recombinant human PDK2 in presence of E1 protein and PDC core E2/E3BP incubated for 10 mins by titration assay	Homo sapiens	1		single protein format	Scientific Literature
9.	ALA3998685	B	Binding affinity to N-terminal His6-SUMO tagged recombinant human PDK2 by isothermal titration calorimetry	Homo sapiens	4		single protein format	Scientific Literature
10.	ALA4008864	B	Inhibition of fluorescein-labelled VER160364 binding to PDHK2 (unknown origin) after 90 mins by fluorescence polarization assay	Homo sapiens	4		single protein format	Scientific Literature
11.	ALA4008877	B	Inhibition of PDHK2 (unknown origin) assessed as decrease in phosphorylation of E1alpha subunit at serine 293 residue after 1 hr by DELFIA	Homo sapiens	2		single protein format	Scientific Literature
12.	ALA4009651	B	Inhibition of PDK2 (unknown origin) using full length His6-tagged PDHA1 as substrate after 30 mins by ELISA	Homo sapiens	4		single protein format	Scientific Literature
13.	ALA4009660	B	Inhibition of PDK2 (unknown origin) assessed as inactivation rate using full length His6-tagged PDHA1 as substrate after 30 mins by ELISA	Homo sapiens	3		single protein format	Scientific Literature
14.	ALA4009661	B	Ratio of Kinact to Ki for PDK2 (unknown origin)	Homo sapiens	3		single protein format	Scientific Literature
15.	ALA4182058	B	Inhibition of recombinant human GST-tagged PDK2 expressed in baculovirus infected Sf9 cells using PDHA1 as substrate measured after 1 hr by ELISA	Homo sapiens	3		cell-based format	Scientific Literature
16.	ALA4311229	B	Binding affinity to SUMO-fused/His-tagged PDK2 (unknown origin) expressed in Escherichia coli by isothermal calorimetric titration analysis	Homo sapiens	4		assay format	Scientific Literature
17.	ALA4314904	B	Inhibition of human PDHK2 assessed as residual activity at 10 uM by radiometric method	Homo sapiens	1		single protein format	Scientific Literature
18.	ALA4335224	B	Inhibition of human PDHK2 assessed as residual activity at 1 uM by radiometric method	Homo sapiens	2		single protein format	Scientific Literature
19.	ALA4337047	B	Inhibition of human PDHK2 assessed as residual activity at 10 uM by scintillation counting based radiometry assay relative to control	Homo sapiens	1		single protein format	Scientific Literature
20.	ALA4351999	B	Inhibition of human PDHK2 at 10 uM by radiometric method	Homo sapiens	1		single protein format	Scientific Literature