# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3630964 | Binding | Inhibition of SphK2 (unknown origin) | Homo sapiens | 3 | ALA3627716 | single protein format | Scientific Literature | |
2. | ALA3737697 | Binding | Inhibition of human recombinant SphK2 at 500 uM preincubated for 5 mins followed by substrate addition measured for 60 mins by plate reader assay | Homo sapiens | 3 | ALA3734711 | single protein format | Scientific Literature | |
3. | ALA3737698 | Binding | Inhibition of human recombinant SphK2 at 50 uM preincubated for 5 mins followed by substrate addition measured for 60 mins by plate reader assay | Homo sapiens | 3 | ALA3734711 | single protein format | Scientific Literature | |
4. | ALA3737700 | Binding | Inhibition of human recombinant SphK2 preincubated for 5 mins followed by substrate addition measured for 60 mins by plate reader assay | Homo sapiens | 4 | ALA3734711 | single protein format | Scientific Literature | |
5. | ALA3761194 | Binding | Inhibition of SPHK2 (unknown origin) at 100 nM | Homo sapiens | 1 | ALA3758169 | single protein format | Scientific Literature | |
6. | ALA3767196 | Binding | Inhibition of SK2 (unknown origin) | Homo sapiens | 5 | ALA3763012 | single protein format | Scientific Literature | |
7. | ALA3767198 | Binding | Inhibition of recombinant human SK2 assessed as production of [32P] S1P using sphingosine as substrate at 10 uM by TLC method in presence of 100 uM [gamma32P]-ATP relative to control | Homo sapiens | 36 | ALA3763012 | single protein format | Scientific Literature | |
8. | ALA3767365 | Binding | Inhibition of recombinant human SK2 assessed as production of [32P] S1P using 10 uM sphingosine as substrate by TLC method in presence of 100 uM [gamma32P]-ATP relative to control | Homo sapiens | 3 | ALA3763012 | single protein format | Scientific Literature | |
9. | ALA3767369 | Binding | Inhibition of recombinant SK2 (unknown origin) expressed in Sf9 cells assessed as [33P]S1P formation using D-erythro sphingosine as substrate and gamma[33P]ATP by liquid scintillation counting | Homo sapiens | 1 | ALA3763012 | cell-based format | Scientific Literature | |
10. | ALA3767370 | Binding | Inhibition of recombinant SK2 (unknown origin) using sphingosine as substrate and gamma[32P]ATP by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA3763012 | single protein format | Scientific Literature | |
11. | ALA3767372 | Binding | Inhibition of human SK2 using D-erythro sphingosine as substrate and gamma[33P]ATP by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA3763012 | single protein format | Scientific Literature | |
12. | ALA3811132 | Binding | Inhibition of human SphK2 expressed in baculovirus infected in Sf9 cells using D-erythro-sphingosine as substrate and [gamma-32P]ATP by scintillation counting method | Homo sapiens | 13 | ALA3808381 | cell-based format | Scientific Literature | |
13. | ALA3816944 | Binding | Inhibition of human recombinant SphK2 remaining enzyme activity at 1 uM using sphingosine as substrate incubated for 20 mins by scintillation counting analysis in presence of [gamma-32P]ATP | Homo sapiens | 30 | ALA3813677 | single protein format | Scientific Literature | |
14. | ALA3816946 | Binding | Inhibition of human recombinant SphK2 using sphingosine as substrate incubated for 20 mins by scintillation counting analysis in presence of [gamma-32P]ATP | Homo sapiens | 6 | ALA3813677 | single protein format | Scientific Literature | |
15. | ALA3816948 | Binding | Inhibition of human recombinant SphK2 after 2 hrs by HPLC assay | Homo sapiens | 1 | ALA3813677 | single protein format | Scientific Literature | |
16. | ALA3816949 | Binding | Inhibition of SphK2 (unknown origin) | Homo sapiens | 2 | ALA3813677 | single protein format | Scientific Literature | |
17. | ALA3817021 | Binding | Inhibition of human SphK2 using sphingosine as substrate incubated for 50 mins by scintillation counting analysis in presence of [gamma-33P]ATP | Homo sapiens | 1 | ALA3813677 | single protein format | Scientific Literature | |
18. | ALA3816616 | Binding | Inhibition of full length recombinant human GST-tagged SPHK2 expressed in baculovirus expression system at 1 uM relative to control | Homo sapiens | 1 | ALA3813614 | assay format | Scientific Literature | |
19. | ALA3816957 | Binding | Inhibition of His-tagged full length human recombinant SPHK2 expressed in baculovirus infected fall armyworm Sf9 cell expression system using sphingosine as substrate by ADP-quest assay | Homo sapiens | 2 | ALA3813598 | cell-based format | Scientific Literature | |
20. | ALA3816959 | Binding | Inhibition of SPHK2 (unknown origin) | Homo sapiens | 2 | ALA3813598 | single protein format | Scientific Literature |