# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA807607 | F | Rate of phosphorylation of labeled sphingosine derivative by by human SPHK-2 expressed in HEK293 cells relative to rate of D-sphingosine | 8 | cell-based format | Scientific Literature | |||
2. | ALA864560 | B | Rate of phosphorylation by recombinant human SPHK2 using | Homo sapiens | 6 | single protein format | Scientific Literature | ||
3. | ALA1821103 | B | Inhibition of N-terminal GST-fused sphingosine kinase 2 (1-618) expressed in baculovirus expression system assessed as inhibition of sphingosine phosphorylation after 1 hr by off-chip mobility shift assay | 9 | assay format | Scientific Literature | |||
4. | ALA1929797 | B | Inhibition of SphK2 in human U937 cells assessed as change in S1P level by LC/MS analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
5. | ALA1929798 | B | Inhibition of SphK2 in human U937 cells using FTY720 as substrate assessed as decrease in phosphorylated FTY720 at 10 uM after 2 hrs by LC/MS analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
6. | ALA1929799 | B | Inhibition of SphK2 in human U937 cells using FTY720 as substrate assessed as accumulation of FTY720 at 10 uM after 2 hrs by LC/MS analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
7. | ALA1929800 | B | Inhibition of SphK2 in human U937 cells assessed as decrease in Akt phosphorylation after 16 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
8. | ALA1929801 | B | Inhibition of SphK2 in human U937 cells assessed as decrease in ERK phosphorylation after 16 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
9. | ALA1929803 | B | Inhibition of SphK2 | 1 | single protein format | Scientific Literature | |||
10. | ALA902399 | B | Effect on rate of phosphorylation of human recombinant SPHK2 expressed in HEK293 cells | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
11. | ALA2015531 | B | Inhibition of SPHK2 at 10 uM | 1 | single protein format | Scientific Literature | |||
12. | ALA1962076 | B | GSK_PKIS: SPHK2 mean inhibition at 0.1 uM [Nanosyn] | 366 | single protein format | GSK Published Kinase Inhibitor Set | |||
13. | ALA1962077 | B | GSK_PKIS: SPHK2 mean inhibition at 1 uM [Nanosyn] | 366 | single protein format | GSK Published Kinase Inhibitor Set | |||
14. | ALA2161989 | B | Inhibition of recombinant SphK2 expressed in baculovirus infected Sf9 cells using D-erythro-sphingosine as substrate and [32gammaP]ATP assessed as residual activity at 10 uM by scintillation counting | 17 | cell-based format | Scientific Literature | |||
15. | ALA2161991 | B | Inhibition of recombinant SphK2 expressed in baculovirus infected Sf9 cells using D-erythro-sphingosine as substrate and [32gammaP]ATP by scintillation counting | 7 | cell-based format | Scientific Literature | |||
16. | ALA2161992 | B | Inhibition of human recombinant SphK2 using NBD-sphingosine as substrate after 2 hrs by HPLC analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
17. | ALA2161993 | B | Inhibition of SphK2 using sphingosine as substrate and [32gammaP]ATP after 15 to 20 mins by Cerenkov counting analysis | 1 | single protein format | Scientific Literature | |||
18. | ALA2157705 | B | Inhibition of SPHK2 at 1 uM | 1 | single protein format | Scientific Literature | |||
19. | ALA995443 | B | Inhibition of human cloned SPHK2 expressed in CHO cells at 10 uM | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
20. | ALA995445 | B | Inhibition of human cloned SPHK2 expressed in CHO cells using D-erythro-sphingosine as substrate by Michaelis-Menten plot | Homo sapiens | 1 | cell-based format | Scientific Literature |