#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3429874	Binding	Inhibition of human C-RAF	Homo sapiens	1	ALA3425429	single protein format	Scientific Literature
2.	ALA3428987	Binding	Inhibition of human RAF1 using MEK1 (K97R) peptide substrate at 1 uM by 33P Hotspot assay	Homo sapiens	1	ALA3425491	single protein format	Scientific Literature
3.	ALA3578337	Binding	Inhibition of wild type CRAF (unknown origin) assessed as ADP formation measured for 5 hrs by pyruvate kinase/lactate dehydrogenase coupled assay in presence of ATP, MEK1, PEP, NADH	Homo sapiens	11	ALA3576847	single protein format	Scientific Literature
4.	ALA3578908	Binding	Competitive binding affinity to CRAF in human A375 cells after 15 mins in presence of ATP analogue	Homo sapiens	3	ALA3576847	cell-based format	Scientific Literature
5.	ALA3595101	Binding	Inhibition of RAF1 (unknown origin)	Homo sapiens	1	ALA3593212	single protein format	Scientific Literature
6.	ALA3595420	Binding	Inhibition of human RAF1 Y340D/Y341D double mutant at 0.1 uM relative to control	Homo sapiens	1	ALA3593229	single protein format	Scientific Literature
7.	ALA3616265	Binding	Inhibition of human c-RAF at 1 uM	Homo sapiens	1	ALA3611990	single protein format	Scientific Literature
8.	ALA3620011	Binding	Inhibition of RAF1 (unknown origin) at 10 uM after 40 mins by scintillation counting analysis	Homo sapiens	2	ALA3616395	single protein format	Scientific Literature
9.	ALA3620321	Binding	Inhibition of C-Raf (unknown origin) after 40 mins by scintillation counting analysis	Homo sapiens	1	ALA3616395	single protein format	Scientific Literature
10.	ALA3620367	Binding	Inhibition of wild type C-RAF (unknown origin)	Homo sapiens	2	ALA3616393	single protein format	Scientific Literature
11.	ALA3625833	Binding	Inhibition of human recombinant c-RAF	Homo sapiens	1	ALA3621115	single protein format	Scientific Literature
12.	ALA3631110	Binding	Inhibition of RAF1 (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
13.	ALA3630463	Binding	Inhibition of human RAF1 at 10 uM by kinase inhibition assay	Homo sapiens	3	ALA3627617	single protein format	Scientific Literature
14.	ALA3630473	Binding	Inhibition of human RAF1 by kinase inhibition assay	Homo sapiens	1	ALA3627617	single protein format	Scientific Literature
15.	ALA3631058	Binding	Inhibition of Raf (unknown origin) by radiometric protein kinase assay	Homo sapiens	8	ALA3627661	single protein format	Scientific Literature
16.	ALA3631577	Binding	Inhibition of RAF1 (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control	Homo sapiens	1	ALA3627674	single protein format	Scientific Literature
17.	ALA3636909	Binding	Inhibition of wild type c-RAF (unknown origin)	Homo sapiens	1	ALA3632535	single protein format	Scientific Literature
18.	ALA3705982	Binding	ADP-Glo Kinase Assay: Inhibitor Concentration at 50% enzyme inhibition (IC50) values were calculated by quantifying the end-point ADP production from each kinase reaction using the ADP-Glo Kinase Assay (Promega, Madison, Wis.) as described by the manufacturer. Reactions were performed in Tris buffer (50 mM pH 7.5, RT) containing 20 mM MgCl2 and 0.1% Bovine Serum Albumin. Each assay was performed in 60 uL of the solution in 10x75 mm borosilicate glass test tubes and allowed to continuously shake during the duration of the assay. The total ADP generated was quantified by transferring 25 uL (2x) of each assay to a 96-well luminescence assay plate, followed by the addition of 25 uL of ADP-Glo Reagent (45 min incubation) to remove any remaining ATP. For luminescence readings, 50 uL of Kinase Detection Reagent (45 min incubation) was added to convert the ADP generated from the kinase reaction to ATP, and luminescent intensity was measured using a luciferase/luciferin reaction.	Homo sapiens	17	ALA3638864	single protein format	BindingDB Database
19.	ALA3707927	Binding	Kinase Assay: Inhibition of kinase activity by test compound disclosed herein was estimated by quantifying the amount of [33P] incorporation of substrate in the presence of test compound. Standard assay conditions were 2 ng of recombinant C-Raf or 5 ng of recombinant B-Raf or 5 ng of recombinant B-Raf (V600E) kinase (Upstate Biotechnology) with 500 ng MEK1(K97R) in assay buffer {8 μM ATP, 0.5 μCi [33P]ATP (specific activity 3000 Ci/mmol, PerkinElmer), 50 mM Tris/HCl (pH7.5), and 1 mM EGTA, 1 mM Na3VO4, 1% 2-mercaptoethanol, 0.1% Brij 35, and 0.2 mg/ml BSA} at a final volume of 25 μL. Reactions were incubated at 30° C. for 30 min and stopped by adding 3% phosphoric acid, harvested onto a 96-well GF/B UniFilter (PerkinElmer) using a unifilter harvester (PerkinElmer), and counted with a TopCount microplate scintillation counter (PerkinElmer). The IC50 values of inhibitors were determined after carrying out assays at 3-fold serially diluted concentrations of each compound in duplication.	Homo sapiens	127	ALA3638563	single protein format	BindingDB Database
20.	ALA3721233	Binding	Inhibition of human C-raf using biotin-MEKl substrate incubated for 1 hr	Homo sapiens	5	ALA3714760	single protein format	Patent Bioactivity Data