#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3428402	Binding	Inhibition of SYK (unknown origin) assessed as remaining activity at 100 uM by radioactive filter binding assay in presence of [33P]-ATP relative to control	Homo sapiens	16	ALA3425453	single protein format	Scientific Literature
2.	ALA3583143	Binding	Inhibition of Tel-fused SYK (unknown origin) expressed in mouse BA/F3 cells after 48 hrs by luciferase reporter gene assay in absence of recombinant mouse IL3	Homo sapiens	3	ALA3580662	cell-based format	Scientific Literature
3.	ALA3595082	Binding	Inhibition of SYK (unknown origin)	Homo sapiens	1	ALA3593212	single protein format	Scientific Literature
4.	ALA3606876	Binding	Inhibition of SYK (unknown origin) at 2 uM by radioactive filter-binding assay	Homo sapiens	1	ALA3603808	single protein format	Scientific Literature
5.	ALA3606895	Binding	Inhibition of SYK (unknown origin) at 250 nM by radioactive filter-binding assay	Homo sapiens	1	ALA3603808	single protein format	Scientific Literature
6.	ALA3606461	Binding	Inhibition of human SYK	Homo sapiens	1	ALA3603755	single protein format	Scientific Literature
7.	ALA3620014	Binding	Inhibition of SYK (unknown origin) at 10 uM after 40 mins by scintillation counting analysis	Homo sapiens	2	ALA3616395	single protein format	Scientific Literature
8.	ALA3620307	Binding	Inhibition of SYK (unknown origin) after 40 mins by scintillation counting analysis	Homo sapiens	1	ALA3616395	single protein format	Scientific Literature
9.	ALA3624367	Binding	Inhibition of human recombinant Syk	Homo sapiens	1	ALA3621115	single protein format	Scientific Literature
10.	ALA3627015	Binding	Inhibition of purified full-length human SYK pre-incubated for 30 mins at room temperature before Ulight-TK peptide substrate addition and measured 1 hr after substrate addition by cell free kinase assay	Homo sapiens	40	ALA3621069	single protein format	Scientific Literature
11.	ALA3627019	Binding	Inhibition of SYK-mediated TNFalpha production in human IgG-stimulated human THP1 cells incubated for 18 hrs by ELISA method	Homo sapiens	4	ALA3621069	cell-based format	Scientific Literature
12.	ALA3627026	Binding	Inhibition of purified full-length human SYK at 0.1 uM pre-incubated for 30 mins at room temperature before Ulight-TK peptide substrate addition and measured 1 hr after substrate addition by cell free kinase assay	Homo sapiens	1	ALA3621069	single protein format	Scientific Literature
13.	ALA3627027	Binding	Inhibition of purified full-length human SYK at 0.01 uM pre-incubated for 30 mins at room temperature before Ulight-TK peptide substrate addition and measured 1 hr after substrate addition by cell free kinase assay	Homo sapiens	1	ALA3621069	single protein format	Scientific Literature
14.	ALA3627444	Binding	Inhibition of Syk (unknown origin) using 5-Fluo-Ahx-GAPDYENLQELNKK-Amide as substrate after 60 mins by microfluidic mobility shift assay	Homo sapiens	13	ALA3621151	single protein format	Scientific Literature
15.	ALA3627445	Binding	Inhibition of Syk in anti-igM stimulated human Ramos B cells assessed as phospho-BLNK level preincubated for 30 mins followed by anti-IgM stimulation measured after 15 mins by flow cytometric analysis	Homo sapiens	13	ALA3621151	cell-based format	Scientific Literature
16.	ALA3627446	Binding	Inhibition of Syk in anti-CD32 stimulated CD14+ human monocytes in presence of 90% human blood assessed as phospho-SLP76 level preincubated for 30 mins followed by anti-CD32 stimulation measured after 5 mins by flow cytometric analysis	Homo sapiens	13	ALA3621151	assay format	Scientific Literature
17.	ALA3631488	Binding	Inhibition of SYK (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
18.	ALA3631695	Binding	Inhibition of Syk (unknown origin) at 1 uM by FRET method	Homo sapiens	1	ALA3627690	single protein format	Scientific Literature
19.	ALA3705225	Binding	Homogeneous Time-Resolved Fluorescence (HTRF) Assay : A recombinant GST-hSYK fusion protein was used to measure potency of compounds to inhibit human SYK activity. The recombinant human GST-SYK (Carna Biosciences #08-176) (5 pM final concentration) was incubated with various concentrations of the inhibitor diluted in DMSO (0.1% final concentration) for 10 minutes at room temperature in 15 mM Tris-HCl (pH 7.5), 0.01% tween 20, 2 mM DTT in 384 well plate format. To initiate the reaction the biotinylated substrate peptide (250 nM final concentration) that contains the phosphorylation site for SYK was added with magnesium (5 mM final concentration) and ATP (25 μM final concentration). Final volume of the reaction was 10 μL. Phosphorylation of the peptide was allowed to proceed for 45′ at room temperature.	Homo sapiens	46	ALA3639355	single protein format	BindingDB Database
20.	ALA3705362	Binding	Mobility-Shift kinase assay: Compounds of the examples provided herein were assessed for their ability to inhibit Syk kinase by utilizing Caliper Life Sciences' proprietary LabChip technology. The off-chip incubation mobility-shift kinase assay uses a microfluidic chip to measure the conversion of a fluorescent peptide substrate to a phosphorylated product. The reaction mixture, from a microtiter plate well, is introduced through a capillary sipper onto the chip, where the nonphosphorylated substrate and phosphorylated product are separated by electrophoresis and detected via laser induced fluorescence. The signature of the fluorescence signal over time reveals the extent of the reaction. The phosphorylated product migrates through the chip faster than the non-phosphorylated substrate, and signals from the two forms of the peptide appear as distinct peaks.	Homo sapiens	722	ALA3639055	single protein format	BindingDB Database