#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3428239	Binding	Inhibition of PLK1 (unknown origin) assessed as remaining activity at 100 uM by radioactive filter binding assay in presence of [33P]-ATP relative to control	Homo sapiens	16	ALA3425453	single protein format	Scientific Literature
2.	ALA3430830	Binding	Delta TM value showing the stabilisation of PLK1 produced by compound binding	Homo sapiens	156	ALA1145498	single protein format	Scientific Literature
3.	ALA3584678	Binding	Inhibition of PLK1 Thr210 phosphorylation in human HeLa cells at 100 nM by immunoblotting method	Homo sapiens	1	ALA3580605	cell-based format	Scientific Literature
4.	ALA3584679	Binding	Inhibition of PLK1 Thr210 phosphorylation in human DU145 cells at 100 nM by immunoblotting method	Homo sapiens	1	ALA3580605	cell-based format	Scientific Literature
5.	ALA3584682	Binding	Inhibition of PLK1 Thr210 phosphorylation in human HeLa cells at 10 uM by immunoblotting method	Homo sapiens	4	ALA3580605	cell-based format	Scientific Literature
6.	ALA3584683	Binding	Inhibition of PLK1 Thr210 phosphorylation in human DU145 cells at 10 uM by immunoblotting method	Homo sapiens	4	ALA3580605	cell-based format	Scientific Literature
7.	ALA3595485	Binding	Inhibition of human PLK1 at 0.1 uM relative to control	Homo sapiens	1	ALA3593229	single protein format	Scientific Literature
8.	ALA3607885	Binding	Binding affinity to DNA-tagged PLK (unknown origin) at 10 uM by qPCR analysis	Homo sapiens	2	ALA3603810	single protein format	Scientific Literature
9.	ALA3606458	Binding	Inhibition of human PLK1	Homo sapiens	1	ALA3603755	single protein format	Scientific Literature
10.	ALA3610437	Binding	Binding affinity to PLK1 (unknown origin) expressed in HEK293 cells after 1 hr by proprietary competition assay	Homo sapiens	18	ALA3608255	cell-based format	Scientific Literature
11.	ALA3620010	Binding	Inhibition of PLK1 (unknown origin) at 10 uM after 40 mins by scintillation counting analysis	Homo sapiens	2	ALA3616395	single protein format	Scientific Literature
12.	ALA3620303	Binding	Inhibition of PLK1 (unknown origin) after 40 mins by scintillation counting analysis	Homo sapiens	1	ALA3616395	single protein format	Scientific Literature
13.	ALA3624365	Binding	Inhibition of human recombinant Plk1	Homo sapiens	1	ALA3621115	single protein format	Scientific Literature
14.	ALA3630945	Binding	Inhibition of PLK1 (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
15.	ALA3631689	Binding	Inhibition of PLK1 (unknown origin) at 1 uM by FRET method	Homo sapiens	1	ALA3627690	single protein format	Scientific Literature
16.	ALA3631573	Binding	Inhibition of PLK1 (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control	Homo sapiens	1	ALA3627674	single protein format	Scientific Literature
17.	ALA3705553	Binding	Biochemical Assay: The inhibitory activity of putative kinase inhibitors and the potency of selected compounds were determined using a trans-phosphorylation assay.	Homo sapiens	2	ALA3638564	single protein format	BindingDB Database
18.	ALA3705783	Binding	Biochemical Assay: The inhibitory activity of putative kinase inhibitors and the potency of selected compounds were determined using a trans-phosphorylation assay.Specific peptide or protein substrates are trans-phosphorylated by their specific serine-threonine or tyrosine kinase, in the presence of ATP traced with 33P-gamma-ATP, and in the presence of their own optimal buffer and cofactors.At the end of the phosphorylation reaction, more than 98% cold ATP and radioactive ATP is captured by an excess of the ion exchange dowex resin; the resin then settles down to the bottom of the reaction plate by gravity.Supernatant, containing the phosphorylated substrate, is subsequently withdrawn and transferred into a counting plate, then evaluated by beta-counting.Reagents/Assay Conditions i. Dowex Resin Preparation500 g of wet resin (SIGMA, custom prepared resin DOWEX 1x8 200-400 mesh, 2.5 Kg) are weighed out and diluted to 2 L in 150 mM sodium formate, pH 3.00.The resin is allowed to settle down.	Homo sapiens	15	ALA3638516	single protein format	BindingDB Database
19.	ALA3707850	Binding	Kinase Inhibition Assay: The compound of Example 39, 2-(1H-indol-5-ylamino)-6-(2,4-difluorophenylsulfonyl)-8-methylpyrido[2,3-d]pyrimidin-7(8H)-one, was subjected to a kinase inhibition assay for the kinases. Compounds were tested in 5 dose IC50 mode with 10-fold serial dilution starting at 10 μM. Staurosporine, a known protein kinase inhibitor, was tested in 5-dose IC50 mode with 3-fold serial dilution starting at 20 μM. Reactions were carried out in 10 μM ATP. The results are shown in Table 5. The results show that the compound of Example 39 is a kinase inhibitor highly selective for the kinase Plk2.	Homo sapiens	2	ALA3639034	single protein format	BindingDB Database
20.	ALA3742678	Binding	Inhibition of PLK1 (unknown origin)	Homo sapiens	1	ALA3739276	single protein format	Scientific Literature