Activity at Streptomyces antibioticus wild-type OleD assessed as as rate of glucoside formation measured as nanomoles of product formed per minute per mg of enzyme
Activity of Streptomyces antibioticus OleD P67T mutant assessed as as rate of glucoside formation measured as nanomoles of product formed per minute per mg of enzyme
Activity of Streptomyces antibioticus OleD S123F mutant assessed as as rate of glucoside formation measured as nanomoles of product formed per minute per mg of enzyme
Activity of Streptomyces antibioticus OleD A242V mutant assessed as as rate of glucoside formation measured as nanomoles of product formed per minute per mg of enzyme
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as as rate of glucoside formation measured as nanomoles of product formed per minute per mg of enzyme
Ratio of Kcat to Km for Streptomyces antibioticus wild-type OleD assessed as as rate of glucoside formation using varying compound concentration by enzyme kinetics assay
Activity of Streptomyces antibioticus wild-type OleD assessed as as rate of glucoside formation at 10 mM using varying NDP sugar UDP-glucose concentration by enzyme kinetics assay
Ratio of Kcat to Km for Streptomyces antibioticus wild-type OleD assessed as as rate of glucoside formation at 10 mM using varying NDP sugar UDP-glucose concentration by enzyme kinetics assay
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as as rate of glucoside formation using varying compound concentration by enzyme kinetics assay
Ratio of Kcat to Km for Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as as rate of glucoside formation using varying compound concentration by enzyme kinetics assay
Activity of Streptomyces antibioticus wild-type OleD assessed as conversion rate of compound to produce glucoside 3 using NDP sugar UDP-glucose after 3 hrs by HPLC
Activity of Streptomyces antibioticus wild-type OleD assessed as conversion rate of compound to produce glucoside 3 using NDP sugar UDP-6-deoxy-glucose after 3 hrs by HPLC
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as rate of glucoside formation at 10 mM using varying NDP sugar UDP-glucose concentration by enzyme kinetics assay
Ratio of Kcat to Km for Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as rate of glucoside formation at 10 mM using varying NDP sugar UDP-glucose concentration by enzyme kinetics assay
Activity of Streptomyces antibioticus wild-type OleD assessed as conversion rate of compound to produce glucoside 3 using NDP sugar donor UDP-6-thio-glucose after 3 hrs by HPLC
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as conversion rate of compound to produce glucoside 3 using NDP sugar UDP-glucose after 3 hrs by HPLC
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as conversion rate of compound to produce glucoside 3 using NDP sugar UDP-xylose after 3 hrs by HPLC
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as conversion rate of compound to produce glucoside 3 using NDP sugar UDP-6-deoxy-glucose after 3 hrs by HPLC
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as conversion rate of compound to produce glucoside 3 using NDP sugar donor UDP-6-thio-glucose after 3 hrs by HPLC
Activity of Streptomyces antibioticus OleD P67T/S132F/A242V mutant assessed as conversion rate of compound to produce glucoside 3 using NDP sugar UDP-6-azido-glucose after 3 hrs by HPLC