| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3778806 | Binding | Inhibition of recombinant human N-terminal GST-tagged KDM4B (1 to 500 residues) expressed in baculovirus infected sf9 cells using biotin-H3K9me3 as substrate preincubated for 10 mins measured after 20 mins by alphascreen assay | Homo sapiens | 56 | ALA3774338 | cell-based format | Scientific Literature | |
| 2. | ALA3776210 | Binding | Inhibition of recombinant human N-terminal GST-tagged KDM4B (1 to 500 residues) expressed in baculovirus infected sf9 cells using biotin-H3K9me3 as substrate at 100 uM preincubated for 10 mins measured after 20 mins by alphascreen assay relative to control | Homo sapiens | 5 | ALA3774338 | cell-based format | Scientific Literature | |
| 3. | ALA3791427 | Binding | Inhibition of human N-terminal GST-tagged KDM4B (1 to 500 residues) expressed in baculovirus infected sf9 cells using H3K4me3 as substrate | Homo sapiens | 3 | ALA3784917 | cell-based format | Scientific Literature | |
| 4. | ALA3789210 | Binding | Inhibition of human N-terminal GST-tagged KDM4B (1 to 500 residues) expressed in baculovirus infected sf9 cells using biotinylated histone H3 as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by AlphaLisa assay | Homo sapiens | 3 | ALA3784917 | cell-based format | Scientific Literature | |
| 5. | ALA4353689 | Binding | Inhibition of N-terminal GST-tagged recombinant human KDM4B (1 to 500 residues) expressed in baculovirus infected Sf9 insect cells assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay | Homo sapiens | 33 | ALA4350996 | cell-based format | Scientific Literature | |
| 6. | ALA4353708 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 0.25 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 7. | ALA4353709 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 0.5 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 8. | ALA4353710 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 1 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 9. | ALA4353711 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 10. | ALA4353712 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 4 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 11. | ALA4353713 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 8 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 12. | ALA4353714 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 16 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 13. | ALA4353715 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 50 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 14. | ALA4353716 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 100 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 15. | ALA4353717 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 300 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 16. | ALA4353718 | Binding | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 1000 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 17. | ALA4353733 | Binding | Binding affinity to recombinant human N-terminal KDM4B (1 to 348 residues) fused upstream with E31G-R71G-K105E/ePL-tagged C-terminal transfected in HEK293T cells measured after 30 mins by chemiluminescence based cellular target engagement assay | Homo sapiens | 8 | ALA4350996 | cell-based format | Scientific Literature | |
| 18. | ALA4353747 | Binding | Ratio of IC50 for inhibition of full length human KDM4B in presence of 1000 uM 2OG as co-substrate to IC50 for inhibition of full length human KDM4B in presence of 0.25 uM 2OG as cosubstrate | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 19. | ALA3363695 | Binding | Inhibition of recombinant human KDM4B (1 to 348 aa) expressed in Escherichia coli using calf thymus histone H3 as substrate after 30 mins by Western blotting | Homo sapiens | 1 | ALA3352546 | assay format | Scientific Literature | |
| 20. | ALA3363697 | Binding | Inhibition of recombinant human KDM4B (1 to 348 aa) expressed in Escherichia coli using H3K9me3 peptide as substrate assessed as residual activity after 30 mins by FDH-coupled assay | Homo sapiens | 1 | ALA3352546 | assay format | Scientific Literature |
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