# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5141061 | B | Inhibition of human recombinant His-tagged MAT2A expressed in Escherichia coli expression system assessed as S-adenosyl methionine production using L-methionine as substrate incubated for 20 mins followed by L-methionine and ATP addition by malachite green phosphate assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
2. | ALA5141062 | B | Inhibition of N-terminal TEV cleavable 6His-tagged human MAT2A (1 to 395 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as S-adenosyl methionine production using L-methionine as substrate incubated for 30 mins followed by L-methionine and ATP addition by MS/MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
3. | ALA5141063 | B | Inhibition of MAT2A in human NCI-H520 cells transfected with MAT2A empty vector assessed as inhibition of S-adenosyl methionine production measured after 6 hrs by Rapid Fire Mass Spectrometry analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA5141064 | B | Inhibition of MAT2A in human Huh-7 cells assessed as inhibition of S-adenosyl methionine production measured after 6 hrs by Rapid Fire Mass Spectrometry analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
5. | ALA5141066 | B | Inhibition of human MAT2A expressed in baculovirus infected Sf9 cells expression system assessed as S-adenosyl methionine production using L-methionine as substrate pretreated for 60 mins followed by L-methionine and ATP addition | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
6. | ALA5141069 | B | Inhibition of MAT2A (unknown origin) assessed as S-adenosyl methionine production using L-methionine as substrate pretreated for 60 mins followed by L-methionine and ATP addition | Homo sapiens | 4 | single protein format | Scientific Literature | ||
7. | ALA5141072 | B | Inhibition of human MAT2A assessed as S-adenosyl methionine production using L-methionine as substrate pretreated for 60 mins followed by L-methionine and ATP addition by colorimetric analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
8. | ALA3378077 | B | Inhibition of recombinant His-tagged MAT2A (unknown origin) using L-methionine and ATP at 30 uM | Homo sapiens | 15 | single protein format | Scientific Literature | ||
9. | ALA3991784 | B | Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry. | Homo sapiens | 220 | subcellular format | Kuster lab chemical proteomics drug profiling | ||
10. | ALA4263252 | B | Binding affinity to GST-tagged MAT2A (unknown origin) expressed in Escherichia coli BL21 by Western blot based pull down assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
11. | ALA4476981 | B | Inhibition of recombinant His6-tagged MAT2A (unknown origin) expressed in SF9 insect cells using L-Met as substrate preincubated for 60 mins followed by substrate and ATP addition and measured after 60 mins by spectrophotometric analysis | Homo sapiens | 246 | cell-based format | Patent Bioactivity Data | ||
12. | ALA4723090 | B | Allosteric inhibition of MAT2A (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as reduction in S-adenosyl-methionine formation using methionine as substrate in presence of ATP by ELISA | Homo sapiens | 1 | assay format | Scientific Literature | ||
13. | ALA4725015 | B | Inhibition of recombinant human MAT2A expressed in baculovirus infected Sf9 cells using L-methionine as substrate incubated for 60 mins followed by substrate addition and measured after 60 mins by S-adenosyl methionine assay kit method | Homo sapiens | 10 | cell-based format | Scientific Literature | ||
14. | ALA4725016 | B | Inhibition of MAT2A in human HCT-116 cells assessed as reduction in SAM incubated for 72 hrs by LC-MS/MS analysis | Homo sapiens | 10 | cell-based format | Scientific Literature | ||
15. | ALA4835535 | B | Binding affinity to MAT2A (unknown origin) by surface plasmon resonance assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
16. | ALA4835538 | B | Inhibition of MAT2A (unknown origin) mass spectrometry-based ultrafiltration assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
17. | ALA4835540 | B | Inhibition of recombinant human MAT2A expressed in baculovirus infected Sf9 cells assessed as S-adenosyl methionine production using L-methionine as substrate incubated for 60 mins followed by substrate addition and measured after 60 mins | Homo sapiens | 46 | cell-based format | Scientific Literature | ||
18. | ALA4835541 | B | Inhibition of recombinant human MAT2A expressed in baculovirus infected Sf9 cells assessed as S-adenosyl methionine production using L-methionine as substrate incubated for 60 mins followed by substrate addition and measured after 60 mins relative to control | Homo sapiens | 45 | cell-based format | Scientific Literature | ||
19. | ALA5036614 | B | Binding affinity to full length recombinant human N-terminal His6-tagged MAT2A expressed in Escherichia coli assessed as dissociation constant by SPR analysis | Homo sapiens | 4 | assay format | Scientific Literature | ||
20. | ALA5036615 | B | Inhibition of full length recombinant human N-terminal His6-tagged MAT2A expressed in Escherichia coli using methionine and ATP as substrate incubated for 90 mins by biomol green reagent based assay | Homo sapiens | 33 | assay format | Scientific Literature |