#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA707395	B	Compound was tested to inhibit hepatic lipase (HL)		5	ALA1148382	single protein format	Scientific Literature
2.	ALA900761	B	Inhibition of hepatic lipase		2	ALA1137574	single protein format	Scientific Literature
3.	ALA1015041	B	Inhibition of hepatic lipase activity assessed as release of free fatty acid from hydrolysis of VLDL by cell based assay		2	ALA1152778	cell-based format	Scientific Literature
4.	ALA2384496	B	Inhibition of human HL expressed in HEK293 cells using PED-A1 as substrate by spectrophotometry	Homo sapiens	1	ALA2380223	cell-based format	Scientific Literature
5.	ALA3095191	B	Inhibition of recombinant human hepatic lipase using bis-BODIPY-FL C11-PC as substrate preincubated for 30 mins followed by substrate addition by FELA method	Homo sapiens	21	ALA3091416	single protein format	Scientific Literature
6.	ALA3707962	B	Biological Assay: Endothelial lipase (EL) and hepatic lipase (HL) activities were measured using a fluorescent substrate, A10070, (Invitrogen, CA) doped into an artificial vesicle containing DMPG (Avanti Polar Lipids) as the excipient. Vesicles were prepared by combining 571 uL of 29 mM DMPG in a 1:1 mixture of MeOH and CHCl3 with 2000 uL of 1 mM A10070 in a 1:1 mixture of MeOH and CHCl3. The mixture was dried under nitrogen in multiple vials then resuspended in 20 mL total volume of 50 mM HEPES pH 8.0 buffer containing 50 mM NaCl and 0.2 mM EDTA. The sample was allowed to sit at room temperature for 15 min and then was sonicated 3x4 mins on ice with a Branson Sonicator using duty cycle 1. This preparation provides vesicles with a mole fraction of 0.11 for the FRET substrate.The enzymatic assay was measured using 384-well white Optiplates. Each well contained 20 uL of assay buffer (50 mM HEPES pH 8.0, 50 mM NaCl and 1 mM CaCl2) and 0.25 uL of a DMSO solution containing a compound.		340	ALA3638990	single protein format	BindingDB Database
7.	ALA4199685	B	Inhibition of recombinant human HL expressed in African green monkey COS7 cells using HDL as substrate pretreated for 10 mins followed by substrate addition and measured after 30 mins by LC/MS/MS analysis	Homo sapiens	2	ALA4196117	cell-based format	Scientific Literature
8.	ALA4325753	B	Inhibition of human HL expressed in human COS7 cells using A10070 as substrate preincubated for 20 min followed by DMPG vesicle doped substrate addition and measured at 20 secs interval for 10 mins by fluorescence assay	Homo sapiens	16	ALA4321883	cell-based format	Scientific Literature
9.	ALA4385276	B	Inhibition of recombinant human HL expressed in COS7 cells using PED-A1 containing DMPG vesicles as substrate pretreated for 20 mins followed by substrate addition and measured every 20 secs for 10 mins by fluorescence assay	Homo sapiens	15	ALA4382275	cell-based format	Scientific Literature
10.	ALA4390140	B	Inhibition of human hepatic lipase expressed in African green monkey COS7 cells using PED-A1 as substrate incubated for 20 mins and measured every 20 secs for 10 mins by FRET assay	Homo sapiens	11	ALA4387752	cell-based format	Scientific Literature
11.	ALA4400383	A	Inhibition of hepatic lipase (unknown origin)	Homo sapiens	14	ALA4396970	single protein format	Scientific Literature
12.	ALA5305019	B	Hepatic lipase Endocannabinoid related and lipase panel	Homo sapiens	1	ALA5303769	single protein format	Donated Chemical Probes - SGC Frankfurt