#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3396798	B	Induction of human ferroportin degradation expressed in HEK293 cells after 24 hrs by flow cytometry	Homo sapiens	9		cell-based format	Scientific Literature
2.	ALA3624279	B	Agonist activity at GFP-fused human FPN transfected in HEK293 cells assessed as protein degradation after 24 hrs by flow cytometric analysis	Homo sapiens	30		cell-based format	Scientific Literature
3.	ALA3883076	B	Antagonist activity at C-terminal halo-tag fused human ferroportin expressed in MDCK cells assessed as inhibition of hepcidin-induced ferroportin internalization after overnight incubation by Draq5 staining-based fluorescence assay	Homo sapiens	250		cell-based format	Patent Bioactivity Data
4.	ALA4419455	B	Binding affinity to C-terminal FLAG-tagged human ferroportin expressed in Pichia pastoris assessed as reduction in inhibition of binding of TMR-hepcidin to Fpn incubated for 90 mins by fluorescence polarization assay	Homo sapiens	185		assay format	Patent Bioactivity Data
5.	ALA4419456	B	Inhibition of GFP-tagged human ferroportin expressed in HEK293 #354 cells assessed as reduction in Fpn-mediated iron efflux incubated overnight by iron regulatory element -driven beta-lactamase reporter gene assay	Homo sapiens	130		cell-based format	Patent Bioactivity Data
6.	ALA4419457	B	Inhibition of GFP-tagged human ferroportin expressed in HEK293 #354 cells assessed as induction of ferroportin internalization and degradation incubated overnight by FACS assay	Homo sapiens	107		cell-based format	Patent Bioactivity Data
7.	ALA4419478	B	Inhibition of ferroportin in human T47D cells assessed as blocking of iron export incubated for 20 hrs using [58]Fe by inductively coupled plasma mass spectrometry	Homo sapiens	1		cell-based format	Patent Bioactivity Data
8.	ALA4431470	B	Inhibition of recombinant human C-terminal nano luciferase-tagged SLC40A1 expressed in human T-REx-293 cells assessed as decrease in 55Fe-apotransferrin export at 10 uM preincubated for 24 hrs followed by export buffer and compound addition measured after 5 mins by scintillation counting method	Homo sapiens	1		cell-based format	Scientific Literature
9.	ALA4431471	B	Inhibition of recombinant human SLC40A1 expressed in human T-REx-293 cells assessed as reduction in ferroportin internalization incubated for 20 hrs by luciferase reporter gene assay	Homo sapiens	3		cell-based format	Scientific Literature
10.	ALA4431472	B	Inhibition of recombinant human SLC40A1 expressed in human T-REx-293 cells exposed to 4 hrs of doxycycline induction assessed as reduction in ferroportin internalization incubated for 20 hrs by luciferase reporter gene assay relative to control	Homo sapiens	1		cell-based format	Scientific Literature
11.	ALA4431473	B	Inhibition of recombinant human SLC40A1 expressed in human T-REx-293 cells exposed to >4 hrs of doxycycline induction assessed as reduction in ferroportin internalization incubated for 20 hrs by luciferase reporter gene assay relative to control	Homo sapiens	1		cell-based format	Scientific Literature
12.	ALA4431474	B	Inhibition of recombinant human C-terminal nano luciferase-tagged SLC40A1 expressed in human T-REx-293 cells assessed as ferroportin internalization incubated for 20 hrs measured at 459 nanometer by Hoechst staining based cellomics arrayscan imaging analysis	Homo sapiens	1		cell-based format	Scientific Literature
13.	ALA4431475	B	Binding affinity to recombinant human C-terminal nano luciferase-tagged SLC40A1 expressed in human T-REx-293 cells assessed as internalization of compound-ferroportin conjugate at 0.0001 to 0.01 mg/ml incubated for 20 hrs measured at 549 nanometer by Hoechst staining based cellomics arrayscan imaging analysis	Homo sapiens	1		cell-based format	Scientific Literature
14.	ALA4431476	B	Inhibition of human GFP-tagged SLC40A1 expressed in HEK293 cells assessed as ferroportin internalization by fluorescence microscopic analysis	Homo sapiens	1		cell-based format	Scientific Literature