# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3608054 | Binding | Inhibition of human 15-LOX-2 expressed in Sf9 cells assessed as reduction in conversion of arachidonic acid to 15-HETE pre-incubated 5 min before arachidonic acid substrate addition by HPLC-UV spectroscopy | Homo sapiens | 3 | ALA3603736 | cell-based format | Scientific Literature | |
2. | ALA3766394 | Binding | Inhibition of N-terminal His6-tagged human 15-LOX-2 using arachidonic acid as substrate by UV-visible spectrophotometric analysis | Homo sapiens | 1 | ALA3763110 | single protein format | Scientific Literature | |
3. | ALA3777207 | Binding | Binding affinity to recombinant human 15-Lipoxygenase-2 at 10 uM | Homo sapiens | 1 | ALA3774360 | single protein format | Scientific Literature | |
4. | ALA897957 | Binding | Inhibition of human recombinant 15-lipoxygenase over-expressed in CHO cells | Homo sapiens | 5 | ALA1149679 | cell-based format | Scientific Literature | |
5. | ALA897958 | Binding | Inhibition of human recombinant 15-lipoxygenase over-expressed in CHO cells at 10 uM | Homo sapiens | 5 | ALA1149679 | cell-based format | Scientific Literature | |
6. | ALA924097 | Binding | Inhibition of 15-hLO2 | 51 | ALA1140486 | single protein format | Scientific Literature | ||
7. | ALA1273493 | Binding | Inhibition of human N-terminal His6-tagged epithelial 15-lipoxygenase-2 after 15 mins by UV-vis spectrophotometer analysis | Homo sapiens | 10 | ALA1268899 | single protein format | Scientific Literature | |
8. | ALA1614397 | Functional | PUBCHEM_BIOASSAY: Confirmation qHTS Assay for Inhibitors of 15-hLO-2 (15-human lipoxygenase 2). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2312, AID2702, AID881] | Homo sapiens | 15 | ALA1201862 | assay format | PubChem BioAssays | |
9. | ALA1817026 | Binding | Inhibition of human reticulocyte N-terminally His6-tagged 15-lipoxygenase-2 assessed as conjugated diene product formation using arachidonic acid by UV-vis spectrophotometer analysis | Homo sapiens | 7 | ALA1811753 | single protein format | Scientific Literature | |
10. | ALA3371453 | Binding | Inhibition of human reticulocyte 15-lipoxygenase-2 by UV-visible spectrophotometry | Homo sapiens | 4 | ALA3352671 | single protein format | Scientific Literature | |
11. | ALA1613800 | Functional | PUBCHEM_BIOASSAY: qHTS Assay for Inhibitors of 15-hLO-2 (15-human lipoxygenase 2). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2312, AID2537, AID2702] | Homo sapiens | 6,916 | ALA1201862 | assay format | PubChem BioAssays | |
12. | ALA1614180 | Functional | PUBCHEM_BIOASSAY: Cuvette-Based Assay fo Inhibitors of 15-hLO-2 (15-human lipoxygenase 2). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2312, AID2537, AID881] | Homo sapiens | ⋯ | ALA1201862 | assay format | PubChem BioAssays | |
13. | ALA2398983 | Binding | Inhibition of N-terminal His6-tagged human prostate epithelial 15-LOX2 using arachidonic acid as substrate assessed as residual activity at 20 uM measured 2 mins post initiation of pseudoperoxidase assay by UV/vis spectrophotometric analysis in presence of 13-(S)-HPODE | Homo sapiens | 4 | ALA2396636 | single protein format | Scientific Literature | |
14. | ALA2399110 | Binding | Inhibition of N-terminal His6-tagged human prostate epithelial 15-LOX2 pseudoperoxidase activity assessed as decomposition of 13-(S)-HPODE at 20 uM measured over 30 mins by UV-vis spectrophotometric analysis | Homo sapiens | 2 | ALA2396636 | single protein format | Scientific Literature | |
15. | ALA2399113 | Binding | Inhibition of N-terminal His6-tagged human prostate epithelial 15-LOX2 pseudoperoxidase activity assessed as reduction of active ferric ion to inactive ferrous state measured decomposition of 13-(S)-HPODE at 40 uM after 30 mins by iron-xylenol orange staining assay | Homo sapiens | 6 | ALA2396636 | single protein format | Scientific Literature | |
16. | ALA3119998 | Binding | Inhibition of N-terminal His6-tagged human epithelial 15-LOX2 using arachidonic acid as substrate by UV-vis spectrophotometric analysis | Homo sapiens | 4 | ALA3112506 | single protein format | Scientific Literature | |
17. | ALA3269368 | Binding | Inhibition of N-terminal His6-tagged human epithelial 15-lipoxygenase-2 using arachidonic acid as substrate by UV-vis spectrometric analysis | Homo sapiens | 4 | ALA3259653 | single protein format | Scientific Literature | |
18. | ALA4417326 | Binding | Inhibition of human recombinant N-terminal His-tagged 15-LOX2 expressed in Escherichia coli using arachidonic acid as substrate preincubated for 5 mins followed by substrate addition measured after 20 mins in dark by ferric ion oxidation-xylenol orange assay | Homo sapiens | 30 | ALA4414666 | assay format | Scientific Literature | |
19. | ALA4417327 | Binding | Inhibition of human recombinant N-terminal His-tagged 15-LOX2 expressed in Escherichia coli assessed as residual activity at 42 uM using arachidonic acid as substrate preincubated for 5 mins followed by substrate addition measured after 20 mins in dark by ferric ion oxidation-xylenol orange assay relative to control | Homo sapiens | 30 | ALA4414666 | assay format | Scientific Literature | |
20. | ALA4825389 | Binding | Inhibition of human 15-LOX-2 assessed as enzymatic rate using arachidonic acid as substrate by UV/Vis spectrophotometric analysis | Homo sapiens | 5 | ALA4823318 | single protein format | Scientific Literature |