| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5251172 | B | Inhibition of UCHL5 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 2. | ALA3412385 | B | Inhibition of GST-tagged UCHL5 (unknown origin) using Ub-AMC as substrate preincubated for 30 mins before substrate addition measured after 60 mins by fluorescence assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 3. | ALA4358872 | B | Inhibition of UCH-37 in human HeLa crude cell extracts assessed as induction of formation of high molecular weight complexes with USP14 at 10 uM incubated for 2 hrs by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA4358873 | B | Inhibition of UCH-37 in intact human HeLa cells assessed as induction of formation of high molecular weight complexes with USP14 at 10 uM incubated for 2 hrs by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4358879 | B | Inhibition of UCH-37 in human HeLa crude cell extracts assessed as induction of formation of high molecular weight complexes with USP14 at 10 uM incubated for 2 hrs in presence of dithiothreitol by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4358880 | B | Inhibition of UCH-37 in intact human HeLa cells assessed as induction of formation of high molecular weight complexes with USP14 at 10 uM incubated for 2 hrs in presence of dithiothreitol by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4358881 | B | Inhibition of UCH-37 in human HeLa crude cell extracts assessed as induction of formation of high molecular weight complexes with USP14 at 10 uM incubated for 2 hrs in presence of glutathione by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4358882 | B | Inhibition of UCH-37 in intact human HeLa cells assessed as induction of formation of high molecular weight complexes with USP14 at 10 uM incubated for 2 hrs in presence of glutathione by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4358897 | B | Covalent binding affinity to UCH-37 in human U2OS cells at 20 uM using probe-1 by ABPP analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4372608 | B | Inhibition of UCHL5 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 11. | ALA4372611 | B | Inhibition of UCHL5 in HEK293T cell lysates assessed as enzyme recovery at 250 uM using HA-tagged vinyl-sulfone as substrate preincubated for 40 mins followed by 10-fold dilution and subsequent substrate addition measured within 15 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 12. | ALA4605992 | B | Inhibition of human GST-tagged UCHL5 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA4687437 | B | Inhibition of UCH-L5 (unknown origin) at 20 uM | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 14. | ALA4688782 | B | Inhibition of N-terminal His6-tagged recombinant human UCHL5 expressed in Escherichia coli BL21 (DE3) codon cells assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate at 200 uM incubated for 30 mins by fluorescence based assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4688783 | B | Inhibition of N-terminal His6-tagged recombinant human UCHL5 expressed in Escherichia coli BL21 (DE3) codon cells assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate incubated for 30 mins by fluorescence based assay | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
| 16. | ALA4688787 | B | Inhibition of UCHL5 in human MDA-MB-231 cells assessed as reduction in enzyme-Ub-VS conjugation at 25 to 50 uM preincubated for 30 mins followed by incubation with Ub-VS for 11 mins in presence of Ub-VS by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4688790 | B | Potency index, ratio of (R)-tiaprofenic acid IC50 to test compound IC50 for inhibition of N-terminal His6-tagged recombinant human UCHL5 expressed in Escherichia coli BL21 (DE3) codon cells incubated for 30 mins by fluorescence based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA4887154 | B | UCHL5 inhibition assay with Ub-Rho-morpholine as substrate | Homo sapiens | 1 | single protein format | Donated Chemical Probes - SGC Frankfurt | ||
| 19. | ALA5108422 | B | Inhibition of UCHL5 in human HEK293 cells using HA-Ub-VME as substrate at 0.1 to 10 uM incubated for 1 hr by immunoblotting analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 20. | ALA5153787 | B | Inhibition of UCH-L5 (unknown origin) at 10 uM using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by microplate spectrophotometer analysis | Homo sapiens | 6 | single protein format | Scientific Literature |
The page will load shortly, Thanks for your patience!