| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1110577 | B | Inhibition of human recombinant aurora B/INCENP complex after 120 mins by IMAP fluorescence polarization assay | Homo sapiens | 9 | protein complex format | Scientific Literature | ||
| 2. | ALA2014528 | B | Inhibition of AurB-INCENP at 10 uM | 1 | protein complex format | Scientific Literature | |||
| 3. | ALA3362284 | B | Inhibition of human Aurora B/ INCENP using 2 uM ATP after 30 mins by IMAP/summary (Abse5) assay | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 4. | ALA3411673 | B | Inhibition of AURKB/INCENP (unknown origin) by FRET analysis | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 5. | ALA3411679 | B | Inhibition of AURKB/INCENP (unknown origin) by cell-based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3423899 | B | Inhibition of AURKB/INCENP (unknown origin) preincubated for 15 mins prior to ATP addition by FRET-based homogenous assay | Homo sapiens | 45 | protein complex format | Scientific Literature | ||
| 7. | ALA3423976 | B | Inhibition of AURKB/INCENP (unknown origin) transfected in human HCT116 cells after 4 hrs by near infrared imager analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3705953 | B | Radioactive Assay: The capacity to inhibit the kinase activity of the enzyme is estimated by measuring the residual kinase activity of the enzyme in the presence of different concentrations of the test compound (generally from 0.17 to 10 000 nM). A dose-response curve is produced, which allows an IC50 (50% inhibitory concentration) to be determined. The kinase activity is measured by a radioactive assay of the amount of radioactive phosphate (33P) incorporated into a fragment of the protein NuMA (Nuclear Mitotic Apparatus protein) after 30 minutes of incubation at 37° C. The test compound is first dissolved at different concentrations in dimethyl sulphoxide (DMS (J). Reaction takes place in the wells of a FlashPlate microtiter plate (Nickel Chelate FlashPlate-96, PerkinElmer). Each well (100 μl) contains 10 nM Aurora A, 500 nM NuMA, 1 μM ATP and 0.2 μCi ATP-γ-33P in a buffer of 50 mM Tris-HCl, pH=7.5; 10 mM MgCl2; 50 mM NaCl, 1 mM dithiothreitol. The final percentage of DMSO is 3%. | Homo sapiens | 10 | protein complex format | BindingDB Database | ||
| 9. | ALA3811383 | B | Inhibition of full-length human N-terminal GST-fused Aurora B (1 to 344 residues)/His-tagged INCENP (803 to 918 residues) using 5FAM-LRRASLG-CONH2 as substrate after 60 mins | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 10. | ALA3820512 | B | Inhibition of recombinant human His-tagged Aurora B/full length recombinant human N-terminal GST-tagged INCENP (821-end residues) expressed in baculovirus infected Sf21 cells by radiometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA3862725 | B | Inhibition of Aurora-B/INCENP (unknown origin) using biotinylated STK2 substrate incubated for 30 mins by HTRF assay | Homo sapiens | 13 | protein complex format | Scientific Literature | ||
| 12. | ALA3882307 | B | Inhibition of human full-length N-terminal GST-tagged Aurora B/His-tagged INCENP (803 to 916 residues) using kemptide as substrate and [gamma33P]ATP after 45 mins by liquid scintillation counting method | Homo sapiens | 37 | protein complex format | Patent Bioactivity Data | ||
| 13. | ALA4001360 | B | Inhibition of human AURKB/INCENP | Homo sapiens | 3 | protein complex format | Scientific Literature | ||
| 14. | ALA4024204 | B | Inhibition of recombinant human full length C-terminal His6-tagged Aurora B//N-terminal GST-tagged INCENP (821-end residues) expressed in baculovirus infected sf21 cells using AKRRRLSSLRA substrate in presence of [gamma33P]ATP after 10 mins by scintillation counting method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4256643 | B | Inhibition of human full length N-terminal GST-tagged Aurora B (1 to 344 end residues)/His-tagged INCENP (803 to 918 end residues) expressed in baculovirus expression system using fluorescence-labeled FL-Peptide 21 as substrate after 60 mins in presence of ATP by fluorescence assay based Cheng-Prusoff equation analysis | Homo sapiens | 24 | assay format | Scientific Literature | ||
| 16. | ALA4266269 | B | Inhibition of Aurora B/INCENP (unknown origin) assessed as affinity constant pre-incubated for 1 hr followed by FL-PKAtide substrate and ATP addition | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 17. | ALA4266270 | B | Inhibition of Aurora B/INCENP (unknown origin) assessed as inhibitor-enzyme complex half life pre-incubated for 1 hr followed by FL-PKAtide substrate and ATP addition | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 18. | ALA4266271 | B | Inhibition of Aurora B/INCENP (unknown origin) pre-incubated for 1 hr followed by FL-PKAtide substrate and ATP addition | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 19. | ALA4266323 | B | Inhibition of human Aurora B/INCENP pre-incubated for 30 mins before 5-FAM-PKAtide substrate addition and measured after 120 mins | Homo sapiens | 1 | protein complex format | Scientific Literature | ||
| 20. | ALA4266325 | B | Inhibition of human Aurora B/INCENP assessed as dissociation half life pre-incubated for 30 mins before 5-FAM-PKAtide substrate addition and measured after 120 mins | Homo sapiens | 1 | protein complex format | Scientific Literature |
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